FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2075057
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGTCAGCCTCATCCTCAGACTGGAGGCCAGAGATGAGTAAAATCC74520.35912266506414037No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG67790.3266898210506988No Hit
GACTATTACTGTATGATTTGGCGCGGCACCGCTGTGACATTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAG64630.31146132371303537No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC64280.3097746230585473No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT64040.3086180283240412No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59990.289100492179251No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGTCAC59220.2853897507393773No Hit
GTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAG57540.2772935875978347No Hit
CACCTGCACCTTACGCAGTGACATCAATGTTGGTACCTCCACGATATACTGGTACCAGCAGAATCCAGGCAGTC54530.2627879619692375No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC50870.24514989226801964No Hit
GATATGCACCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGGATTTTA49840.24018617319909763No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT48450.23348756202841658No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG46400.22360831533784373No Hit
GGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCC45990.22163246599972916No Hit
GGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAGTCTCAC45590.2197048081088857No Hit
GTGGTAAACAGCCGTGTCGTCGGCTCTCAGGCTGTCCATTTGCAGAGACA44820.21599406666901197No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT44270.2133435370691022No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG43580.21001832720739722No Hit
CTCCAGAGCCCTGGTGCATATCCGAGTCTGATTTGTACCTCAGGAGATACTGGGGAGGACTGCCTGGATTCTG43300.20866896668380674No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGTCACAGCGGTGCCGCGCCAAATCATACAGTAATAGTCAG41770.20129567525133044No Hit
CCCCAGTATCTCCTGAGGTACAAATCAGACTCGGATATGCACCAGGGCTC41100.1980668482841676No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA40570.1955127015788No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC40530.19531993578971565No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG39590.19078993974623348No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG39220.18900685619720325No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA38630.18616356080820914No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC38450.18529611475732954No Hit
GTCCTCCCCAGTATCTCCTGAGGTACAAATCAGACTCGGATATGCACCAGGGCTCTGGAGTCCCCAGCCGCTTCT38140.18380217989192588No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC37870.1825010108156065No Hit
CTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAG37650.1814407989756426No Hit
ATATACTGGTACCAGCAGAATCCAGGCAGTCCTCCCCAGTATCTCCTGAGGTACAAATCAGACTCGGATATGCAC37520.18081431016111846No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG37270.1796095239793413No Hit
GCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGGATTTTACTCATCTCTGGC36220.17454942201587717No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGGCCAGAGATGAGTAAAAT35900.17300729570320236No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG35780.17242899833594932No Hit
TCCTCAGACTGGAGGCCAGAGATGAGTAAAATCCCTGCATTGGCCGAAGC35660.17185070096869628No Hit
CTATTACTGTATGATTTGGCGCGGCACCGCTGTGACATTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCC35560.17136878649598541No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG35360.17040495755056367No Hit
GTACGGGACTGTGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCA35090.16910378847424432No Hit
GGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCT34850.16794719373973824No Hit
CCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCT34390.16573038716526822No Hit
GTGCATATCCGAGTCTGATTTGTACCTCAGGAGATACTGGGGAGGACTGCCTGGATTCTGCTGGTACCAGTATAT34090.1642846437471356No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGTCACAG32390.15609209771105084No Hit
TCTCTGGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTATGATTTGG32260.1554656088965267No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA32230.15532103455471344No Hit
GTCTGAGGATGAGGCTGACTATTACTGTATGATTTGGCGCGGCACCGCTGTGACATTCGGCGGAGGGACCAAG32110.1547427371874604No Hit
GCTTGGTCCCTCCGCCGAATGTCACAGCGGTGCCGCGCCAAATCATACAGTAATAGTCAGCCTCATCCTCAGA31450.15156210166756864No Hit
GTTTACCACTGTGCGAGAGATCGGGGGCAGATGGCCCCAATTGACCACTGGGGCCAGGGAACCCTGGTCACCGT31270.1506946556166891No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC30940.1491043378567432No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT30670.14780316878042385No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA30360.14630923391502015No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT29900.14409242734055017No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA29730.14327317273694168No Hit
CATCAATGTTGGTACCTCCACGATATACTGGTACCAGCAGAATCCAGGCAGTCCTCCCCAGTATCTCCTGAGGTA29620.14274306681695972No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC28620.13792392208985102No Hit
GGGCTAGAGTGGGTGGCAGTCATATGGCATGATGGAACTAAAAAATACTA28220.13599626419900754No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGTCACAGCGGTGC28100.1354179668317545No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC27950.1346950951226882No Hit
GTACAAATCAGACTCGGATATGCACCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGC27890.13440594643906167No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT27750.13373126617726644No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG27750.13373126617726644No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT27570.1328638201263869No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC27480.1324300971009471No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC27170.1309361622355434No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG26800.12915307868651318No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC26570.1280446753992782No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA26310.12679169777022994No Hit
CACCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGGATTTTACTCAT26290.12669531487568775No Hit
ATGTTGGTACCTCCACGATATACTGGTACCAGCAGAATCCAGGCAGTCCTCCCCAGTATCTCCTGAGGTACAAAT26280.12664712342841666No Hit
GCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAGT26000.12529776290482622No Hit
ATACTGGGGAGGACTGCCTGGATTCTGCTGGTACCAGTATATCGTGGAGG25790.1242857425121334No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT25770.12418935961759123No Hit
ATGCAGGGATTTTACTCATCTCTGGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTATGATTTGGCGCGGCA25750.12409297672304904No Hit
CTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCT25440.12259904185764536No Hit
GTATCTCCTGAGGTACAAATCAGACTCGGATATGCACCAGGGCTCTGGAG25400.12240627606856101No Hit
CTCTAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCGTAGTCAC25290.12187617014857906No Hit
GGGGAGGACTGCCTGGATTCTGCTGGTACCAGTATATCGTGGAGGTACCA25010.12052680962498862No Hit
CTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAG24970.12033404383590426No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC24660.11884010897050057No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC24340.11729798265782579No Hit
GTCTCACCTGCACCTTACGCAGTGACATCAATGTTGGTACCTCCACGATA24190.11657511094875948No Hit
GGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG24080.11604500502877751No Hit
GATTTGTACCTCAGGAGATACTGGGGAGGACTGCCTGGATTCTGCTGGTA24010.11570766489787992No Hit
GGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGGATTTTACTCATCTCTGG23970.11551489910879556No Hit
GCCTCATCCTCAGACTGGAGGCCAGAGATGAGTAAAATCCCTGCATTGGCCGAAGCATCTTTGGATCCAGAGAAG23700.11421373003247622No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG23210.11185234911619295No Hit
CTCCTGGAGCATCAGCCAGTCTCACCTGCACCTTACGCAGTGACATCAAT23040.11103309451258446No Hit
CTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCC22850.11011745701443382No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG22800.1098764997780784No Hit
GATTTTACTCATCTCTGGCCTCCAGTCTGAGGATGAGGCTGACTATTACT22720.1094909681999097No Hit
CTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCT22110.10655128991637337No Hit
GTGCAGGTGAGACTGGCTGATGCTCCAGGAGATGCAGAGAGGGAAGACGG21960.10582841820730708No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT21720.10467182347280099No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA21500.10361161163283707No Hit
ATACTGGTACCAGCAGAATCCAGGCAGTCCTCCCCAGTATCTCCTGAGGT21310.10269597413468641No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG21020.1012984221638249No Hit
ACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGTCACAGCGGTGCCGCGCCAAATCATACAGTAATAGTCAGC20820.10033459321840317No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAC200.0076321651.2766383
GACAGGT2800.046.4311941
GGTATCA18150.044.108631
GTGGTAT4500.040.2946781
TGGTATC5000.036.246852
GTATCAA39900.033.869481
GGATGGG15850.033.4459670
TATCAAC44300.030.490022
ATCAACG46200.029.2269443
GTGCTAG1650.029.0287551
TCAACGC46700.028.8387364
CAACGCA47250.028.5747015
TAGACTG605.726491E-428.4842765
GACAGGG4250.028.1749671
AACGCAG47950.028.1575536
GGGTCGA500.00806637427.36468926
ACGCAGA50800.026.5778477
GCATTCG658.7837985E-426.4737337
AGAGTAC51050.026.44896511
CGCAGAG51150.026.3953488
Produced by FastQC (version 0.11.5)