FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1626110
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTATATCTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTGAGAGTGTTCGCAACCAGTTAGG157160.9664782825270123No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT69140.4251864879989669No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC66690.41011985659026756No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA52920.3254392384279046No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC50590.311110564475958No Hit
GCGTTACACCTATCTCACCTTCGGCGGAGGGACCAGGGTGGCGATCAAACGAACTGTGGCTGCACCATCTGTCTT49450.3040999686368081No Hit
GTGTAACGCTGGTGGCAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTC45510.27987036547343047No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42150.25920755668435713No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC41640.2560712374931585No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT40390.2483841806519854No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTT40300.24783071255942093No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT39890.24530935791551617No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38440.23639237197975535No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA37150.22845932931966473No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT36420.22397008812441965No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG35980.2212642441163267No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG35420.21782044265148112No Hit
CTCCTGCAGGGCCAGTGAGAGTGTTCGCAACCAGTTAGGCTGGTATCAACAGAAACGTGGCCAGGCTCCCAGGCT35370.2175129603778342No Hit
GATATAGGGTGTCTGGCGACTGTGTCAACACAATTTCTCCGGTGGTATCT34070.20951842126301418No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG33660.2069970666191094No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG32700.20109340696508848No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT32410.1993100097779363No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGCCACCAGCGTTACACC32330.19881803814010124No Hit
GTAATAGACGGCCGTGTCTGTGGCGCTCACAGACGTCAGCCTCAGGGAGAACTGATTCTTCGCCATGTCCACTGA32040.19703464095294906No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCGCCACCCTGGTCC31420.1932218607597272No Hit
CAGTTAGGCTGGTATCAACAGAAACGTGGCCAGGCTCCCAGGCTCCTCATCTATGATTCATCCAACAGGGCCA30370.18676473301314178No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29380.1806765839949327No Hit
GTTTATTACTGCCACCAGCGTTACACCTATCTCACCTTCGGCGGAGGGAC29090.17889318680778052No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGCCACCAGCGTTACACCTAT28060.1725590519706539No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27760.17071415832877235No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA27530.1692997398699965No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25990.15982928584167125No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24930.15331066164035642No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA24760.1522652219099569No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24610.1513427750890161No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT24110.1482679523525469No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT24040.14783747716944118No Hit
GTCTATTACTGTGCGAGACGTGTTTTTGGAGACATTTTTGGGTCGGTGGG23520.14463966152351318No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22990.14138034942285577No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21440.13184839893980113No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC21390.1315409166661542No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21250.1306799662999428No Hit
GTGCAGCCACAGTTCGTTTGATCGCCACCCTGGTCCCTCCGCCGAAGGTG21040.12938854075062572No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20810.12797412229184987No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20600.1266826967425328No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20590.1266212002878034No Hit
GACCAGGGTGGCGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT20320.12496079601011002No Hit
GTGAGAGTGTTCGCAACCAGTTAGGCTGGTATCAACAGAAACGTGGCCAGGCTCCCAGGCTCCTCATCTATGATT20000.12299290945876971No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19960.12274692363985215No Hit
GTGTTGACACAGTCGCCAGACACCCTATATCTGTCTCCAGGGGAAAGAGC19950.12268542718512278No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19760.12151699454526446No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19230.11825768244460708No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19040.11708924980474876No Hit
CTCCCAGGCTCCTCATCTATGATTCATCCAACAGGGCCACTGGCATCCCA18980.11672027107637245No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18700.11499837034394968No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18590.11432190934192643No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18460.11352245543044444No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18450.11346095897571504No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT18370.11296898733787998No Hit
GCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGG18160.11167756178856289No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG18150.1116160653338335No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18020.1108166114223515No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTGAGAGTGTTCGCAACCAGTTAGGCTGGTATCAACAGAAACGTGGCCAGG17930.11026314332978704No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT17910.11014015042032828No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17830.1096481787824932No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA17690.1087872284162818No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17610.10829525677844673No Hit
CCCAGATACCACCGGAGAAATTGTGTTGACACAGTCGCCAGACACCCTATATCTGTCTCCAGGGGAAAGAGCCAC17540.10786478159534103No Hit
GCCTGGGAGCCTGGCCACGTTTCTGTTGATACCAGCCTAACTGGTTGCGA17090.1050974411325187No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC17040.10478995885887178No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17010.10460546949468362No Hit
ACCCTGGTCCCTCCGCCGAAGGTGAGATAGGTGTAACGCTGGTGGCAGTA16940.10417499431157792No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA16930.10411349785684855No Hit
CCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTCGC16900.1039290084926604No Hit
GGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGC16870.10374451912847224No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16790.10325254749063717No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16700.10269907939807271No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16560.10183812903186132No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC16410.10091568221092054No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16340.10048520702781484No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)