FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933591
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAC46610.4992550270943057No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT44530.4769754635595245No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAAATTATGATGTACACTGGTACCAGCAACTTCCAGGAACAGC43070.4613369237706876No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG40310.43177365677261237No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC38790.41549243726642604No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC36310.3889283422826484No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC33660.36054332143304724No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC33580.35968641514324795No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC33580.35968641514324795No Hit
GACCTAGGACGGTGACCTTGGTCCCACCTCCGAAGACAGAACCACGCAGG30580.32755242927577494No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCACCTCCGAAGACAGAACCACGCAGGGTGCTGTCATACGT29190.31266368249051246No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC28500.30527286574099366No Hit
ACCTAGGACGGTGACCTTGGTCCCACCTCCGAAGACAGAACCACGCAGGG27680.296489576270551No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26340.2821363959164131No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT25010.26789032884850006No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24240.25964260580918197No Hit
GTCATACGTCTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG23850.25546518764641046No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG23790.254822507929061No Hit
GATTATTACTGCCAGACGTATGACAGCACCCTGCGTGGTTCTGTCTTCGG22850.24475385902391947No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC22690.24304004644432092No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT21800.2335069639703039No Hit
ATGGTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGACACCTCAGCCTCCCTGG21790.233399850684079No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG21450.22975799895243207No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG21080.2257948073621104No Hit
ACGTATGACAGCACCCTGCGTGGTTCTGTCTTCGGAGGTGGGACCAAGGT20390.2184039906125916No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG20350.21797553746769194No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG20200.21636883817431832No Hit
TGACAGCACCCTGCGTGGTTCTGTCTTCGGAGGTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCC19960.2137981193049205No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGACGTATGACAGCACCCTGCGTGGTT19820.21229853329777174No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAAATTATGATGTACACTGGTACCAG19460.20844245499367495No Hit
GTGTTACCATAGATGAGGAGTTTGGGGGCTGTTCCTGGAAGTTGCTGGTA19160.20522905640692765No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC18780.2011587515303811No Hit
GTTCTGTCTTCGGAGGTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCC18560.1988022592334331No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA18540.19858803266098324No Hit
GTGATGGCCAGGGAGGCTGAGGTGTCAGACTTGGAGCCAGAGAATCGGTC17970.19248257534616336No Hit
GGATGAGGCTGATTATTACTGCCAGACGTATGACAGCACCCTGCGTGGTTCTGTCTTCGGAGGTGGGACCAAGGT17370.18605577817266877No Hit
GTGCTGTCATACGTCTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAG17300.18530598516909438No Hit
GATGTACACTGGTACCAGCAACTTCCAGGAACAGCCCCCAAACTCCTCAT16510.17684403555732647No Hit
GTCTGACACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATG16460.17630846912620193No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC16210.1736306369705792No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG16110.1725595041083301No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG15890.1702030118113821No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC15890.1702030118113821No Hit
TGGTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGACACCTCAGCCTCCCTGGC15730.16848919923178352No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT15700.16816785937310877No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT15590.16698961322463476No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA15580.16688249993840987No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15520.1662398202210604No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGACGTAT15240.16324064820676293No Hit
GCTCCAACATCGGGGCAAATTATGATGTACACTGGTACCAGCAACTTCCA15140.16216951534451382No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG15120.161955288772064No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGACACCTCAG14960.16024147619246543No Hit
GCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGTC14710.1575636440368427No Hit
GCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGACGTATGACAGCA14490.15520715173989466No Hit
GCCCCCAAACTCCTCATCTATGGTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT14470.15499292516744484No Hit
AGACAGAACCACGCAGGGTGCTGTCATACGTCTGGCAGTAATAATCAGCC14270.15285065944294662No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC14180.15188663986692247No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC14040.15038705385977372No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG14010.150065714001099No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC13900.14888746785262497No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA13830.1481376748490506No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT13800.14781633499037589No Hit
GTCTTCGGAGGTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCC13750.14728076855925132No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT13670.14642386226945203No Hit
GGGTGCTGTCATACGTCTGGCAGTAATAATCAGCCTCATCCTCAGCCTGG13640.1461025224107773No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG13620.1458882958383275No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13310.14256778396535527No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC13260.14203221753423073No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGTCAGACTTGGAGCC13220.1416037643893311No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC12920.1383903658025838No Hit
ACAGCACCCTGCGTGGTTCTGTCTTCGGAGGTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCA12650.13549830707451122No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG12640.13539119378828632No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG12640.13539119378828632No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12530.13421294763981229No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG12420.13303470149133828No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG12240.1311066623392899No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC12180.13046398262194042No Hit
CCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGACGTATGACAGCA12150.13014264276326573No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11670.12500120502447004No Hit
GTTTGGGGGCTGTTCCTGGAAGTTGCTGGTACCAGTGTACATCATAATTT11650.1247869784520202No Hit
GTGTCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGATT11620.12446563859334547No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG11330.12135935329282309No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG11290.12093090014792345No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTG11290.12093090014792345No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA11270.12071667357547362No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA11260.12060956028924871No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT11210.12007399385812417No Hit
GAACCACGCAGGGTGCTGTCATACGTCTGGCAGTAATAATCAGCCTCATC11050.1183601812785256No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA10900.11675348198515195No Hit
GCGTGGTTCTGTCTTCGGAGGTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACT10690.11450410297442885No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG10620.11375430997085448No Hit
CTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC10590.11343297011217975No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10580.11332585682595483No Hit
CCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGG10530.11279029039483028No Hit
TCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGTCAGACTTGGAGCCAGAGAATCGGTCAGGGACC10470.11214761067748083No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10460.1120404973912559No Hit
GTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCT10430.11171915753258117No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG10270.11000534495298263No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCT10220.10946977852185806No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG10210.10936266523563316No Hit
CCCCTGAGGGCCGATTGGTGTTACCATAGATGAGGAGTTTGGGGGCTGTTCCTGGAAGTTGCTGGTACCAGTGTA10170.10893421209073352No Hit
CTCCTCATCTATGGTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATT10170.10893421209073352No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC10100.10818441908715916No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG10020.10732751279735987No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA9910.10614926664888587No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT9850.1055065869315364No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGACACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGG9820.10518524707286167No Hit
CACTGGTACCAGCAACTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACACCAATCGGCCCTCAGGGGTC9820.10518524707286167No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC9680.10368566106571293No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG9680.10368566106571293No Hit
CCTGAGGGCCGATTGGTGTTACCATAGATGAGGAGTTTGGGGGCTGTTCC9620.10304298134836347No Hit
GGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC9610.10293586806213856No Hit
GGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAAATTATGATGTACACTGGTACCAGCAACTTC9580.10261452820346383No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT9560.10240030163101402No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC9530.10207896177233929No Hit
GCCCAGTGATGGCCAGGGAGGCTGAGGTGTCAGACTTGGAGCCAGAGAAT9520.10197184848611436No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC9480.10154339534121472No Hit
CCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATC9410.10079360233764036No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTTG200.00558493955.4867964
GGACCGT200.008125070550.4690176
ATCTCGT2400.048.4174137
TCTCGTA2450.047.53536238
ATGCCGT2500.047.320244
TACGTAT606.1018585E-446.31025370
CGTATGC2550.046.0403641
CCGGCAA307.145978E-444.8541420
TCGTATG2700.044.6093940
AGGGGGG2650.044.562770
CACGAGA2750.044.03861621
ACGAGAC2800.043.25684422
ATCTCCG2750.042.8153210
GTATGCC2750.042.80965442
CCGTCTT2800.042.55430647
CGAGACT2850.042.50022523
GAGACTA2950.042.2113924
GGTATCA8400.042.0642781
CTTATAC3000.041.503421
TATACAC3000.041.496753
Produced by FastQC (version 0.11.5)