FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111028
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1297711.688042655906617No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC3570.3215405123032028No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG2640.2377778578376626No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC2580.2323738156140793No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG2470.22246640487084338No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG2420.2179630363511907No Hit
GCCTTGGGCTGACCCAGGACGGTCAACTTGGTCCCTCCGCCGAAGACCCGGTTGTTGCTGCCTGCATATGAGCTG2400.2161616889433296No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT2200.19814821486471879No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG2180.1963468674568577No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT2160.19454552004899664No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA2140.19274417264113558No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG1910.17202867745073314No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT1900.1711280037468026No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAACTTGGTCCCTC1800.1621212667074972No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1750.1576178981878445No Hit
GTACCAACAGTATCCAGGCAAAGCCCCCAAACTCATGATTTATGAAGTTA1690.1522138559642613No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1640.14771048744460857No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1630.14680981374067803No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC1620.1459091400367475No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA1590.14320711892495588No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT1590.14320711892495588No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT1550.1396044241092337No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC1540.13870375040530317No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG1540.13870375040530317No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC1520.13690240299744208No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAACTATGTCTCCTGGTACCAACAGTATCCAGGCAAAGC1510.13600172929351154No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC1470.1323990344777894No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT1450.1305976870699283No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC1450.1305976870699283No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC1390.12519364484634504No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC1350.1215909500306229No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA1290.11618690780703965No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA1270.11438556039917859No Hit
TCTCTGGGCTCCAGGCTGAAGATGAGGCTTATTATTACTGCAGCTCATAT1240.11168353928738696No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT1240.11168353928738696No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG1230.11078286558345643No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG1220.10988219187952589No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC1220.10988219187952589No Hit
CTGTTGGTACCAGGAGACATAGTTATAAGCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGA1200.1080808444716648No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC1200.1080808444716648No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG1190.10718017076773426No Hit
CCCCTGAGGGCCGCTGACTAACTTCATAAATCATGAGTTTGGGGGCTTTGCCTGGATACTGTTGGTACCAGGAGA1190.10718017076773426No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT1170.10537882335987318No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT1170.10537882335987318No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATA1160.10447814965594264No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1160.10447814965594264No Hit
GCTTATAACTATGTCTCCTGGTACCAACAGTATCCAGGCAAAGCCCCCAAACTCATGATTTATGAAGTTAGTCAG1150.1035774759520121No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA1140.10267680224808155No Hit
GGCTTATTATTACTGCAGCTCATATGCAGGCAGCAACAACCGGGTCTTCGGCGGAGGGACCAAGTTGACCGTC1140.10267680224808155No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1120.1008754548402205No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAAATA150.001301727180.0617960
ACAGGCC201.3203188E-463.096491
CGGGTGA150.00336180963.039646
CTACACT200.00543517355.83032250
GGCATAA200.005573863655.4762249
GCTACAC200.005573863655.4762249
TTAATCA200.006167697354.07573344
CGGGACC301.5449386E-552.55670517
CCCTATC250.00857598849.7445564
CCTAGAA250.00888138649.306463
CCTAACG456.060145E-849.0750432
CTAACGC456.060145E-849.0750433
ACCTAAC456.060145E-849.0750431
TCCTAGA250.0092797548.76236762
TAACGCC552.94478E-740.1342124
ACGCCAT552.9552393E-740.116146
AACGCCA552.9552393E-740.116145
ACGGGGT907.2759576E-1238.52422316
ACAGGTA350.00208322236.0551342
ACCACTG350.002092465836.022659
Produced by FastQC (version 0.11.5)