FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1522931
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60090.394568105843272No Hit
GTTAGGACCCAGACGGACCCATGGAAGCCCCAGCGCAGCTTCTCCTCCTCCTGCTACTCTGGCTCCCAGAGACCA54190.3558270203968532No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC53140.3489324204445244No Hit
GTATGAACGTTGGCCTCCACTCTCTTTCGGCAGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT51610.33888600337113106No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41250.2708592838414872No Hit
GAGTTCACCCTCACCATCAGCAGCCTGCAATCAGAGGACGTGGGGGTTTATTTCTGTCAGCAGTATGAACGTTGG41080.26974301527777683No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36780.24150798690157335No Hit
CGTTCATACTGCTGACAGAAATAAACCCCCACGTCCTCTGATTGCAGGCTGCTGATGGTGAGGGTGAACTCTGTC35610.23382543266897843No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34060.2236476898822074No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33910.22266274703187472No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG32860.21576814707954597No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32180.21130307282470448No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32000.21012114140430524No Hit
CTCCTGCAGGGCCAGTGGAAGTATTGGTACCAAGTTAGCGTGGTATCACCAGAGACCTGGCCAGGCTCCCAGGCT31800.20880788427052832No Hit
CCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATGTATGATGCATCTATCA31130.2044084728723757No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30490.2002060500442896No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30460.20000906147422307No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30270.198761467197135No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29490.19363976437540506No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29140.1913415643912955No Hit
GTCTGTGTCTCCAGGGGAGACAGTCACCCTCTCCTGCAGGGCCAGTGGAA29040.19068493582440701No Hit
GTGTCTGGTGGCTCCATCATTAATAGGAATCATTATTGGAATTGGGTCCG28160.18490660443578863No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC26750.1756481416426614No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26180.17190535881139724No Hit
CCTCATGTATGATGCATCTATCAGGGCGACACTTATCCCCGCCAGATTTGCTGGCACTGCGTCTGGGACAGAGTT25750.1690818559737769No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23260.15273180465825437No Hit
GTCCCAGATGGTCCTGCAGGAGTCGGGCCCAGCGCTAGTGAGGCCTTCAG23200.1523378275181213No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23100.15168119895123286No Hit
GTCTGGGACAGAGTTCACCCTCACCATCAGCAGCCTGCAATCAGAGGACG22600.14839805611679058No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22480.14761010183652443No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22270.14623118184605868No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCTGCCGAAAGAGAGTGGAGGCCAACGTTCATACTG22250.146099856132681No Hit
GCCGAAAGAGAGTGGAGGCCAACGTTCATACTGCTGACAGAAATAAACCCCCACGTCCTCTGATTGCAGGCTG22160.14550889042248139No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGCTTCTTCCTCCTGCTGGTGGCGGCTCCCAGAGGGGGC22090.14504925042565947No Hit
GGGTGAACTCTGTCCCAGACGCAGTGCCAGCAAATCTGGCGGGGATAAGT22040.14472093614221526No Hit
GACCCATGGAAGCCCCAGCGCAGCTTCTCCTCCTCCTGCTACTCTGGCTCCCAGAGACCACTGGACAACTGGTG21840.14340767900843834No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21830.1433420161517495No Hit
GTATTGGTACCAAGTTAGCGTGGTATCACCAGAGACCTGGCCAGGCTCCC21550.1415034561644618No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21270.13966489617717415No Hit
GTCCCAGACGCAGTGCCAGCAAATCTGGCGGGGATAAGTGTCGCCCTGAT21220.1393365818937299No Hit
GTCTCCCGCCACCCTGTCTGTGTCTCCAGGGGAGACAGTCACCCTCTCCTGCAGGGCCAGTGGAAGTATTGGTAC20760.13631609048604304No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20650.13559379906246574No Hit
GCTCAGTTAGGACCCAGACGGACCCATGGAAGCCCCAGCGCAGCTTCTCC20310.133361261935045No Hit
GGTCTCTGGTGATACCACGCTAACTTGGTACCAATACTTCCACTGGCCCTGCAGGAGAGGGTGACTGTCTCCC20310.133361261935045No Hit
CTCTTTCGGCAGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCAT19940.13093173623755772No Hit
GAAATACACGGCCGAGTCCGCGGCAGTCACAGAGCTCAGTTGCATGGAAAACTGGTTCTTGGACCTGTCTACTGA19450.1277142562598043No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19450.1277142562598043No Hit
CCACTGGACAACTGGTGCTGACGCAGTCTCCCGCCACCCTGTCTGTGTCTCCAGGGGAGACAGTCACCCTCTC19390.1273202791196712No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19270.12653232483940507No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18960.12449677628205086No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18780.12331484486165165No Hit
CAGTCACCCTCTCCTGCAGGGCCAGTGGAAGTATTGGTACCAAGTTAGCGTGGTATCACCAGAGACCTGGCCAGG18310.12022869059727592No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18000.11819314203992169No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17990.11812747918323287No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17930.11773350204309979No Hit
CACTTATCCCCGCCAGATTTGCTGGCACTGCGTCTGGGACAGAGTTCACC17720.11635458205263402No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17650.11589494205581212No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17510.11497566206216828No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17050.11195517065448138No Hit
CTGCTACTCTGGCTCCCAGAGACCACTGGACAACTGGTGCTGACGCAGTC16820.11044492495063796No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16760.11005094781050488No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16760.11005094781050488No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16630.10919733067354988No Hit
GTGGAAGTATTGGTACCAAGTTAGCGTGGTATCACCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATGTATGATG16560.10873769067672796No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16540.10860636496335027No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16520.10847503924997258No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16510.10840937639328375No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16330.10722744497288453No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16230.10657081640599608No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16190.1063081649792407No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16060.1054545478422857No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT16060.1054545478422857No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15870.10420695356519764No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15830.10394430213844226No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15730.1032876735715538No Hit
GTCCTGCAGGAGTCGGGCCCAGCGCTAGTGAGGCCTTCAGAGACCCTGTCCCTCACGTGCACTGTGTCTGGTGG15650.10276237071804303No Hit
GGGCGACACTTATCCCCGCCAGATTTGCTGGCACTGCGTCTGGGACAGAG15630.10263104500466536No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15420.10125212501419958No Hit
GTATCACCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATGTATGATGCATCTATCAGGGCGACACTTATCCCCGC15400.10112079930082189No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15310.10052983359062229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA750.059.4967138
CGCGTAT800.055.88557439
ATCGCGT1000.051.44322237
AACGTCA400.00835852450.10778870
CGTATGC2400.048.88141641
TGCATCG1550.048.54245834
CTACGCT4700.046.39914328
TCGTATG2350.045.45604740
ACTACGC4800.045.4145727
TACGCTG4900.044.53313429
ATCTCGT2550.044.38238537
ACGAGAC5150.043.6021522
CGAGACT5100.043.36678323
GCCGTCT2500.043.1376546
TATGGCG651.1044904E-842.6090443
TATACAC5700.042.3710443
ACGCTGC5250.042.23733530
CACGAGA5350.041.97078721
TTATACA5600.041.9128042
GCGTATG1000.041.3503440
Produced by FastQC (version 0.11.5)