FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences789383
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34980.44313090096949137No Hit
GAGTTACAGTACCCCTTGGACGTTCGGCCAGGGGACCAAGGTAGAAATCAAACGAACTGTGGCTGCACCAT28670.36319505233834526No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25250.3198700757427003No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24800.3141694209274839No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21440.27160453164053444No Hit
GTACTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT21390.27097112554995484No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19340.24500147583619106No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19140.24246785147387265No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18120.2295463672260487No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17800.22549256824633923No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCACCTATTTAAATTGGTATCGGCAGAGA17770.22511252459199146No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17480.22143876926662975No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16750.21219104034416753No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGTTGCAGAGTACTGATGGTGAGAGTGAAATCTGTC16740.2120643591260516No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16110.20408344238474857No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAC15920.20167649924054612No Hit
GATCTATGCTACATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA14970.18964178351953362No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14850.18812160890214258No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14850.18812160890214258No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14750.18685479672098335No Hit
GTAATACACGGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATACA14670.18584134697605598No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14350.1817875479963465No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14110.17874719876156442No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14040.17786043023475295No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13940.17659361805359375No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13670.17317322516446387No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACTTCCAGATGACCCAGTC13630.1726665002920002No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13500.17101964445649323No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCTACCTTGGTCCCCTGGCCGAACGTCCAAGGGGTA13280.16823265765794299No Hit
GTGCAGCCACAGTTCGTTTGATTTCTACCTTGGTCCCCTGGCCGAACGTC13190.16709252669489968No Hit
GACCAGGGAAAGCCCCTGACCTCCTGATCTATGCTACATCCAATTTGCAA12810.16227864040649467No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12670.1605051033528718No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12600.15961833482606033No Hit
ATGCTACATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA12250.15518449219200312No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11890.15062396833982997No Hit
GATTTCACTCTCACCATCAGTACTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACC11890.15062396833982997No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCCTTGGACGTTCGGCCAGGGGACCAAGGTAGAAATCAAACGAA11860.1502439246854822No Hit
CCATCAGTACTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCAACAG11840.14999056224925036No Hit
ACCAAATACTACGCAGGCTCTGTGAAGGGCCGATTTACCATCTCCAGGGACAACGCCAAGAACTCACTGTATCTG11710.1483437064137434No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACTT11150.1412495581992518No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA10930.13846257140070156No Hit
CATCAGTACTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACCCCTTGGACGTTCGG10810.13694239678331052No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10360.13124174196809407No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10290.13035497344128263No Hit
ACAGTACCCCTTGGACGTTCGGCCAGGGGACCAAGGTAGAAATCAAACGA10260.12997492978693487No Hit
GTAGTAAGTTGCAAAATCCTCAGGTTGCAGAGTACTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA10220.1294682049144712No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10190.1290881612601234No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9920.12566776837099355No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9910.12554108715287762No Hit
GTACTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCAACAGAGTTAC9890.1252877247166458No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT9820.12440095618983434No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9790.12402091253548657No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACTTCCAGATGACCCAGTCTCCATCCTCC9590.12148728817316817No Hit
GTGTATTACTGTGTGAGAATCTGGTGGCGTATGCTTCAGGGAGTTCCCCT9580.12136060695505224No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9500.12034715721012487No Hit
CAGTAATACACGGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATA9490.12022047599200894No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9380.11882698259273383No Hit
CTCCTGATCTATGCTACATCCAATTTGCAAAGTGGGGTCCCATCAAGGTT9320.1180668952840383No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9270.1174334891934587No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9190.11642003944853131No Hit
GGGAAAGCCCCTGACCTCCTGATCTATGCTACATCCAATTTGCAAAGTGG9150.11591331457606764No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA9000.11401309630432882No Hit
AGCCTGGAGGGTCCCTGAGACTCTCCTGTGGAGCCTCTGGATTCACCTTC8890.1126196029050537No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8880.11249292168693777No Hit
GGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGTTGCAGAGTACTGATGGTGAGAGTGAAAT8790.11135279072389448No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC8480.10742567296230093No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8320.1053987734724462No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8280.10489204859998251No Hit
AGTCAGAGCATTAGCACCTATTTAAATTGGTATCGGCAGAGACCAGGGAA8250.10451200494563476No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8240.10438532372751884No Hit
GTATTTGGTAGTACCACTATCACTAATGTGAGAAATCCACTGCAGCCCCTTCCCTGGGGCCTGGCGAATCCAACC8150.10324519276447555No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8100.10261178667389595No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8070.10223174301954817No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8070.10223174301954817No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG8060.10210506180143225No Hit
CTCCTGTGGAGCCTCTGGATTCACCTTCAGTGATTATTATATGGGTTGGATTCGCCAGGCCCCAGGGAAGGGG7990.10121829327462081No Hit
GATCAGGAGGTCAGGGGCTTTCCCTGGTCTCTGCCGATACCAATTTAAAT7970.10096493083838896No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7950.10071156840215711No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA150.002487179868.058191
CGCTATC150.002492810868.019397
TTCGCAC306.4276316E-445.828237
GGTATCA8050.040.581281
GTATCAA20100.036.568581
GTGGTAT2850.035.82011
TTAACGG400.002410919635.0236850
TAACGGC450.004270087531.16672951
GAGTTTA2650.030.8188041
TATCAAC23950.030.3943962
CTCCTAT450.004943170630.2480851
ATAGAAC450.00495544230.2327563
ATCAACG24000.030.0437973
TCAACGC24350.029.6119584
AACGCAG25000.028.9780946
TAGTCAC953.274945E-728.9775238
CAACGCA24900.028.9578765
CGGTACC500.00658574128.5232460
TGTTAGA9500.027.50537370
AGACTCG500.00829027627.2094786
Produced by FastQC (version 0.11.5)