FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111028
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT76896.925280109521922No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC14551.3104802392189359No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG7220.6502864142378499No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT3830.34495802860539687TruSeq Adapter, Index 1 (95% over 21bp)
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC3720.3350506178621609No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATGTCGTATGCCGT3520.3170371437835501No Hit
GCCTTGGGCTGACCCAGGACGGTCAACTTGGTCCCTCCGCCGAAGACCCGGTTGTTGCTGCCTGCATATGAGCTG2640.2377778578376626No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG2460.22156573116691286No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC2440.21976438375905177No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT2390.21526101523939908No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG2360.21255899412760745No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG2340.21075764671974637No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA2210.19904888856864936No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT2010.18103541449003854No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT1960.17653204597038585RNA PCR Primer, Index 1 (95% over 23bp)
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG1930.17383002485859422No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG1900.1711280037468026No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC1850.1666246352271499No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC1830.16482328781928884No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT1830.16482328781928884No Hit
GTACCAACAGTATCCAGGCAAAGCCCCCAAACTCATGATTTATGAAGTTA1810.16302194041142776No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT1790.16122059300356667No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1730.15581655077998344No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG1710.15401520337212235No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAACTTGGTCCCTC1660.14951183485246963No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA1650.1486111611485391No Hit
GAGACAGCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC1630.14680981374067803No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC1620.1459091400367475No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC1590.14320711892495588No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG1570.1414057715170948No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG1550.1396044241092337No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT1530.13780307670137262No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC1410.12699499225420616No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC1390.12519364484634504No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGAGCTCGTATGCCGT1380.12429297114241453No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAACTATGTCTCCTGGTACCAACAGTATCCAGGCAAAGC1380.12429297114241453No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT1360.12249162373455344No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1350.1215909500306229No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC1340.12069027632669235No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC1340.12069027632669235No Hit
TCTCTGGGCTCCAGGCTGAAGATGAGGCTTATTATTACTGCAGCTCATAT1340.12069027632669235No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATA1330.11978960262276184No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATATCGTATGCCGT1320.1188889289188313No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA1290.11618690780703965No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA1240.11168353928738696No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA1220.10988219187952589No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC1220.10988219187952589No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA1210.10898151817559534No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1210.10898151817559534No Hit
CTGTTGGTACCAGGAGACATAGTTATAAGCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGA1200.1080808444716648No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT1180.10627949706380373No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA1160.10447814965594264No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT1140.10267680224808155No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCAC1130.10177612854415104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG7200.0111.0489970
AAGGGGG7650.073.1417469
ATCGTAT208.148498E-569.55160539
ATGGGGG1550.068.2953869
ATATCGT150.002462680768.18516537
CTCTCGT150.002462680768.18516537
ATTGGGG309.6321455E-567.21133469
AATTGGG301.6392652E-460.37182268
AAAGGGG8450.059.4787468
GTATTGT200.004379656658.9573955
AAATTGG302.0196087E-457.87992567
AAAAGGG8550.056.86448767
AAAAAGG8550.055.8858766
CTTGAAA12500.053.55361657
TTGAAAA12700.053.22971758
TGAAAAA13100.052.4589559
GGCATGC253.4036726E-452.0919331
TGCTTGA12650.051.88871855
TATCGTA200.007325838351.77461238
GCTCTTA551.07320375E-1051.1647
Produced by FastQC (version 0.11.5)