FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866018
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC42050.4855557274791055No Hit
GCCTTGGGCTGACCCAGGACGGTCAACTTGGTCCCTCCGCCGAAAAGCAC39740.4588819170040346No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTC29710.3430644628633585No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAACTTGGTCCCTC25030.28902401566711083No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24420.2819802821650358No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23540.271818830555485No Hit
CTCCTGGACAGTCGATCACCATCTCCTGTATTGGATCAAGTAGTGACGTT21130.2439903096702378No Hit
GTCTGGAACTGAGGTGAAGAACCCTGGGGCCTCAGTGAAGGTCTCCTGTA19520.22539947206640046No Hit
GTATTGGATCAAGTAGTGACGTTGGTACTTCTAAGTATGTCTCCTGGTAC18510.21373689692362052No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18460.2131595417185324No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17690.2042682715601754No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC17210.19872566159132948No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17110.19757095118115328No Hit
GTCGATCACCATCTCCTGTATTGGATCAAGTAGTGACGTTGGTACTTCTAAGTATGTCTCCTGGTACCAACAGCA16610.19179739913027213No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16290.1881023258177082No Hit
CTCCTGTATTGGATCAAGTAGTGACGTTGGTACTTCTAAGTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGT15960.1842917814641266No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15800.18244424480784463No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15290.17655522171594584No Hit
TCTCTGGGCTCCAACCTGAGGACGAGGCTGATTATTATTGCAGCTCATAT15230.17586239546984012No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15060.17389938777254052No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14900.17205185111625854No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14420.16650924114741264No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14260.16466170449113066No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG14250.16454623345011304No Hit
CCCCTGGACAAGGGCTTGAGTGGATAGGATGGATCAGCGCTTACAATGGA14140.1632760519989192No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13990.16154398638365486No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13940.16096663117856672No Hit
GACCCAGGACGGTCAACTTGGTCCCTCCGCCGAAAAGCACGGTGGTTCTG13820.15958097868635526No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG13700.15819532619414375No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13430.15507760808666796No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC13410.1548466660046327No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT13310.15369195559445645No Hit
CAATAATACACGGCCGTGTCGTCAGATCTGAGGTTCTTCACTTCCATATA13240.15288365830733308No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13210.15253724518428025No Hit
GTCCCCAAACTGATGATTTATGAGGTCAGTGATCGGCCCTCAGGGGTTTCTACTCGCTTCTCTGGCTCCAAGT12830.14814934562561055No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12660.14618633792831096No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT12630.1458399248052581No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12530.14468521439508186No Hit
GAGTCACCCTGACCACAGACACATCCACGAAAACAGCCTATATGGAAGTGAAGAACCTCAGATCTGACGACACGG12110.1398354306723417No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12100.13971995963132405No Hit
GGATAGGATGGATCAGCGCTTACAATGGAAACCCCAATTATGCAGAAAAATTCCAGGGCAGAGTCACCCTGACCA11840.13671771256486587No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11820.13648677048283062No Hit
ACCCAGGACGGTCAACTTGGTCCCTCCGCCGAAAAGCACGGTGGTTCTGC11760.1357939442367249No Hit
CCTGTAAGCCGTCTCGTCACGTCTTCACCACGTATGGCTTCAGTTGGGTG11510.1329071682112843No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11460.13232981300619617No Hit
GGATGGATCAGCGCTTACAATGGAAACCCCAATTATGCAGAAAAATTCCA11400.13163698676009042No Hit
GTATATGAGCTGCAATAATAATCAGCCTCGTCCTCAGGTTGGAGCCCAGA11380.1314060446780552No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11340.1309441605139847No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11240.12978945010380846No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11200.129327565939738No Hit
CTCCCAGGATCAACTGGTGCAGTCTGGAACTGAGGTGAAGAACCCTGGGG11160.12886568177566748No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11090.12805738448854412No Hit
GTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGTCCCCAAACTGATGA11070.12782644240650887No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11060.12771097136549125No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC10810.12482419534005068No Hit
CCTTGGGAGAATCCCCTAGATCACAGCTCCTCACCATGGACTGGACCTGGAACATCCTTTTCTTGGTGGCCGCAG10810.12482419534005068No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10500.12124459306850435No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG10390.1199744116173105No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10360.11962799849425762No Hit
ATAATACACGGCCGTGTCGTCAGATCTGAGGTTCTTCACTTCCATATAGGCTGTTTTCGTGGATGTGTCTGTGGT10290.11881970120713427No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC10280.11870423016611664No Hit
GTATTATTGTGTAAGTCTCCAGGCCCAAGCCCACTGGGACTGGGGCCAGG10250.11835781704306378No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT10240.11824234600204615No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10240.11824234600204615No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10180.11754951975594041No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10140.11708763559186992No Hit
CTTCTAAGTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGTCCCCAAA10130.1169721645508523No Hit
GTTTGGGGACTTTGCCTGGGTGCTGTTGGTACCAGGAGACATACTTAGAA10100.11662575142779943No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC10080.11639480934576417No Hit
TATACAGACATTACAGGCAGAACCACCGTGCTTTTCGGCGGAGGGACCAA10030.11581745414067607No Hit
GATTATTATTGCAGCTCATATACAGACATTACAGGCAGAACCACCGTGCT9920.11454727268948221No Hit
CATATACAGACATTACAGGCAGAACCACCGTGCTTTTCGGCGGAGGGACC9790.1130461491562531No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAAC9780.1129306781152355No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9710.11212238082811213No Hit
GTCCCAGTGGGCTTGGGCCTGGAGACTTACACAATAATACACGGCCGTGTCGTCAGATCTGAGGTTCTTCACTTC9580.11062125729488302No Hit
GGTACCAACAGCACCCAGGCAAAGTCCCCAAACTGATGATTTATGAGGTCAGTGATCGGCCCTCAGGGGTTTCTA9440.10900466272063629No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC9420.10877372063860104No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC9300.10738806814638957No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAACTTGGTCCCTCCGCCGAAAAGCACGGTGGTT9260.10692618398231908No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT9220.10646429981824859No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9200.10623335773621333No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC9160.10577147357214284No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT9160.10577147357214284No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT8980.10369299483382563No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8910.10288469754670226No Hit
GACCATCTCTGGGCTCCAACCTGAGGACGAGGCTGATTATTATTGCAGCTCATATACAGACATTACAGGCAGAAC8790.1014990450544908No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG8790.1014990450544908No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC8780.10138357401347316No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGAGTAGAAACCCCTGAGGGCCGAT8730.10080621880838504No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8730.10080621880838504No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT8730.10080621880838504No Hit
GGTTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC8670.1001133925622793No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGAA503.276086E-452.5336170
GGTATCA6000.049.695261
GCGAATC1050.045.8025933
CTTATAC1150.044.7033841
GACTAAG1100.043.62722426
AATGGTA3500.043.15260770
TCGTATG1050.042.751240
CGAATCT1050.042.5506834
CAACGGA409.3146897E-442.5020469
GGCGAAT1150.041.81733332
TAAGGCG1150.041.76174529
CACGAGA1150.041.71833821
TTATACA1250.041.1247372
CGTATGC1100.040.85074641
TCTCGTA1100.040.72503738
AGGCGAA1200.040.05639631
CGAGACT1200.039.98238423
TATACAC1200.039.9823843
CCGTTAA350.001393347439.16189616
ATGCCGT1250.038.82473844
Produced by FastQC (version 0.11.5)