FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1289713
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT38640.2996015392571836No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34710.2691296435718644No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34140.2647100556480395No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27200.21089963425971514No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA25860.2005097258072145No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGACTGCTGATGGTGAGAGTGAACT24280.18825893822889278No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG23720.18391688693531041No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23450.1818233979187618No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22650.17562046749935836No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22040.1708907330545633No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21800.16902985392874229No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA20910.162129093837156No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20410.1582522623250289No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20350.15778704254357365No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20040.15538340700605482No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGTCTGCAGTCTGAAGATT20030.15530587037581228No Hit
GAGTTCACTCTCACCATCAGCAGTCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAACTGG19450.15080874582174483No Hit
GTAATATACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGGTACA19420.1505761359310172No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG18870.14631162126767738No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18440.14297754616724806No Hit
CATCTATGGTGCATCTAACAGGGTCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA17610.13654200585711704No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17560.13615432270590436No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17320.13429344358008333No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17230.13359561390790042No Hit
CTCCTGCAGGGCCAGTCAGACTATTAGCAGCTTCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT16870.13080429521916892No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC16680.12933109924456063No Hit
GTCTCCAGCCACCCTGTCTCTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTATTAGCAG16410.12723761022801197No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC15610.12103467980860859No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGACCCTGTT15490.12010424024569807No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA15380.1192513373130301No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15120.117235384926724No Hit
GGCCAAAAGTGTACGGAGGCCAGTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGACTG15040.11661509188478367No Hit
GTTTATTACTGTCAGCAGTATAATAACTGGCCTCCGTACACTTTTGGCCA15030.11653755525454113No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG14890.11545204243114554No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGAATCACTTTCAGTGACGCCTGG14690.11390130982629469No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT14630.11343609004483943No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14630.11343609004483943No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC14610.11328101678435434No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC14120.10948172190246978No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC13770.10676793984398078No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13740.10653532995325316No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTCTGTCTCCAGG13090.10149544898748791No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13070.10134037572700283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG4000.052.5649740
CGTATGC4250.050.3758441
ATCTCGT2700.048.12994437
ATGCCGT4700.047.3247744
AGCGATC2900.046.8685133
GAGCGAT4800.045.9602332
TGCCGTC4700.045.21671345
GTCGTAT1750.045.18541739
CCGTCTT4900.043.5632147
GCCGTCT4850.042.466746
TGAGCGA5350.041.8240131
CACGAGA5500.041.6820621
TGTCGTA1900.041.50322738
ACGAGAC5650.041.1753522
GGTATCA16150.039.868521
CGAGACA5900.039.43675223
CTTATAC6200.039.1480451
TATACAC6300.039.055683
CTGAGCG5900.039.0165830
CGAGCCC5900.038.8365815
Produced by FastQC (version 0.11.5)