FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004688842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933591
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC44330.47483319783502625No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAAATTATGATGTACACTGGTACCAGCAACTTCCAGGAACAGC40820.43723643437008286No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT40760.4365937546527334No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAC37560.4023175030607622No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG36270.38849988913774874No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG34170.3660060990305177No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC33850.36257847387132053No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33490.35872239556722374No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC33480.35861528228099887No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32290.34586880122023456No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC31030.3323725271558959No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC25970.27817320432609144No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG25730.2756024854566936No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCACCTCCGAAGACAGAACCACGCAGGGTGCTGTCATACGT25170.2696041414280986No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24970.2674618757036004No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24890.26660496941380113No Hit
GACCTAGGACGGTGACCTTGGTCCCACCTCCGAAGACAGAACCACGCAGG23890.2558936407913101No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT22810.24432540587901982No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT22810.24432540587901982No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC22280.2386484017090996No Hit
ACCTAGGACGGTGACCTTGGTCCCACCTCCGAAGACAGAACCACGCAGGG22190.23768438213307538No Hit
GATTATTACTGCCAGACGTATGACAGCACCCTGCGTGGTTCTGTCTTCGG22140.23714881570195087No Hit
GTCATACGTCTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG21340.22857975280395804No Hit
ATGGTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGACACCTCAGCCTCCCTGG21060.22558058078966056No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC20460.21915378361616597No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG20370.21818976404014176No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG20320.21765419760901725No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC20120.21551193188451903No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG19470.2085495682798999No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGACGTATGACAGCACCCTGCGTGGTT19410.20790688856255043No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAAATTATGATGTACACTGGTACCAG19360.20737132213142587No Hit
TGACAGCACCCTGCGTGGTTCTGTCTTCGGAGGTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCC18130.19419638792576194No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG17860.19130432919768936No Hit
GTTCTGTCTTCGGAGGTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCC17620.1887336103282915No Hit
ACGTATGACAGCACCCTGCGTGGTTCTGTCTTCGGAGGTGGGACCAAGGT17600.1885193837558417No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA17590.18841227046961678No Hit
GTGTTACCATAGATGAGGAGTTTGGGGGCTGTTCCTGGAAGTTGCTGGTA17160.18380639916194566No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC17150.18369928587572074No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT16830.18027166071652362No Hit
GTGCTGTCATACGTCTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAG16570.17748671527467597No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC16290.17448754326037846No Hit
GGGTGCTGTCATACGTCTGGCAGTAATAATCAGCCTCATCCTCAGCCTGG16220.1737377502568041No Hit
GGATGAGGCTGATTATTACTGCCAGACGTATGACAGCACCCTGCGTGGTTCTGTCTTCGGAGGTGGGACCAAGGT16170.17320218382567956No Hit
GTCTGACACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATG15820.1694532188078077No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC15720.16838208594555862No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT15710.1682749726593337No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15330.1642046677827871No Hit
GATGTACACTGGTACCAGCAACTTCCAGGAACAGCCCCCAAACTCCTCAT15310.1639904412103373No Hit
GTGATGGCCAGGGAGGCTGAGGTGTCAGACTTGGAGCCAGAGAATCGGTC15260.16345487477921275No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG15070.16141972234093943No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG15040.1610983824822647No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC14700.15745653075061777No Hit
TGGTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGACACCTCAGCCTCCCTGGC14410.15435024545009537No Hit
GCCCCCAAACTCCTCATCTATGGTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT14340.153600452446521No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC14260.15274354615672173No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGACGTAT14000.14995860071487407No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA13990.14985148742864915No Hit
GGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGC13960.14953014756997443No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGACACCTCAG13780.14760210841792604No Hit
GCTCCAACATCGGGGCAAATTATGATGTACACTGGTACCAGCAACTTCCA13660.14631674898322714No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA13600.14567406926587767No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT13590.14556695597965277No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG13540.1450313895485282No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC13500.14460293640362856No Hit
GTCTTCGGAGGTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCC13470.14428159654495384No Hit
GCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGACGTATGACAGCA13280.14224644410668055No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT13140.1407468580995318No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12900.13817613923013397No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC12860.13774768608523433No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC12530.13421294763981229No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGTCAGACTTGGAGCC12400.13282047491888846No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG12390.13271336163266353No Hit
ACAGCACCCTGCGTGGTTCTGTCTTCGGAGGTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCA12150.13014264276326573No Hit
AGACAGAACCACGCAGGGTGCTGTCATACGTCTGGCAGTAATAATCAGCC12020.12875017004234188No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG12000.12853594346989206No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG11960.1281074903249924No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC11930.1277861504663177No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG11830.12671501760406859No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT11650.1247869784520202No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11610.12435852530712056No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG11590.12414429873467075No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA11580.12403718544844583No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG11550.1237158455897711No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA11520.1233945057310964No Hit
GTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG11430.12243048615507218No Hit
CCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGACGTATGACAGCA11240.1203953337167989No Hit
GTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCT11180.11975265399944944No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCT11070.11857440785097544No Hit
GCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGTC10920.11696770855760177No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10890.11664636869892706No Hit
GCGTGGTTCTGTCTTCGGAGGTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACT10860.11632502884025232No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10770.11536100926422813No Hit
GTGTCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGATT10590.11343297011217975No Hit
GGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTC10570.11321874353972991No Hit
GGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAAATTATGATGTACACTGGTACCAGCAACTTC10420.11161204424635628No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG10360.1109693645290068No Hit
CCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGG10350.1108622512427819No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10270.11000534495298263No Hit
GGCTGAGGATGAGGCTGATTATTACTGCCAGACGTATGACAGCACCCTGCGTGGTTCTGTCTTCGGAGGTGGGAC10230.10957689180808299No Hit
CTCCTCATCTATGGTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATT10190.10914843866318336No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG10110.10829153237338406No Hit
GTTTGGGGGCTGTTCCTGGAAGTTGCTGGTACCAGTGTACATCATAATTT10110.10829153237338406No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC9960.10668483308001041No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA9910.10614926664888587No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG9830.10529236035908658No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTG9830.10529236035908658No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG9740.1043283407830624No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA9720.10411411421061256No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT9690.10379277435193783No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC9660.1034714344932631No Hit
CTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC9560.10240030163101402No Hit
GGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC9460.10132916876876491No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)