FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1040320
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATACCTGGCCTCACCCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT145211.3958205167640725No Hit
GTATTATACTGTTGACAGTAATAAATTGCAAAATCTTCAGACTGCAGGCTGCTGATGCTGAGAGTGAACTCTGTC117701.1313826514918488No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT61150.5877999077207013No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC51400.49407874500153803No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA48910.47014380190710553No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39700.381613349738542No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34520.331820978160566No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32380.3112503844970778No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31770.3053868040602891No Hit
GTCTGGGACAGAGTTCACTCTCAGCATCAGCAGCCTGCAGTCTGAAGATT31260.300484466318056No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31240.3002922177791449No Hit
GTCCCAGACCCACTGCCACTAAACCTGTCTGGGATACCAGTGGCCCTGAT30530.2934673946478007No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29830.286738695785912No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29030.27904875422946784No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAACAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT28690.27578052906797906No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28350.2725123039064903No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28200.271070439864657No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27000.25953552752999076No Hit
GTCTCCAGACACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAACAG26610.25578668102122426No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26530.25501768686557985No Hit
GAGTTCACTCTCAGCATCAGCAGCCTGCAGTCTGAAGATTTTGCAATTTATTACTGTCAACAGTATAATACCTGG26510.2548254383266687No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC25100.2412719163334359No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24160.232236235004614No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23950.23021762534604734No Hit
CCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAACAGCAACTTAGC22720.21839434020301446No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGACACCCTGTCTGTGTCTCCAGG22580.21704860043063676No Hit
TCTCTGGTGCATCCATCAGGGCCACTGGTATCCCAGACAGGTTTAGTGGCAGTGGGTCTGGGACAGAGTTCACT22400.2153183635804368No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21800.20955090741310364No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTAAACCTGTCTGGGATACCA20870.2006113503537373No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20860.20051522608428174No Hit
CTGGTATCCCAGACAGGTTTAGTGGCAGTGGGTCTGGGACAGAGTTCACT20700.19897723777299292No Hit
CTCCAGACACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC20350.19561288834204862No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT20210.19426714856967087No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19750.18984543217471547No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19280.1853275915103045No Hit
GGTTTAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCAGCATCAGCAGC19260.18513534297139342No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGGG18900.18167486927099355No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18440.17725315287603813No Hit
GAGTGTTAACAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTCTGGTGCATC18200.17494617040910487No Hit
CCTCATCTCTGGTGCATCCATCAGGGCCACTGGTATCCCAGACAGGTTTAGTGGCAGTGGGTCTGGGACAGAGTT18020.17321593355890494No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17840.171485696708705No Hit
CCAGACACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAACAGCAA17690.17004383266687173No Hit
GTGTTAACAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCC17620.16937096278068287No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17430.1675446016610274No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTCTGGTGCATCCATCA16360.1572593048292833No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAACAGCAACTTAGCCTGGTA16330.15697093202091664No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16180.15552906797908336No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16130.1550484466318056No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTC16030.15408720393725006No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15360.14764687788372807No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15350.14755075361427253No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15290.1469740079975392No Hit
CACTAAACCTGTCTGGGATACCAGTGGCCCTGATGGATGCACCAGAGATG15020.14437865272223932No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14760.14187942171639495No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14710.14139880036911717No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14530.13966856351891724No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC14470.13909181790218395No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14460.13899569363272837No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14440.13880344509381728No Hit
GGATACCAGTGGCCCTGATGGATGCACCAGAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG14410.13851507228545062No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14390.13832282374653954No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14370.13813057520762842No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14280.13726545678252844No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14190.13640033835742849No Hit
CATCAGGGCCACTGGTATCCCAGACAGGTTTAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCAGCATCAGCAG14140.13591971701015074No Hit
CCCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCAT14140.13591971701015074No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14050.13505459858505076No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14040.13495847431559518No Hit
GTCAGAGTGTTAACAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTCTGGTG14020.1347662257766841No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTC14020.1347662257766841No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC13950.13409335589049523No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAACAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG13780.13245924330975084No Hit
CCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTCCAGACACCCTGTCTGTGTCTCCAGGGGAAAGAGCCAC13320.12803752691479545No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13280.12765302983697324No Hit
CACCAGAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTGCTGTTAACACTCTGA13280.12765302983697324No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13260.12746078129806213No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT13160.12649953860350663No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13070.12563442017840665No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTCCAGACACC13030.12524992310058444No Hit
GTAATACACAGCCGTGTCTTCAGAGGTCAGGCTGGTCAGGTCCATGTAGA13020.12515379883112887No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12970.12467317748385114No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12900.12400030759766226No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT12870.12371193478929561No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12590.12102045524454014No Hit
GGTTTCTGCTGGTACCAGGCTAAGTTGCTGTTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCC12490.12005921254998463No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12400.11919409412488464No Hit
GAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC12190.11717548446631806No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12110.11640649031067363No Hit
GAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTGCTGTTAACACTCTGACTGGCC12100.11631036604121808No Hit
CTCCCAGGCTCCTCATCTCTGGTGCATCCATCAGGGCCACTGGTATCCCA12090.11621424177176254No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12060.11592586896339586No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12040.11573362042448478No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11960.11496462626884037No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11920.11458012919101815No Hit
ATCCCAGACAGGTTTAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCAG11680.1122731467240849No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT11670.11217702245462935No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC11500.11054290987388496No Hit
GGTTTAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCAGCATCAGCAGCCTGCAGTCTGAAGATTTTGCAATTT11390.10948554290987388No Hit
CCACTGGAGAAATAGTGATGACGCAGTCTCCAGACACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTC11390.10948554290987388No Hit
GTAATAAATTGCAAAATCTTCAGACTGCAGGCTGCTGATGCTGAGAGTGA11170.10737080898185175No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10870.10448708089818518No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10820.10400645955090741No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10800.1038142110119963No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC10700.10285296831744078No Hit
ATTTATTACTGTCAACAGTATAATACCTGGCCTCACCCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA10700.10285296831744078No Hit
TACCTGGCCTCACCCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT10580.10169947708397417No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTGCTGTTA10520.10112273146724085No Hit
ACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAACAGCAACTTAGCCTGGTA10490.10083435865887419No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT150.002377146468.8408339
AAGTCGC200.006350520553.71462663
TTTCGCG200.006830464652.7342754
GGTATCA9450.041.4764181
CTAACGC504.1830826E-640.891493
CTTATAC3100.037.381131
CGCTCAT3200.037.3319128
CCTAACG558.047915E-637.174082
CGAGACG3100.036.29564723
AGGGACC32750.036.2253731
TTCGGCG32850.036.1198723
CGTATGC2200.036.08133741
GAGGGAC32700.036.0455730
TAATACC33250.035.9722144
GTATCAA24600.035.883921
TTTCGGC33200.035.73393222
TCGGCGG33050.035.69839524
CGGAGGG33200.035.57133528
CGGCGGA33300.035.43891525
AATACCT33900.035.3835
Produced by FastQC (version 0.11.5)