FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1522931
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58810.38616326018709973No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58170.3819608373590136No Hit
GTATGAACGTTGGCCTCCACTCTCTTTCGGCAGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT52160.3424974604890176No Hit
GTTAGGACCCAGACGGACCCATGGAAGCCCCAGCGCAGCTTCTCCTCCTCCTGCTACTCTGGCTCCCAGAGACCA49420.3245058377562739No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46130.3029027579056438No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42600.27972376949448136No Hit
GAGTTCACCCTCACCATCAGCAGCCTGCAATCAGAGGACGTGGGGGTTTATTTCTGTCAGCAGTATGAACGTTGG40230.264161672459225No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35700.23441639837917805No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35550.23343145552884537No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33760.22167780418154204No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32360.2124850042451037No Hit
CGTTCATACTGCTGACAGAAATAAACCCCCACGTCCTCTGATTGCAGGCTGCTGATGGTGAGGGTGAACTCTGTC31360.20591871857621913No Hit
CTCCTGCAGGGCCAGTGGAAGTATTGGTACCAAGTTAGCGTGGTATCACCAGAGACCTGGCCAGGCTCCCAGGCT30780.2021102728882661No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29720.19515001007924848No Hit
GTGTCTGGTGGCTCCATCATTAATAGGAATCATTATTGGAATTGGGTCCG28810.18917469012056357No Hit
GGGTGAACTCTGTCCCAGACGCAGTGCCAGCAAATCTGGCGGGGATAAGT28780.18897770155049703No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28780.18897770155049703No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28360.18621986156956552No Hit
GTCTGTGTCTCCAGGGGAGACAGTCACCCTCTCCTGCAGGGCCAGTGGAA28060.18424997586890016No Hit
CCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATGTATGATGCATCTATCA27520.18070418160770252No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27290.17919393590385907No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG27010.1773553759165714No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26210.17210234738146377No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25980.17059210167762032No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25850.16973848454066534No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25210.16553606171257923No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC25110.16487943314569078No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGCTTCTTCCTCCTGCTGGTGGCGGCTCCCAGAGGGGGC24920.1636318388686027No Hit
CCTCATGTATGATGCATCTATCAGGGCGACACTTATCCCCGCCAGATTTGCTGGCACTGCGTCTGGGACAGAGTT23780.15614627320607435No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22690.1489890218269902No Hit
GTCCCAGATGGTCCTGCAGGAGTCGGGCCCAGCGCTAGTGAGGCCTTCAG22300.14642817041612524No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21980.14432695900208217No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCTGCCGAAAGAGAGTGGAGGCCAACGTTCATACTG21950.14412997043201564No Hit
GTCTGGGACAGAGTTCACCCTCACCATCAGCAGCCTGCAATCAGAGGACG21530.14137213045108413No Hit
GTCCCAGACGCAGTGCCAGCAAATCTGGCGGGGATAAGTGTCGCCCTGAT21420.14064983902750683No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21080.13841730190008608No Hit
GCCGAAAGAGAGTGGAGGCCAACGTTCATACTGCTGACAGAAATAAACCCCCACGTCCTCTGATTGCAGGCTG20980.13776067333319764No Hit
GACCCATGGAAGCCCCAGCGCAGCTTCTCCTCCTCCTGCTACTCTGGCTCCCAGAGACCACTGGACAACTGGTG20860.13697271905293149No Hit
GGTCTCTGGTGATACCACGCTAACTTGGTACCAATACTTCCACTGGCCCTGCAGGAGAGGGTGACTGTCTCCC20810.13664440476948728No Hit
CCACTGGACAACTGGTGCTGACGCAGTCTCCCGCCACCCTGTCTGTGTCTCCAGGGGAGACAGTCACCCTCTC20160.13237631908471229No Hit
GCTCAGTTAGGACCCAGACGGACCCATGGAAGCCCCAGCGCAGCTTCTCC19860.13040643338404695No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19830.13020944481398042No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19670.12915883910695888No Hit
GTATTGGTACCAAGTTAGCGTGGTATCACCAGAGACCTGGCCAGGCTCCC19630.1288961876802035No Hit
CTCTTTCGGCAGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCAT19610.1287648619668258No Hit
GTCTCCCGCCACCCTGTCTGTGTCTCCAGGGGAGACAGTCACCCTCTCCTGCAGGGCCAGTGGAAGTATTGGTAC19540.1283052219700039No Hit
GGGCGACACTTATCCCCGCCAGATTTGCTGGCACTGCGTCTGGGACAGAG19540.1283052219700039No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19350.12705762769291581No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18860.12384014771516241No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18700.12278954200814088No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18680.12265821629476319No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18500.12147628487436397No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18500.12147628487436397No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18310.12022869059727592No Hit
CACTTATCCCCGCCAGATTTGCTGGCACTGCGTCTGGGACAGAGTTCACC18080.11871844489343246No Hit
GAAATACACGGCCGAGTCCGCGGCAGTCACAGAGCTCAGTTGCATGGAAAACTGGTTCTTGGACCTGTCTACTGA17960.11793049061316631No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17520.11504132491885713No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17360.11399071921183561No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA17270.11339975350163599No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17240.11320276493156946No Hit
GTGGAAGTATTGGTACCAAGTTAGCGTGGTATCACCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATGTATGATG17210.11300577636150291No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17090.11221782208123676No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16830.11051058780732678No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16800.11031359923726027No Hit
GTCCTGCAGGAGTCGGGCCCAGCGCTAGTGAGGCCTTCAGAGACCCTGTCCCTCACGTGCACTGTGTCTGGTGG16710.10972263352706065No Hit
CAGTCACCCTCTCCTGCAGGGCCAGTGGAAGTATTGGTACCAAGTTAGCGTGGTATCACCAGAGACCTGGCCAGG16650.10932865638692757No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16620.10913166781686104No Hit
CTGCTACTCTGGCTCCCAGAGACCACTGGACAACTGGTGCTGACGCAGTC16600.10900034210348335No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16320.10716178211619568No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15800.10374731356837572No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15750.10341899928493149No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15660.10282803357473187No Hit
GTGTCGCCCTGATAGATGCATCATACATGAGGAGCCTGGGAGCCTGGCCA15390.10105513644413305No Hit
GCTTCTCCTCCTCCTGCTACTCTGGCTCCCAGAGACCACTGGACAACTGGTGCTGACGCAGTCTCCCGCCACC15370.10092381073075536No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15280.10033284502055577No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT554.1381794E-450.10919670
GTATCAA31200.038.8888971
GTACGGT350.001450969538.842211
TAAGCCG400.00269377534.2424335
TATCAAC35750.033.8209882
ATCAACG36900.032.846043
TCAACGC37200.032.6724174
CAACGCA37800.032.153815
AACGCAG38000.031.9845756
ACGCAGA40050.030.3474127
CGCAGAG40400.030.3366078
AGAGTAC41400.029.68681711
GCAGAGT42400.028.9065849
GTACGTA650.00836101127.16320869
GAGTTTA4800.026.9063221
TGTTAGA16700.025.99227170
AGTTTAT5150.025.0604382
CAGAGTA49450.024.8540810
AGTACGG37550.024.4123313
ATTCACT2650.024.3511832
Produced by FastQC (version 0.11.5)