FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences877195
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATGCTGGAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATCGTGAGAGTGAATTCTGTC30680.3497511955722502No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29360.3347032301825706No Hit
GCATAATAGTTACCCTTTTTCTTTCGGCCCTGGGACCAAAGTGGATATCAGACGAACTGTGGCTGCACCAT27770.3165772718722747No Hit
GATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA27620.31486727580526563No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24880.28363134764790043No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21800.24851942840531466No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20800.23711945462525438No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20320.23164746721082544No Hit
GTAATACACAGCCGTGTCTTCGGCTCTCAGGTTATTCATTTGCAGATACA20140.22959547193041455No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19710.22469348320498864No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18530.2112415141445175No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17100.19493955163903123No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16890.19254555714521857No Hit
GCATAGATCAGGCGCTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGCC16040.1828555794321673No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15440.17601559516413112No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG15140.17259560303011304No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14920.17008760879849977No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14370.16381762321946658No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14320.1632476245304636No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14170.16153762846345454No Hit
GTGTATTACTGTGCGAGATCCGTTTGGGATATTGTAATAATATCAGCTGC13950.15902963423184127No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACACAGTCACCATCACTT13500.15389964603081413No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13290.15150565153700146No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13090.14922565678098942No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12790.1458056646469713No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12400.14135967487274778No Hit
GAATTCACTCTCACGATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCTCCAGCATAATAGTTAC12210.13919367985453635No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12190.1389656803789351No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12100.1379396827387297No Hit
ACTTATTACTGTCTCCAGCATAATAGTTACCCTTTTTCTTTCGGCCCTGGGACCAAAGTGGATATCAGACGAA11970.13645768614732187No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11720.13360769270230677No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA11570.13189769663529774No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11220.12790770581227665No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11200.1276797063366754No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10820.12334771630025251No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10690.12186571970884466No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9900.11285974042259703No Hit
GTGCAGCCACAGTTCGTCTGATATCCACTTTGGTCCCAGGGCCGAAAGAA9860.11240374147139462No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT9760.11126374409338859No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9760.11126374409338859No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATCGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG9710.11069374540438558No Hit
GTAGGAGACACAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGAAATGATTTAGGCTGGTTTCAGCAGAAA9700.11057974566658496No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT9640.10989574723978136No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG9420.10738775300816808No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC9350.10658975484356387No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9230.10522175798995662No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8990.10248576428274214No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8930.10180176585593853No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8920.10168776611813791No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCAGGTGTGACAT8830.10066176847793251No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9100.042.6319271
GTATCAA22100.035.8785741
TATCAAC24300.032.197322
ATCAACG24850.031.477523
TCAACGC25050.031.3619694
CAACGCA25400.030.9280515
CTATACA553.5416454E-430.9176434
ACGAATC450.004671189530.60046237
AACGCAG25850.030.521216
AGAGTAC27600.028.46277411
CGCAGAG27900.028.4005058
ACGCAGA27900.028.2786147
CAGAGTA28150.027.90666210
CGAATCT500.007771945627.5722438
GTTGACG500.00826832727.22461
TTATATC500.00829367227.2075254
TTCGGCT4900.027.08415619
GCAGAGT29350.026.76729
TGTTAGA8850.026.53547770
CGATTCA4950.026.35678970
Produced by FastQC (version 0.11.5)