FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1106757
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACTCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG53340.4819486120259461No Hit
GAGTATTACTGTGCAGCGTGGGATGCCAGCCTGGATGGTGTATTATTCGG50790.4589083240494526No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC41870.3783124931669734No Hit
GTAATAGACGGCCGTGTCCTCGGCTCTTAGGGTGTTCATTTGCAAATAAA38880.3512966260886536No Hit
GTATTACTGTGCAGCGTGGGATGCCAGCCTGGATGGTGTATTATTCGGCG34660.31316720833931927No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTG33550.3031379065142574No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33010.2982587867074705No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31870.28795842267092053No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATAATACACCATCCAGGCTGGCATCCCACG31830.2875970063889363No Hit
GTGTATTATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC27810.25127467004952303No Hit
GTCTATTACTGTGCGAAAGATCGCTACCATGACTACGGTGGTAACTCCGG26840.24251032521140592No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC25950.23446881293725721No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25920.23419775072576907No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATAATACACCATCCAGG25760.2327520855978322No Hit
CAGTAATACTCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGA24410.22055428608086508No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT24080.21757260175449536No Hit
CCCCCAGACTCCTCATCTATTCAACTAATTACCGGCCCTCAGGGGTCCCT23230.20989250576233087No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23120.20889861098687426No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22930.20718188364744924No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22920.2070915295769532No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT22350.2019413475586782No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22010.1988693091618124No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21430.19362877307304133No Hit
ATAATACACCATCCAGGCTGGCATCCCACGCTGCACAGTAATACTCAGCC20620.18631009336286106No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG20020.18088884913309788No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19500.17619043746730312No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG19450.17573866711482283No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19150.1730280449999413No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19040.17203415022448468No Hit
CCCTAAGAGCCGAGGACACGGCCGTCTATTACTGTGCGAAAGATCGCTAC18980.17149202580150838No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18880.17058848509654784No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC18860.17040777695555573No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG18850.1703174228850597No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18780.16968494439158732No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18610.1681489251931544No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18460.1667936141357136No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18320.16552865714876888No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18240.16480582458480045No Hit
GTCTGGTACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG17730.16019776698950175No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16960.1532405035613057No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16930.15296944134981752No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC16770.15152377622188068No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC16510.14917457038898332No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16140.14583146978062936No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT16100.14547005349864514No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15720.14203659881979513No Hit
GTCTGGGGGCGGTTCCTGGGAATTGCTGGTACCAGTAGACAAAGTTAGTT15680.14167518253781092No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15280.1380610197179688No Hit
ATGCAAACTCCGTGAAGGGCCGGTTCACCATCTCCAGGGACGATTCCAAGAACACACTTTATTTGCAAATGAACA15260.1378803115769767No Hit
GTACCAGACTTGGAGCCAGAGAAGCGGTCAGGGACCCCTGAGGGCCGGTA15240.13769960343598459No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG15120.13661535459003196No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA14960.13516968946209512No Hit
GGACTGGAGTGGGTCTCAGGTATCACAGCTAGTCCTCACAGCACACACTA14270.12893525859786745No Hit
GCACAGTAATACTCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTACCAGACTTGGAG14260.1288449045273714No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGCTCTGGAAGCAGCTCCAACATCGGAACTAA14090.1273088853289385No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT14080.12721853125844246No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14020.12667640683546613No Hit
GATGAGGAGTCTGGGGGCGGTTCCTGGGAATTGCTGGTACCAGTAGACAA14010.1265860527649701No Hit
CTCTTGCTCTGGAAGCAGCTCCAACATCGGAACTAACTTTGTCTACTGGTACCAGCAATTCCCAGGAACCGCCCC13950.12604392834199377No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13660.12342366029760823No Hit
CAGCACACACTATGCAAACTCCGTGAAGGGCCGGTTCACCATCTCCAGGG13580.12270082773363981No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC13570.12261047366314377No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC13390.12098410039421481No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13360.12071303818272665No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13340.12053233004173454No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC13140.11872524863181348No Hit
GTATTATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG13050.11791206199734902No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12860.116195334657924No Hit
CCCCTGAGGGCCGGTAATTAGTTGAATAGATGAGGAGTCTGGGGGCGGTTCCTGGGAATTGCTGGTACCAGTAGA12840.1160146265169319No Hit
TTACTGTGCAGCGTGGGATGCCAGCCTGGATGGTGTATTATTCGGCGGAG12750.11520143988246742No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT12730.11502073174147533No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12720.11493037767097926No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12610.11393648289552269No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12600.11384612882502662No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12530.11321365033155426No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG12470.11267152590857794No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12450.11249081776758584No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12380.11185833927411348No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG12280.11095479856915295No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12270.11086444449865689No Hit
CTCCTCATCTATTCAACTAATTACCGGCCCTCAGGGGTCCCTGACCGCTT12180.11005125786419243No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA12170.10996090379369636No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGGAAGAGACGGTGACCATTGACCCTTGGCCCCAGACATCAAAGGCA12170.10996090379369636No Hit
CTATGCAAACTCCGTGAAGGGCCGGTTCACCATCTCCAGGGACGATTCCAAGAACACACTTTATTTGCAAATGAA12160.10987054972320032No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12080.10914771715923188No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATAATACAC12070.10905736308873583No Hit
CTCCTGCGCAGCCTCTGGATTCACCTTTAACGCCTACGCCATGGGCTGGGCCCGCCAGGCTCCAGGGAAGGGA12020.10860559273625557No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12010.1085152386657595No Hit
CCTTGGTCCCTCCGCCGAATAATACACCATCCAGGCTGGCATCCCACGCTGCACAGTAATACTCAGCCTCATCCT11930.10779240610179108No Hit
CCATTGACCCTTGGCCCCAGACATCAAAGGCACTTCCGGAGTTACCACCG11900.10752134389030293No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11890.10743098981980687No Hit
CAGTAATAGACGGCCGTGTCCTCGGCTCTTAGGGTGTTCATTTGCAAATA11810.10670815725583845No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11790.10652744911484634No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11780.1064370950443503No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11680.10553355433938977No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11610.10490107584591739No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG11310.1021904537310358No Hit
GAATAATACACCATCCAGGCTGGCATCCCACGCTGCACAGTAATACTCAGCCTCATCCTCGGACCGGAGCCCA11310.1021904537310358No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11130.10056408046210685No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC11090.10020266418012264No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTG150.002176634570.38792458
GTTATAC200.007524417251.4597783
TAAGCGG200.00752979651.4504813
GTGGTAT1150.044.7597731
TCGTATG1300.042.5696840
ACCGTAC1350.040.6908427
TCTCGTA1300.039.83104338
CGTATGC1400.039.55421441
ATCTCGT1350.038.33838337
CTAGATC1350.038.24625433
GTATAAT451.0336101E-438.1286931
ATGCCGT1500.037.00499344
GACCGTA1500.036.61347626
CACGAGA1500.036.59031321
GATCTCG1450.035.6651836
ACGAGAC1550.035.41478322
GACAGGT2250.035.07841
GTATCAA17200.034.7148481
TAACGGC709.788109E-734.47633736
TTAACGG709.815958E-734.46381835
Produced by FastQC (version 0.11.5)