FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004688840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1106757
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTATTACTGTGCAGCGTGGGATGCCAGCCTGGATGGTGTATTATTCGG50200.45357743389018546No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTG37590.3396409509946628No Hit
GTAATAGACGGCCGTGTCCTCGGCTCTTAGGGTGTTCATTTGCAAATAAA36770.3322319172139865No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC36350.32843704625315223No Hit
GTATTACTGTGCAGCGTGGGATGCCAGCCTGGATGGTGTATTATTCGGCG34450.31126977285890217No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32440.29310860468919553No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30030.27133327369964677No Hit
GTGTATTATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC29540.2669059242453402No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATAATACACCATCCAGGCTGGCATCCCACG28990.26193645036805735No Hit
GTCTATTACTGTGCGAAAGATCGCTACCATGACTACGGTGGTAACTCCGG26880.24287174149339014No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT26620.24052253566049278No Hit
GTAATACTCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG25870.2337459803732888No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24880.2248009273941796No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24840.22443951111219537No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23760.21468127149862165No Hit
CCCCCAGACTCCTCATCTATTCAACTAATTACCGGCCCTCAGGGGTCCCT23460.21197064938374005No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC22860.20654940515397688No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22720.20528444816703215No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG22250.20103780685371767No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATAATACACCATCCAGG22080.19950178765528476No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21890.19778506031585974No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT21620.19534550041246634No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA20550.18567761486938866No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20400.1843223038119479No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC20090.18152132762657022No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19880.17962389214615312No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19720.17817822701821628No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19710.17808787294772022No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC19630.1773650403837518No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA18860.17040777695555573No Hit
GTCTGGTACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG18140.1639022838798399No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17810.16092059955347018No Hit
ATAATACACCATCCAGGCTGGCATCCCACGCTGCACAGTAATACTCAGCC17810.16092059955347018No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG17570.1587521018615649No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG17320.1564932500991636No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG17170.1551379390417228No Hit
GTCTGGGGGCGGTTCCTGGGAATTGCTGGTACCAGTAGACAAAGTTAGTT16630.15025881923493592No Hit
CCCTAAGAGCCGAGGACACGGCCGTCTATTACTGTGCGAAAGATCGCTAC16620.15016846516443988No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16230.14664465641509383No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16200.14637359420360566No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16120.14565076163963725No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15840.14312084766574776No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15450.1395970389164017No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15230.13760924936548855No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15210.13742854122449644No Hit
CTCTTGCTCTGGAAGCAGCTCCAACATCGGAACTAACTTTGTCTACTGGTACCAGCAATTCCCAGGAACCGCCCC15130.136705708660528No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGCTCTGGAAGCAGCTCCAACATCGGAACTAA15130.136705708660528No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14970.13526004353259116No Hit
ATGCAAACTCCGTGAAGGGCCGGTTCACCATCTCCAGGGACGATTCCAAGAACACACTTTATTTGCAAATGAACA14700.13282048362919774No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC14670.13254942141770956No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14490.1309230481487806No Hit
CTCCTCATCTATTCAACTAATTACCGGCCCTCAGGGGTCCCTGACCGCTT14430.13038092372580432No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT14410.1302002155848122No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14240.1286641963863793No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT14070.1271281771879464No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13940.12595357427149773No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13860.1252307417075293No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13830.12495967949604114No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13710.1238754306500885No Hit
GTACCAGACTTGGAGCCAGAGAAGCGGTCAGGGACCCCTGAGGGCCGGTA13680.12360436843860034No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13650.1233333062271122No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13500.1219779951696714No Hit
GATGAGGAGTCTGGGGGCGGTTCCTGGGAATTGCTGGTACCAGTAGACAA13480.12179728702867929No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13390.12098410039421481No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13350.12062268411223061No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13300.12017091375975034No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC13290.12008055968925428No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12990.1173699375743727No Hit
CTCCTGCGCAGCCTCTGGATTCACCTTTAACGCCTACGCCATGGGCTGGGCCCGCCAGGCTCCAGGGAAGGGA12810.11574356430544375No Hit
GTATTATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG12620.11402683696601873No Hit
CTATGCAAACTCCGTGAAGGGCCGGTTCACCATCTCCAGGGACGATTCCAAGAACACACTTTATTTGCAAATGAA12580.11366542068403451No Hit
CAGCACACACTATGCAAACTCCGTGAAGGGCCGGTTCACCATCTCCAGGG12520.1131232962610582No Hit
GGACTGGAGTGGGTCTCAGGTATCACAGCTAGTCCTCACAGCACACACTA12430.11231010962659373No Hit
TTACTGTGCAGCGTGGGATGCCAGCCTGGATGGTGTATTATTCGGCGGAG12370.11176798520361741No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12330.1114065689216332No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC12150.10978019565270425No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC12090.10923807122972794No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11990.10833453052476741No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11900.10752134389030293No Hit
CCCCTGAGGGCCGGTAATTAGTTGAATAGATGAGGAGTCTGGGGGCGGTTCCTGGGAATTGCTGGTACCAGTAGA11840.10697921946732662No Hit
CAGTAATACTCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGA11720.10589497062137398No Hit
GGGTCACCATCTCTTGCTCTGGAAGCAGCTCCAACATCGGAACTAACTTT11640.10517213805740556No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATAATACAC11510.10399753514095686No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11380.10282293222450818No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11330.10237116187202791No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG11330.10237116187202791No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC11270.10182903744905158No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11260.10173868337855554No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCA11260.10173868337855554No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG11220.10137726709657134No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)