FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004688838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1601870
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64780.40440235474788844No Hit
GTATTACTGTCAGCAGTATGGTAGCTCACCTCCCACGTTCGGCCAAGGGA62960.3930406337592938No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49370.3082022885752277No Hit
GTAATAAACAGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCATATACA45440.28366846248447125No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCAGTGCCA42070.26263055054405166No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39910.24914631024989545No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG39900.24908388321149655No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38700.24159263860363203No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGCTACCA35620.22236511077677965No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACCTCCCACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAA31750.1982058469164164No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA30740.19190071603813044No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30570.1908394563853496No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29030.18122569247192344No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC28270.17648123755360923No Hit
GTATGGTAGCTCACCTCCCACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCAT28180.1759193942080194No Hit
GTTTATTACTGTGCGAGAGGGGACTGTGGTGGTGGTGACTGCGAAGACCCCTACTGGTACTTCGATCTCTGGGGC27980.17467085344004196No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27850.1738593019408566No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27390.17098765817450853No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27190.1697391174065311No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT26780.1671796088321774No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26370.16462010025782367No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25610.15987564533950946No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCAGTGCCA25350.1582525423411388No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG24560.15332080630762795No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23290.1453925724309713No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACTCTCTCCTGCAGGGCCAGTCACA23160.144581020931786No Hit
GTCCCAGACCCAGTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT23090.1441440316629939No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22940.14320762608701082No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22510.14052326343585933No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAATGTGAGGTGCAGTTGGTGGAGT22300.13921229562948303No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22160.13833831709189884No Hit
GTGTTGGCAGCAACTTCTTAGCCTGGTACCAGCGGAAACCTGGCCAGGCTCCCAGACTCCTCATCCATACTGCAT21880.13659036001673044No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACTCTCTCCTGCAGGGCCAGTCACAGTGTTGGCAG21720.1355915274023485No Hit
GTCACAGTGTTGGCAGCAACTTCTTAGCCTGGTACCAGCGGAAACCTGGCCAGGCTCCCAGACTCCTCATCCATA21710.13552910036394963No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21370.133406581058388No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21170.1321580402904106No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20580.12847484502487716No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT20540.12822513687128168No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCC20240.12635232571931557No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACTCTCTCCTGC19760.12335582787616974No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC19730.12316854676097311No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC19340.12073389226341714No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19180.11973505964903519No Hit
CTCCCAGACTCCTCATCCATACTGCATCCAGCAGGGCCACTGGCATCCCA19020.11873622703465325No Hit
CATCCATACTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCACTGGGTCTGGGACAGACTTCA18960.11836166480426003No Hit
CTCCTGCAGGGCCAGTCACAGTGTTGGCAGCAACTTCTTAGCCTGGTACC17900.11174439873397966No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC17760.11087042019639545No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTG17730.11068313908119884No Hit
GAGTGAAGTCTGTCCCAGACCCAGTGCCACTGAACCTGTCTGGGATGCCA17560.10962187942841804No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC17400.1086230468140361No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17270.10781149531485076No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCA17160.1071247978924632No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT17100.10675023566206994No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAG17090.10668780862367108No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT16990.10606353823968237No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC16700.1042531541261151No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG16550.10331674855013204No Hit
TCCATACTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCACTGGGTCTGGGACAGACTTCACT16540.10325432151173317No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16500.10300461335813768No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16330.10194335370535687No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT16320.101880926666958No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGCTACCACCGGAGAGATTGTGTTGACGCAGTCTC16180.10100694812937379No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC16150.10081966701417718No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC16060.10025782366858735No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)