FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1626110
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTATATCTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTGAGAGTGTTCGCAACCAGTTAGG149440.9190030194759271No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT68560.42161969362466256No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC55430.34087484856498024No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA49660.3053913941861252No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC49420.30391547927261997No Hit
GCGTTACACCTATCTCACCTTCGGCGGAGGGACCAGGGTGGCGATCAAACGAACTGTGGCTGCACCATCTGTCTT48670.29930324516791607No Hit
GTGTAACGCTGGTGGCAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTC47400.2914931954172842No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43450.26720209579917714No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT40250.24752323028577403No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39430.24248052099796447No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC39350.2419885493601294No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT39180.24094310962972984No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38390.23608488970610844No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTT35320.21720547810418728No Hit
CTCCTGCAGGGCCAGTGAGAGTGTTCGCAACCAGTTAGGCTGGTATCAACAGAAACGTGGCCAGGCTCCCAGGCT35250.2167750029210816No Hit
GTAATAGACGGCCGTGTCTGTGGCGCTCACAGACGTCAGCCTCAGGGAGAACTGATTCTTCGCCATGTCCACTGA34110.2097644070819317No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33970.2089034567157203No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT33960.20884196026099097No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33670.2070585630738388No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG32380.19912552041374815No Hit
GATATAGGGTGTCTGGCGACTGTGTCAACACAATTTCTCCGGTGGTATCT30910.19008554156852858No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGCCACCAGCGTTACACC30170.1855348039185541No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30020.1846123570976133No Hit
GTTTATTACTGCCACCAGCGTTACACCTATCTCACCTTCGGCGGAGGGAC29270.18000012299290946No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29130.17913917262669807No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCGCCACCCTGGTCC28930.17790924353211038No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28750.17680230734698146No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28660.17624883925441698No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA28290.17397347042942976No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA28260.1737889810652416No Hit
CAGTTAGGCTGGTATCAACAGAAACGTGGCCAGGCTCCCAGGCTCCTCATCTATGATTCATCCAACAGGGCCA27900.1715751086949837No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGCCACCAGCGTTACACCTAT27690.17028368314566664No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT25810.1587223496565423No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25690.15798439219978969No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24590.15121978217955734No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA24250.14912890271875828No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT23340.14353272533838424No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC21490.13215588121344804No Hit
GTCTATTACTGTGCGAGACGTGTTTTTGGAGACATTTTTGGGTCGGTGGG21490.13215588121344804No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21420.13172540603034233No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21320.1311104414830485No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21280.13086445566413096No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20410.1255142641026745No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20210.12428433500808679No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20170.12403834918916924No Hit
GTGCAGCCACAGTTCGTTTGATCGCCACCCTGGTCCCTCCGCCGAAGGTG20140.1238538598249811No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19850.12207046263782893No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19820.12188597327364077No Hit
GTGTTGACACAGTCGCCAGACACCCTATATCTGTCTCCAGGGGAAAGAGC19370.11911863281081846No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19240.11831917889933645No Hit
GTGAGAGTGTTCGCAACCAGTTAGGCTGGTATCAACAGAAACGTGGCCAGGCTCCCAGGCTCCTCATCTATGATT19160.11782720726150138No Hit
GACCAGGGTGGCGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT19130.11764271789731322No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18470.11358395188517383No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18310.11260000860950366No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18090.1112470866054572No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18030.11087810787708087No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17870.10989416460141072No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA17830.1096481787824932No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17820.10958668232776382No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17800.10946368941830503No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17790.10940219296357566No Hit
GCCTGGGAGCCTGGCCACGTTTCTGTTGATACCAGCCTAACTGGTTGCGA17750.10915620714465812No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17470.10743430641223534No Hit
CTCCCAGGCTCCTCATCTATGATTCATCCAACAGGGCCACTGGCATCCCA17470.10743430641223534No Hit
GGTGTAACGCTGGTGGCAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGT17290.1063273702271064No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17190.10571240567981256No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16990.10448247658522486No Hit
GCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGG16960.10429798722103671No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16560.10183812903186132No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC16490.10140765384875562No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16370.100669696392003No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)