FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2029471
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCGGCTGTCAGACTGGTCATTTGCAGGTACA78580.3871944955113919No Hit
CTATATGTCTTCGGAAGTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTC68480.3374278321789274No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC64740.3189993845686881No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64160.3161414969713782No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT57950.28554239011052635No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCA57150.2816004761832024No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC53320.2627285632561392No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCGATCACCATCTCCTG51890.25568239211104765No Hit
GTCTATTACTGTGCAAGAGCTGGGAACTTCCGCTTTGACTTCTGGGGCCA51320.2528737784378294No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC50710.24986806906824488No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG48050.23676120525989286No Hit
GACATATAGAGTGCTGCTGCTTCTATGTGAGCAGCAGTAATAATCAGCCT47770.23538153538532947No Hit
GATTATTACTGCTGCTCACATAGAAGCAGCAGCACTCTATATGTCTTCGG45580.22459054600928027No Hit
CTCTATATGTCTTCGGAAGTGGGACCAAGGTCACCGTCCTAGGTCAGCCC44870.22109209739878027No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG44820.22084572777832254No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC44470.2191211404351183No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC40330.19872173586121702No Hit
CATATAGAGTGCTGCTGCTTCTATGTGAGCAGCAGTAATAATCAGCCTCG39530.19477982193389312No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT37490.18472794141921714No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA36840.18152513635326645No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCGATCAC36170.17822378343913264No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC35820.17649919609592843No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACATATAGAGTGCTGCTGCTTCTATGTGAG35020.17255728216860453No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT34480.16989649026766088No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT34470.16984721634356933No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG34350.16925592925447075No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC33570.16541256317532993No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTTATTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC33250.16383579760440037No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCACAT33110.16314596266711867No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG32440.1598446097529849No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC31020.15284771253198495No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG30290.14925071607330187No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACATATAGAGTG30190.14875797683238637No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCGATCACCATCT29250.14412622796778077No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA28950.14264801024503432No Hit
CATAGAAGCAGCAGCACTCTATATGTCTTCGGAAGTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAAC28850.14215527100411882No Hit
GACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACATATAGAGTGCTG28700.14141616214274558No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG28190.13890319201407655No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC27610.13604530441676674No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG27290.13446853884583715No Hit
ACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACATATAGAGTGCTGC27080.13343378643991463No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG25710.12668325883937243No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG25180.12407174086252032No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGTTCCTGGATGCACTGGGTCCGCCAAGCTCCAGGGGAGGAG25170.12402246693842879No Hit
CTCCTGGGCAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATT24970.1230369884565978No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT24660.12150949680975978No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTTATTATAACTATGTCTCCTGGTACCAACAGCA24590.12116457934111895No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC23630.11643428262833025No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC23580.11618791300787248No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT23500.1157937216151401No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC22850.11259091654918943No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC22770.11219672515645704No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT22520.1109648770541683No Hit
CCCCTGAGGGCCGACTACTGACATCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA22390.11032431604097817No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCGATCAC21910.10795916768458383No Hit
GCTTCTATGTGAGCAGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCC21800.10741715451957677No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC21800.10741715451957677No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG21560.10623458034137959No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC21560.10623458034137959No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA21380.10534764970773172No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC21240.10465781477045004No Hit
GGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC20880.10288395350315427No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG20870.10283467957906273No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA20770.10234194033814724No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC20700.10199702286950638No Hit
GACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCT20610.10155355755268244No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT20520.10111009223585851No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGAG27000.045.81122270
TCGTCAG18150.043.81002470
GGTATCA16300.042.4042471
GTGGTAT4200.042.1555751
TGGTATC4550.038.8917352
TCGGCTG20400.035.03288320
GTATCAA36700.034.6981621
TTGCAGG21200.034.31549540
CTCGGCT20850.034.2767819
GCAGGTA21550.033.8630242
GGTCATT21500.033.6017234
ATTTGCA21700.033.26789538
TTTGCAG22100.032.8704239
ACTGGTC21750.032.8184531
TCATTTG22100.032.57815636
GTCATTT22300.032.26434335
TGCAGGT22650.032.01844441
CCTCGGC22700.031.63087818
CTGTCAG22600.031.62572324
GGTCTAT652.5548043E-531.4048143
Produced by FastQC (version 0.11.5)