FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866018
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC38160.44063749252325013No Hit
GCCTTGGGCTGACCCAGGACGGTCAACTTGGTCCCTCCGCCGAAAAGCAC37550.4335937590211751No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTC27110.31304199219877643No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24910.2876383631748994No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22470.2594634291665993No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAACTTGGTCCCTC21800.25172686941841854No Hit
CTCCTGGACAGTCGATCACCATCTCCTGTATTGGATCAAGTAGTGACGTT20730.23937146802953285No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC18590.21466066525176153No Hit
GTCTGGAACTGAGGTGAAGAACCCTGGGGCCTCAGTGAAGGTCTCCTGTA18410.21258218651344427No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17690.2042682715601754No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17520.20230526386287584No Hit
GTATTGGATCAAGTAGTGACGTTGGTACTTCTAAGTATGTCTCCTGGTAC17320.19999584304252338No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17230.19895660367336476No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16800.19399134890960695No Hit
CTCCTGTATTGGATCAAGTAGTGACGTTGGTACTTCTAAGTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGT16420.18960344935093726No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16280.18798685477669055No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG15630.18048123711054503No Hit
GTCGATCACCATCTCCTGTATTGGATCAAGTAGTGACGTTGGTACTTCTAAGTATGTCTCCTGGTACCAACAGCA15300.17667069275696348No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15300.17667069275696348No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15090.1742458008955934No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14860.17158996695218806No Hit
TCTCTGGGCTCCAACCTGAGGACGAGGCTGATTATTATTGCAGCTCATAT14310.1652390596962188No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14290.16500811761418355No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14220.16419982032706018No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13890.1603892759734786No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13800.15935003660431998No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG13680.15796438411210853No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13630.15738702890702042No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC13440.15519307912768557No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13220.15265271622529786No Hit
CCCCTGGACAAGGGCTTGAGTGGATAGGATGGATCAGCGCTTACAATGGA13210.15253724518428025No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13080.15103612165105113No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG12990.1499968822818925No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT12930.14930405603578678No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12880.14872670083069867No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12580.14526256960016998No Hit
GACCCAGGACGGTCAACTTGGTCCCTCCGCCGAAAAGCACGGTGGTTCTG12360.14272220669778227No Hit
GTCCCCAAACTGATGATTTATGAGGTCAGTGATCGGCCCTCAGGGGTTTCTACTCGCTTCTCTGGCTCCAAGT12030.1389116623442007No Hit
CAATAATACACGGCCGTGTCGTCAGATCTGAGGTTCTTCACTTCCATATA11980.13833430713911257No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT11620.13417734966247816No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11610.13406187862146052No Hit
GAGTCACCCTGACCACAGACACATCCACGAAAACAGCCTATATGGAAGTGAAGAACCTCAGATCTGACGACACGG11590.1338309365394253No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC11290.13036680530889658No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11270.13013586322686135No Hit
CCTGTAAGCCGTCTCGTCACGTCTTCACCACGTATGGCTTCAGTTGGGTG11160.12886568177566748No Hit
GGGTGACTCTGCCCTGGAATTTTTCTGCATAATTGGGGTTTCCATTGTAA10990.1269026740783679No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC10960.12655626095531503No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10850.12528607950412116No Hit
GGATGGATCAGCGCTTACAATGGAAACCCCAATTATGCAGAAAAATTCCA10840.12517060846310354No Hit
GTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGTCCCCAAACTGATGA10840.12517060846310354No Hit
ACCCAGGACGGTCAACTTGGTCCCTCCGCCGAAAAGCACGGTGGTTCTGC10810.12482419534005068No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10790.12459325325801542No Hit
GTATATGAGCTGCAATAATAATCAGCCTCGTCCTCAGGTTGGAGCCCAGA10670.12320760076580395No Hit
GTTTGGGGACTTTGCCTGGGTGCTGTTGGTACCAGGAGACATACTTAGAA10670.12320760076580395No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10640.12286118764275107No Hit
CTCCCAGGATCAACTGGTGCAGTCTGGAACTGAGGTGAAGAACCCTGGGG10580.12216836139664533No Hit
ATAATACACGGCCGTGTCGTCAGATCTGAGGTTCTTCACTTCCATATAGGCTGTTTTCGTGGATGTGTCTGTGGT10330.11928158537120476No Hit
GGATAGGATGGATCAGCGCTTACAATGGAAACCCCAATTATGCAGAAAAATTCCAGGGCAGAGTCACCCTGACCA10300.11893517224815188No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10220.1180114039200109No Hit
GTATTATTGTGTAAGTCTCCAGGCCCAAGCCCACTGGGACTGGGGCCAGG10190.11766499079695802No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC10150.11720310663288754No Hit
CCTTGGGAGAATCCCCTAGATCACAGCTCCTCACCATGGACTGGACCTGGAACATCCTTTTCTTGGTGGCCGCAG10020.11570198309965843No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9940.11477821477151745No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9870.11396991748439408No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9740.11246879395116498No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAAC9590.11073672833590063No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9520.10992843104877727No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9440.10900466272063629No Hit
GTCCCAGTGGGCTTGGGCCTGGAGACTTACACAATAATACACGGCCGTGTCGTCAGATCTGAGGTTCTTCACTTC9360.10808089439249531No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9340.10784995231046006No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT9310.10750353918740718No Hit
GATTATTATTGCAGCTCATATACAGACATTACAGGCAGAACCACCGTGCT9300.10738806814638957No Hit
CTTCTAAGTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGTCCCCAAA9300.10738806814638957No Hit
GGTTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC9280.1071571260643543No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAACTTGGTCCCTCCGCCGAAAAGCACGGTGGTT9280.1071571260643543No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT9210.10634882877723095No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG9190.10611788669519572No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9180.10600241565417809No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9170.10588694461316046No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT9120.10530958940807235No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9100.1050786473260371No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC9000.10392393691586088No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG8980.10369299483382563No Hit
CATATACAGACATTACAGGCAGAACCACCGTGCTTTTCGGCGGAGGGACC8960.10346205275179036No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGCTGACCAGGGTTCCCTGGCCCCAGTCCCAGTGGGCTTGGGC8950.10334658171077275No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8930.10311563962873752No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8760.10115263193143792No Hit
GGGTTCCCTGGCCCCAGTCCCAGTGGGCTTGGGCCTGGAGACTTACACAATAATACACGGCCGTGTCGTCAGAT8730.10080621880838504No Hit
GGTACCAACAGCACCCAGGCAAAGTCCCCAAACTGATGATTTATGAGGTCAGTGATCGGCCCTCAGGGGTTTCTA8720.10069074776736743No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC8680.10022886360329694No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG8680.10022886360329694No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT8680.10022886360329694No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)