FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences567427
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC34490.6078314919804662No Hit
GCCTTGGGCTGACCCAGGACGGTCAGTCTGGTCCCCCCGCCGAATGGGATACCGTACGAGCTTGCAAAGGAGCAG29520.5202431325967921No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT25270.44534363010572287No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23530.4146788926152615No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTGTCTGCCTTCCAG21940.38665766697742615No Hit
GTCCTGGGCCCAGGCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG20040.3531731835108305No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19120.3369596441480578No Hit
CTCTAAGAGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCT18570.3272667673550959No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCTCTGGAAGCAGCGATGACATT18510.32620936261404554No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGTCTGGTCCCCC18340.32321338251440274No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17650.3110532279923232No Hit
GATTATTACTGCTGCTCCTTTGCAAGCTCGTACGGTATCCCATTCGGCGGGGGGACCAGACTGACCGTCCTGGGT17590.30999582325127284No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16310.28743785544219785No Hit
GTAATAATCAGCTTCATCGTCAGGTCGGAGCCCAGAGACGGTCAGGGAGG15660.2759826374141519No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15380.2710480819559168No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14670.2585354591868205No Hit
CCACCACACCCTCCAAACAAAACAACAACAAGTACGCGGCCAGCAGCTAT14660.2583592250633121No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG13760.24249815394755625No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGTTTTGTTTGGAGGGTGTGGTGGTCTCCA13630.24020711034194708No Hit
GGGCACAGGGTCCTGGGCCCAGGCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC13420.23650619374827067No Hit
GTCTGGTCCCCCCGCCGAATGGGATACCGTACGAGCTTGCAAAGGAGCAG13350.2352725548837119No Hit
GTCAGTCACCATCTCCTGCTCTGGAAGCAGCGATGACATTGGTGCTTCTA13240.23333397952511956No Hit
TCTCTGGGCTCCGACCTGACGATGAAGCTGATTATTACTGCTGCTCCTTT13060.23016176530196836No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12870.2268133169553088No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGTTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12710.2239935709791744No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12530.22082135675602324No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12520.22064512263251487No Hit
CTCTTAGAGACCTCAAAAATGATGAGTTTGGGGGCTTTGCCTGGATATTG12220.2153580989272629No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG12190.2148293965567377No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12140.21394822593919569No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11970.21095224583955294No Hit
ATGGGATACCGTACGAGCTTGCAAAGGAGCAGCAGTAATAATCAGCTTCA11040.19456247235327187No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGGCTGCCCTGACTCAGC10590.18663193679539394No Hit
GTATCCCATTCGGCGGGGGGACCAGACTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC10450.18416465906627635No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10180.17940633773154963No Hit
GGACCAGACTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG9800.17270944103823047No Hit
CCGTACGAGCTTGCAAAGGAGCAGCAGTAATAATCAGCTTCATCGTCAGG9770.17218073866770528No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC9720.17129956805016328No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT9720.17129956805016328No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9710.17112333392665488No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9580.1688322903210457No Hit
GGGCTGCTGTCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9550.1683035879505205No Hit
ACAATATCCAGGCAAAGCCCCCAAACTCATCATTTTTGAGGTCTCTAAGAGGCCCTCAGGGGTCCCTGATCGCTT9550.1683035879505205No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGGCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT9330.16442643723333575No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGGCTGCCCTGACT9230.16266409599825174No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9220.16248786187474337No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC9100.16037305239264257No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCTCTGGAAGCAGCGATGACATTGGTG9040.15931564765159217No Hit
GTTTGGGGGCTTTGCCTGGATATTGTTGGTACCAGGAGACAAAGTTAGAA8910.157024604045983No Hit
CTCCTGCTCTGGAAGCAGCGATGACATTGGTGCTTCTAACTTTGTCTCCTGGTACCAACAATATCCAGGCAAAGC8810.15526226281089903No Hit
CCCCTGAGGGCCTCTTAGAGACCTCAAAAATGATGAGTTTGGGGGCTTTGCCTGGATATTGTTGGTACCAGGAGA8770.15455732631686545No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8700.15332368745230665No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG8670.15279498508178144No Hit
CTCGTACGGTATCCCATTCGGCGGGGGGACCAGACTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCA8570.15103264384669746No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8560.15085640972318906No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC8550.15068017559968067No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGTCTGGTCCCCCCGCCGAATGGGATAC8500.14979900498213866No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8400.14803666374705468No Hit
GACCCAGGACGGTCAGTCTGGTCCCCCCGCCGAATGGGATACCGTACGAGCTTGCAAAGGAGCAGCAGTAATAAT8330.1468030248824959No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC8160.14380704478285314No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7590.1337616997428744No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7570.13340923149585762No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT7480.13182312438428204No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC7440.13111818789024846No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG7380.13006078314919803No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7360.12970831490218127No Hit
ACCCAGGACGGTCAGTCTGGTCCCCCCGCCGAATGGGATACCGTACGAGC7350.12953208077867284No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCGAC7150.12600739830850488No Hit
GCTCTGGAAGCAGCGATGACATTGGTGCTTCTAACTTTGTCTCCTGGTAC7070.1245975253204377No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGGCTGCCCTGACTCAGCCT6910.12177777934430332No Hit
CTCCCTGACCGTCTCTGGGCTCCGACCTGACGATGAAGCTGATTATTACT6850.12072037460325294No Hit
CCCTGACCGTCTCTGGGCTCCGACCTGACGATGAAGCTGATTATTACTGC6730.11860556512115214No Hit
CAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAACAACAACAAGTACGCGGCCAGCAG6710.11825309687413534No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC6590.11613828739203456No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGACAGCAGCC6560.11560958502150939No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG6520.11490464852747578No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTGTCTGCCTTCCAGGCCACTGTCA6510.11472841440396737No Hit
GGCCCAGGCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGAC6470.1140234779099338No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAACAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC6470.1140234779099338No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC6450.11367100966291699No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT6430.11331854141590018No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC6410.11296607316888341No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG6250.11014632719274903No Hit
GGCCTGGAAGGCAGACAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA6080.10715034709310625No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC6070.10697411296959784No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTGTCTGCCTTCCAGGCCACTGTCACGGCTC6060.10679787884608945No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC6040.10644541059907267No Hit
CTTAGAGACCTCAAAAATGATGAGTTTGGGGGCTTTGCCTGGATATTGTT6000.10574047410503906No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG5970.10521177173451386No Hit
GTCTCTAAGAGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGT5930.10450683524048027No Hit
GTCACCATCTCCTGCTCTGGAAGCAGCGATGACATTGGTGCTTCTAACTT5930.10450683524048027No Hit
GGATATTGTTGGTACCAGGAGACAAAGTTAGAAGCACCAATGTCATCGCTGCTTCCAGAGCAGGAGATGGTGA5900.10397813286995507No Hit
GCCCCCAAACTCATCATTTTTGAGGTCTCTAAGAGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGT5780.1018633233878543No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC5770.1016870892643459No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3700.075.2342970
TCGTATG4150.050.70592540
TCTCGTA4150.049.75033638
CGTATGC4250.049.61470441
CTTATAC4200.048.6683961
ATCTCGT4250.048.5192637
ACGAGAC4300.048.3245922
TATACAC4300.048.3160673
CATCTCG4200.048.24426336
CTACATC4200.048.13305733
ATCTCCG4350.047.7691310
TCTACAT4300.047.7470232
TACATCT4250.047.60899434
ACCTCTC4400.047.26796727
CTTGAAA4500.047.2611257
CACGAGA4400.047.226321
CCGTCTT4500.047.2045547
CCCACGA4350.046.9818919
CGAGCCC4400.046.44391315
GTATGCC4600.045.9135942
Produced by FastQC (version 0.11.5)