FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1601870
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGCTCACCTCCCACGTTCGGCCAAGGGA68380.42687608857148207No Hit
GTAATAAACAGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCATATACA50420.31475712760710917No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48720.30414453107930106No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48130.30046133581376766No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCAGTGCCA45120.2816707972557074No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGCTACCA37630.23491294549495279No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG37340.23310256138138552No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35720.22298938116076836No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACCTCCCACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAA33710.21044154644259522No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32030.19995380399158483No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31670.19770643060922546No Hit
GTTTATTACTGTGCGAGAGGGGACTGTGGTGGTGGTGACTGCGAAGACCCCTACTGGTACTTCGATCTCTGGGGC29440.18378520104627716No Hit
GTATGGTAGCTCACCTCCCACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCAT29300.18291122250869296No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28390.17723036201439568No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28280.1765436645920081No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG28110.17548240493922726No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA27610.1723610530192837No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27480.1715495015200984No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27160.1695518362913345No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27140.16942698221453675No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT27000.16855300367695256No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26830.16749174402417175No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT26480.16530679768021125No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCAGTGCCA26220.1636836946818406No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25740.16068719683869478No Hit
GTCCCAGACCCAGTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT25040.15631730415077377No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24890.1553808985747907No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC23770.14838907027411713No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACTCTCTCCTGCAGGGCCAGTCACA23610.1473902376597352No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23530.14689082135254422No Hit
GTCACAGTGTTGGCAGCAACTTCTTAGCCTGGTACCAGCGGAAACCTGGCCAGGCTCCCAGACTCCTCATCCATA23310.14551742650776905No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT23290.1453925724309713No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC22780.14220879347262885No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACTCTCTCCTGCAGGGCCAGTCACAGTGTTGGCAG22260.13896258747588755No Hit
CATCCATACTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCACTGGGTCTGGGACAGACTTCA21990.13727705743911803No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21620.1349672570183598No Hit
GTGTTGGCAGCAACTTCTTAGCCTGGTACCAGCGGAAACCTGGCCAGGCTCCCAGACTCCTCATCCATACTGCAT21460.13396842440397785No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAATGTGAGGTGCAGTTGGTGGAGT21320.13309444586639366No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT20840.1300979480232478No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCC20750.129536104677658No Hit
CTCCCAGACTCCTCATCCATACTGCATCCAGCAGGGCCACTGGCATCCCA20540.12822513687128168No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACTCTCTCCTGC20270.12653960683451218No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20250.12641475275771444No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19920.12435466049055166No Hit
CTCCTGCAGGGCCAGTCACAGTGTTGGCAGCAACTTCTTAGCCTGGTACC19860.12398009826015843No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18790.1173004051514792No Hit
TCCATACTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCACTGGGTCTGGGACAGACTTCACT18290.11417905323153564No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC18110.11305536654035596No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC18090.11293051246355822No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC17990.11230624207956952No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCA17830.11130740946518757No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGCTACCACCGGAGAGATTGTGTTGACGCAGTCTC17590.10980916054361466No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC17420.10874790089083385No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTG17410.10868547385243497No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17290.1079363493916485No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAG17140.10699994381566545No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT17090.10668780862367108No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGCTAC16820.10500227858690156No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16600.10362888374212638No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT16590.10356645670372752No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16590.10356645670372752No Hit
GAGTGAAGTCTGTCCCAGACCCAGTGCCACTGAACCTGTCTGGGATGCCA16550.10331674855013204No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16320.101880926666958No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG16280.10163121851336251No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT16250.1014439373981659No Hit
CTCCTGTGCAGGCTCTGGATTCACCTTTAGTCGTTATGAAATGAACTGGG16060.10025782366858735No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG16030.10007054255339073No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC200.007686991751.183331
TCGTATG5350.048.3604840
ATAGCGA400.0096655348.29925570
CGTATGC5600.048.12708741
CGCTCAT5900.047.00724832
TAGCGCT6200.046.8550929
CTAGCGC6200.046.83897428
ATCTCGT5300.046.66953737
GCCGTCT5400.046.31350346
TTATACA6500.046.1904682
ATGCCGT5750.045.82773644
ACTAGCG6350.045.7182527
GCGCTCA6300.045.62167731
GACTAGC6400.045.34123226
CTTATAC6650.044.6410981
ACGAGAC6650.044.6369222
CGAGACT6700.044.3079623
CACGAGA6750.043.97425521
TACACAT6850.043.830375
GGTATCA15200.043.775221
Produced by FastQC (version 0.11.5)