FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004688862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1040320
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATACCTGGCCTCACCCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT145381.397454629344817No Hit
GTATTATACTGTTGACAGTAATAAATTGCAAAATCTTCAGACTGCAGGCTGCTGATGCTGAGAGTGAACTCTGTC117661.1309981544140264No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60100.5777068594278684No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA46850.45034220239926176No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43870.4216971701015072No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39670.3813249769301753No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32760.31490310673638877No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32050.30807828360504463No Hit
GTCTGGGACAGAGTTCACTCTCAGCATCAGCAGCCTGCAGTCTGAAGATT31200.29990772070132266No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31150.2994270993540449No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30980.2977929867733005No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29360.2822208551215011No Hit
GTCCCAGACCCACTGCCACTAAACCTGTCTGGGATACCAGTGGCCCTGAT29050.279241002768379No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAACAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT28220.2712626884035681No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26650.25617117809904644No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26470.2544409412488465No Hit
GAGTTCACTCTCAGCATCAGCAGCCTGCAGTCTGAAGATTTTGCAATTTATTACTGTCAACAGTATAATACCTGG26180.2516533374346355No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25680.24684712396185787No Hit
GTCTCCAGACACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAACAG25480.24492463857274685No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24790.23829206398031372No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24760.2380036911719471No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24620.23665795139956938No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC24170.2323323592740695No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23410.22502691479544756No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23390.22483466625653645No Hit
CCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAACAGCAACTTAGC23240.22339280221470317No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTAAACCTGTCTGGGATACCA22370.2150299907720701No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGACACCCTGTCTGTGTCTCCAGG22200.21339587819132572No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21620.20782067056290374No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21590.20753229775453708No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21500.20666717932943712No Hit
TCTCTGGTGCATCCATCAGGGCCACTGGTATCCCAGACAGGTTTAGTGGCAGTGGGTCTGGGACAGAGTTCACT21100.202822208551215No Hit
GGTTTAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCAGCATCAGCAGCCTGCAGTCTGAAGATTTTGCAATTT21020.2020532143955706No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT20490.19695862811442635No Hit
CTGGTATCCCAGACAGGTTTAGTGGCAGTGGGTCTGGGACAGAGTTCACT20320.19532451553368196No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGGG19330.18580821285758228No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19200.1845585973546601No Hit
CTCCAGACACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC18630.17907951399569363No Hit
GTGTTAACAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCC18330.1761957859120271No Hit
CCTCATCTCTGGTGCATCCATCAGGGCCACTGGTATCCCAGACAGGTTTAGTGGCAGTGGGTCTGGGACAGAGTT18160.1745616733312827No Hit
GAGTGTTAACAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTCTGGTGCATC17330.16658335896647186No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16940.1628345124577053No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTC16920.16264226391879422No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16750.16100815133804983No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAACAGCAACTTAGCCTGGTA16550.15908566594893878No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16180.15552906797908336No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16120.15495232236235004No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTCTGGTGCATCCATCA16080.15456782528452784No Hit
CCAGACACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAACAGCAA15960.1534143340510612No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15530.14928099046447246No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15430.14831974776991694No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15370.14774300215318362No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15090.14505152260842819No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15030.14447477699169486No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14870.14293678868040602No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14670.141014303291295No Hit
CCCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCAT14560.13995693632728393No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14480.13918794217163952No Hit
CACTAAACCTGTCTGGGATACCAGTGGCCCTGATGGATGCACCAGAGATG14200.13649646262688403No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13940.1339972316210397No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTC13940.1339972316210397No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13810.1327476161181175No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13770.1323631190402953No Hit
CATCAGGGCCACTGGTATCCCAGACAGGTTTAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCAGCATCAGCAG13750.13217087050138418No Hit
GGATACCAGTGGCCCTGATGGATGCACCAGAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG13410.12890264533989543No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTCCAGACACC13330.128133651184251No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13230.1271724084896955No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13220.12707628422023992No Hit
GGTTTCTGCTGGTACCAGGCTAAGTTGCTGTTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCC13050.12544217163949553No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT12930.12428868040602892No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12920.12419255613657337No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12880.12380805905875115No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC12840.12342356198092894No Hit
GTCAGAGTGTTAACAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTCTGGTG12710.12217394647800676No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12660.121693325130729No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12650.12159720086127344No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAACAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG12610.12121270378345125No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12330.1185212242386958No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12060.11592586896339586No Hit
GAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC12020.11554137188557367No Hit
CCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTCCAGACACCCTGTCTGTGTCTCCAGGGGAAAGAGCCAC11910.1144840049215626No Hit
GGTTTAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCAGCATCAGCAGC11830.1137150107659182No Hit
GTAATACACAGCCGTGTCTTCAGAGGTCAGGCTGGTCAGGTCCATGTAGA11790.11333051368809598No Hit
GTAATAAATTGCAAAATCTTCAGACTGCAGGCTGCTGATGCTGAGAGTGA11660.1120808981851738No Hit
CACCAGAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTGCTGTTAACACTCTGA11650.11198477391571825No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11570.11121577976007382No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTGCTGTTA11490.11044678560442941No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11450.1100622885266072No Hit
CCACTGGAGAAATAGTGATGACGCAGTCTCCAGACACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTC11410.10967779144878499No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11360.10919717010150723No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT11270.10833205167640725No Hit
CTCCCAGGCTCCTCATCTCTGGTGCATCCATCAGGGCCACTGGTATCCCA11220.10785143032912949No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11190.10756305752076284No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11160.10727468471239618No Hit
GGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAACAGCAACTTAGCCTGGTACCAGCAGAAAC11130.10698631190402953No Hit
ATTTATTACTGTCAACAGTATAATACCTGGCCTCACCCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA11090.10660181482620731No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11080.10650569055675177No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC11060.10631344201784067No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11010.1058328206705629No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10990.1056405721316518No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10970.10544832359274069No Hit
GAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTGCTGTTAACACTCTGACTGGCC10960.10535219932328514No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10910.10487157797600738No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT10860.10439095662872962No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10680.10266071977852967No Hit
GTCTGAAGATTTTGCAATTTATTACTGTCAACAGTATAATACCTGGCCTC10590.10179560135342972No Hit
ATCCCAGACAGGTTTAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCAG10520.10112273146724085No Hit
ATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACG10520.10112273146724085No Hit
GGGTGAGGCCAGGTATTATACTGTTGACAGTAATAAATTGCAAAATCTTC10470.10064211011996309No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)