FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2075057
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGTCAGCCTCATCCTCAGACTGGAGGCCAGAGATGAGTAAAATCC81550.39300125249571455No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC71310.3436532104901215No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT68160.32847290459972905No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGTCAC65410.3152202566001801No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG65260.31449738489111384No Hit
GACTATTACTGTATGATTTGGCGCGGCACCGCTGTGACATTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAG64180.30929270858583646No Hit
CACCTGCACCTTACGCAGTGACATCAATGTTGGTACCTCCACGATATACTGGTACCAGCAGAATCCAGGCAGTC60560.29184740467370296No Hit
GTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAG55880.2692938073508342No Hit
GATATGCACCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGGATTTTA55460.26726976656544854No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT53000.25541467053676115No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC52220.25165573764961635No Hit
GGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAGTCTCAC45390.21874097916346394No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC44920.21647598114172287No Hit
GGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCC44860.2161868324580963No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA44810.21594587522174089No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT44810.21594587522174089No Hit
GTGGTAAACAGCCGTGTCGTCGGCTCTCAGGCTGTCCATTTGCAGAGACA44250.21324715417456003No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGTCACAGCGGTGCCGCGCCAAATCATACAGTAATAGTCAG43530.20977736997104174No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG43200.2081870522110959No Hit
CCCCAGTATCTCCTGAGGTACAAATCAGACTCGGATATGCACCAGGGCTC43060.20751237194930067No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG42620.20539194826937282No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG41940.2021149298549389No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG41230.19869333709869175No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA40580.1955608930260711No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGGCCAGAGATGAGTAAAAT39570.19069355685169131No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC38630.18616356080820914No Hit
CTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAG38580.1859226035718537No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC38500.185537071993685No Hit
GCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGGATTTTACTCATCTCTGGC38470.18539249765187174No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG37600.18119984173928716No Hit
GTCCTCCCCAGTATCTCCTGAGGTACAAATCAGACTCGGATATGCACCAGGGCTCTGGAGTCCCCAGCCGCTTCT37530.18086250160838957No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG37020.17840473779756413No Hit
GTGCATATCCGAGTCTGATTTGTACCTCAGGAGATACTGGGGAGGACTGCCTGGATTCTGCTGGTACCAGTATAT36610.17642888845944954No Hit
ATATACTGGTACCAGCAGAATCCAGGCAGTCCTCCCCAGTATCTCCTGAGGTACAAATCAGACTCGGATATGCAC36580.1762843141176363No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG36350.17517591083040127No Hit
TCCTCAGACTGGAGGCCAGAGATGAGTAAAATCCCTGCATTGGCCGAAGC36170.17430846477952172No Hit
CTCCAGAGCCCTGGTGCATATCCGAGTCTGATTTGTACCTCAGGAGATACTGGGGAGGACTGCCTGGATTCTG36020.1735855930704554No Hit
CTATTACTGTATGATTTGGCGCGGCACCGCTGTGACATTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCC35770.17238080688867824No Hit
GGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCT35340.1703085746560215No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA35210.16968208584149735No Hit
GTACGGGACTGTGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCA35100.16915197992151543No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT34910.16823634242336477No Hit
TCTCTGGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTATGATTTGG34490.1662123016379791No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC33770.16274251743446086No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT33620.16201964572539454No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGTCACAG33270.16033294507090648No Hit
CCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCT33210.16004379638727997No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA32880.1584534786273341No Hit
GCTTGGTCCCTCCGCCGAATGTCACAGCGGTGCCGCGCCAAATCATACAGTAATAGTCAGCCTCATCCTCAGA32840.15826071283824975No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG31850.15348975955841213No Hit
GTTTACCACTGTGCGAGAGATCGGGGGCAGATGGCCCCAATTGACCACTGGGGCCAGGGAACCCTGGTCACCGT31790.1532006108747856No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA31700.15276688784934583No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC30560.1472730628604419No Hit
CATCAATGTTGGTACCTCCACGATATACTGGTACCAGCAGAATCCAGGCAGTCCTCCCCAGTATCTCCTGAGGTA30490.1469357227295443No Hit
GTCTGAGGATGAGGCTGACTATTACTGTATGATTTGGCGCGGCACCGCTGTGACATTCGGCGGAGGGACCAAG30020.1446707247078032No Hit
GTACAAATCAGACTCGGATATGCACCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGC30020.1446707247078032No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC29330.1413455148460982No Hit
CACCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGGATTTTACTCAT28800.1387913681407306No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGTCACAGCGGTGC28690.13826126222074864No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT27630.1331529688100134No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT27470.13238190565367602No Hit
ATACTGGGGAGGACTGCCTGGATTCTGCTGGTACCAGTATATCGTGGAGG27370.13189999118096513No Hit
CTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAG27350.13180360828642296No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG27040.1303096734210193No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG26840.12934584447559755No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC26680.12857478131926015No Hit
GGGCTAGAGTGGGTGGCAGTCATATGGCATGATGGAACTAAAAAATACTA26490.1276591438211095No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT26320.126839889217501No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC26290.12669531487568775No Hit
ATGCAGGGATTTTACTCATCTCTGGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTATGATTTGGCGCGGCA26180.1261652089557058No Hit
GATTTGTACCTCAGGAGATACTGGGGAGGACTGCCTGGATTCTGCTGGTA26150.12602063461389254No Hit
GCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAGT26150.12602063461389254No Hit
ATGTTGGTACCTCCACGATATACTGGTACCAGCAGAATCCAGGCAGTCCTCCCCAGTATCTCCTGAGGTACAAAT25990.12524957145755514No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC25790.1242857425121334No Hit
CTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCT25600.12337010501398274No Hit
CTCCTGGAGCATCAGCCAGTCTCACCTGCACCTTACGCAGTGACATCAAT25390.12235808462128991No Hit
GCCTCATCCTCAGACTGGAGGCCAGAGATGAGTAAAATCCCTGCATTGGCCGAAGCATCTTTGGATCCAGAGAAG25320.12202074449039232No Hit
GTCTCACCTGCACCTTACGCAGTGACATCAATGTTGGTACCTCCACGATA25290.12187617014857906No Hit
GGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG25230.12158702146495254No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC25190.12139425567586819No Hit
GGGGAGGACTGCCTGGATTCTGCTGGTACCAGTATATCGTGGAGGTACCA25030.12062319251953078No Hit
ACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGTCACAGCGGTGCCGCGCCAAATCATACAGTAATAGTCAGC24810.11956298067956689No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC24560.11835819449778968No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT24150.11638234515967513No Hit
GTGCAGGTGAGACTGGCTGATGCTCCAGGAGATGCAGAGAGGGAAGACGG24150.11638234515967513No Hit
GTATCTCCTGAGGTACAAATCAGACTCGGATATGCACCAGGGCTCTGGAG23680.11411734713793403No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG23520.11334628398159666No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA23510.11329809253432556No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA22730.10953915964718078No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC22580.10881628793811447No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT22550.10867171359630122No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG22460.10823799057086143No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG22220.10708139583635533No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC22130.10664767281091557No Hit
ATACTGGTACCAGCAGAATCCAGGCAGTCCTCCCCAGTATCTCCTGAGGT21870.1053946951818673No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC21850.10529831228732513No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG21660.10438267478917447No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG21640.1042862918946323No Hit
CCAGTATATCGTGGAGGTACCAACATTGATGTCACTGCGTAAGGTGCAGG21250.10240682545105989No Hit
GATTTTACTCATCTCTGGCCTCCAGTCTGAGGATGAGGCTGACTATTACT21200.10216586821470447No Hit
GGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGGATTTTACTCATCTCTGG21000.10120203926928273No Hit
CTGCATCTCCTGGAGCATCAGCCAGTCTCACCTGCACCTTACGCAGTGAC20930.10086469913838511No Hit
CTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCC20880.10062374190202969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2350.063.32683670
GGTATCA15750.046.7276841
GTGGTAT4200.045.6409951
ATCTCGT2700.043.3635737
TATCTCG2800.043.01030336
TGGTATC4550.042.1240582
TCGTATG2300.040.5445540
CCGTTAA603.1031414E-739.92528516
TCTCGTA2750.038.85525538
ACCGATC4150.038.7860527
TATTCCG459.797952E-538.47844743
TCAGTAT4300.038.31265332
CGATCAG4250.037.88897329
GACAGAC1200.037.083311
ACGAGAC4450.036.91504722
GCCGTTA655.8101796E-736.85499615
CTTATAC4500.036.5127941
TATACAC4500.036.5083963
GACCGAT4450.036.15905426
TACACAT4600.035.7147335
Produced by FastQC (version 0.11.5)