FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004688851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2029471
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCGGCTGTCAGACTGGTCATTTGCAGGTACA89320.44011468998571546No Hit
CTATATGTCTTCGGAAGTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTC73020.35979819371649063No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT61490.30298535923893466No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCA60110.29618555771430094No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC57990.28573948580689257No Hit
GACATATAGAGTGCTGCTGCTTCTATGTGAGCAGCAGTAATAATCAGCCT54280.2674588599689279No Hit
GTCTATTACTGTGCAAGAGCTGGGAACTTCCGCTTTGACTTCTGGGGCCA53750.26484734199207577No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCGATCACCATCTCCTG52630.2593286624938223No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC49740.24508849843136463No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC49360.24321608931588576No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC49290.2428711718472449No Hit
GATTATTACTGCTGCTCACATAGAAGCAGCAGCACTCTATATGTCTTCGG49060.2417378715931393No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG48080.2369090270321675No Hit
CTCTATATGTCTTCGGAAGTGGGACCAAGGTCACCGTCCTAGGTCAGCCC47100.23208018247119572No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG46290.22808899461978024No Hit
CATATAGAGTGCTGCTGCTTCTATGTGAGCAGCAGTAATAATCAGCCTCG42780.21079384726364653No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC39990.19704642244210438No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC39000.19216830395704104No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC38210.18827566395380865No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT37330.18393955863375233No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT36890.18177150597372418No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACATATAGAGTGCTGCTGCTTCTATGTGAG36150.17812523559094956No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT35820.17649919609592843No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG35740.17610500470319604No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTTATTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC35590.17536589584182283No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCGATCAC34740.17117761229404116No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA34240.1687139160894637No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCACAT34140.1682211768485482No Hit
GACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACATATAGAGTGCTG33810.1665951373535271No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG31650.1559519697497525No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG31620.15580414797747788No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCGATCACCATCT31370.15457229987518917No Hit
CATAGAAGCAGCAGCACTCTATATGTCTTCGGAAGTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAAC31290.15417810848245675No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACATATAGAGTG31150.15348827354517508No Hit
ACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACATATAGAGTGCTGC31000.15274916468380184No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG29150.14363348872686527No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT28610.14097269682592165No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG28200.13895246593816812No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG28150.13870609631771036No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA28020.13806553530452023No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC27610.13604530441676674No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG27260.13432071707356252No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC26340.12978751605714No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC25830.127274545928471No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT25780.12702817630801327No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC24890.12264279706386541No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGTTCCTGGATGCACTGGGTCCGCCAAGCTCCAGGGGAGGAG24670.12155877073385132No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT24460.1205240183279288No Hit
CTCCTGGGCAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATT24250.11948926592200627No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT24220.11934144414973162No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTTATTATAACTATGTCTCCTGGTACCAACAGCA24020.11835596566790066No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC23660.11658210440060489No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG23450.11554735199468236No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA23420.1153995302224077No Hit
GCTTCTATGTGAGCAGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCC22570.11121124667462605No Hit
CCCCTGAGGGCCGACTACTGACATCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA22210.10943738540733028No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCGATCAC22090.1088460983182317No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC21900.10790989376049226No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA21820.10751570236775987No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC21350.10519982793545707No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT21150.10421434945362608No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC21130.10411580160544298No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT21110.10401725375725988No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG20930.10313032312361202No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT20860.10278540565497117No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT20830.10263758388269653No Hit
GACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCT20770.10234194033814724No Hit
GGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC20740.10219411856587257No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG20600.1015042836285909No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)