FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004688868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences567427
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46140.8131442458677504No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32830.5785766274780721No Hit
GCCTTGGGCTGACCCAGGACGGTCAGTCTGGTCCCCCCGCCGAATGGGATACCGTACGAGCTTGCAAAGGAGCAG26640.46948770502637344No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTGTCTGCCTTCCAG23400.4123878490096523No Hit
GTCCTGGGCCCAGGCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG23170.4083344641689592No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21660.381723111519191No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCTCTGGAAGCAGCGATGACATT19080.3362547076540242No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18410.32444702137896153No Hit
GATTATTACTGCTGCTCCTTTGCAAGCTCGTACGGTATCCCATTCGGCGGGGGGACCAGACTGACCGTCCTGGGT18340.32321338251440274No Hit
CTCTAAGAGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCT18160.32004116829125157No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17560.3094671208807477No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17240.3038276289284789No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17230.3036513948049705No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGTCTGGTCCCCC15840.27915485163730314No Hit
GGGCACAGGGTCCTGGGCCCAGGCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC15820.2788023833902863No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15390.2712243160794252No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG14860.26188390753348006No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGTTTTGTTTGGAGGGTGTGGTGGTCTCCA13420.23650619374827067No Hit
GTAATAATCAGCTTCATCGTCAGGTCGGAGCCCAGAGACGGTCAGGGAGG13150.23174787241354394No Hit
GTCTGGTCCCCCCGCCGAATGGGATACCGTACGAGCTTGCAAAGGAGCAG12910.22751825344934237No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12710.2239935709791744No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGTTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12570.22152629325005685No Hit
GTCAGTCACCATCTCCTGCTCTGGAAGCAGCGATGACATTGGTGCTTCTA12500.22029265438549805No Hit
CCACCACACCCTCCAAACAAAACAACAACAAGTACGCGGCCAGCAGCTAT12350.21764914253287207No Hit
TCTCTGGGCTCCGACCTGACGATGAAGCTGATTATTACTGCTGCTCCTTT12220.2153580989272629No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12140.21394822593919569No Hit
CTCTTAGAGACCTCAAAAATGATGAGTTTGGGGGCTTTGCCTGGATATTG11570.20390288089921696No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG11510.20284547615816662No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11270.19861585719396502No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11160.19667728183537267No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10730.1890992145245115No Hit
GTATCCCATTCGGCGGGGGGACCAGACTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC10670.18804180978346113No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGGCTGCCCTGACTCAGC10600.18680817091890234No Hit
ATGGGATACCGTACGAGCTTGCAAAGGAGCAGCAGTAATAATCAGCTTCA10200.1797588059785664No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC10170.1792301036080412No Hit
GGGCTGCTGTCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10150.1788776353610244No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10050.17711529412594043No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9750.17182827042068846No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9560.1684798220740289No Hit
CTCCTGCTCTGGAAGCAGCGATGACATTGGTGCTTCTAACTTTGTCTCCTGGTACCAACAATATCCAGGCAAAGC9400.16566007609789452No Hit
GTTTGGGGGCTTTGCCTGGATATTGTTGGTACCAGGAGACAAAGTTAGAA9280.16354526661579374No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9220.16248786187474337No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT9180.16178292538070976No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC9180.16178292538070976No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9060.159668115898609No Hit
ACAATATCCAGGCAAAGCCCCCAAACTCATCATTTTTGAGGTCTCTAAGAGGCCCTCAGGGGTCCCTGATCGCTT9040.15931564765159217No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8760.15438109219335702No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGGCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT8750.15420485806984863No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCTCTGGAAGCAGCGATGACATTGGTG8620.15191381446423946No Hit
CCCCTGAGGGCCTCTTAGAGACCTCAAAAATGATGAGTTTGGGGGCTTTGCCTGGATATTGTTGGTACCAGGAGA8570.15103264384669746No Hit
CCGTACGAGCTTGCAAAGGAGCAGCAGTAATAATCAGCTTCATCGTCAGG8560.15085640972318906No Hit
GGACCAGACTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG8560.15085640972318906No Hit
GACCCAGGACGGTCAGTCTGGTCCCCCCGCCGAATGGGATACCGTACGAGCTTGCAAAGGAGCAGCAGTAATAAT8490.14962277085863027No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGGCTGCCCTGACT8470.14927030261161348No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8420.14838913199407147No Hit
CTCGTACGGTATCCCATTCGGCGGGGGGACCAGACTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCA8410.1482128978705631No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC8380.1476841955000379No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC8290.1460980883884623No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT8110.14292587416531113No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGTCTGGTCCCCCCGCCGAATGGGATAC7840.13816755283058438No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG7810.1376388504600592No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7660.1349953386074332No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG7460.13147065613726522No Hit
CCCTGACCGTCTCTGGGCTCCGACCTGACGATGAAGCTGATTATTACTGC7070.1245975253204377No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGGCTGCCCTGACTCAGCCT6990.12318765233237051No Hit
GGCCCAGGCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGAC6950.1224827158383369No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC6900.12160154522079493No Hit
CTCCCTGACCGTCTCTGGGCTCCGACCTGACGATGAAGCTGATTATTACT6860.12089660872676132No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA6770.11931050161518575No Hit
GTCTCTAAGAGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGT6730.11860556512115214No Hit
GCTCTGGAAGCAGCGATGACATTGGTGCTTCTAACTTTGTCTCCTGGTAC6730.11860556512115214No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG6690.11790062862711856No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAACAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC6630.11684322388606817No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG6620.11666698976255978No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGTCTGGTC6400.11278983904537501No Hit
CAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAACAACAACAAGTACGCGGCCAGCAG6390.1126136049218666No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCGAC6390.1126136049218666No Hit
ACCCAGGACGGTCAGTCTGGTCCCCCCGCCGAATGGGATACCGTACGAGC6300.11102749781029102No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC6270.11049879543976582No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTGTCTGCCTTCCAGGCCACTGTCA6260.11032256131625744No Hit
GCCCCCAAACTCATCATTTTTGAGGTCTCTAAGAGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGT6130.10803151771064823No Hit
GGCCTGGAAGGCAGACAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA6080.10715034709310625No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTGTCTGCCTTCCAGGCCACTGTCACGGCTC5940.10468306936398868No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG5890.10380189874644669No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT5880.10362566462293829No Hit
GGATATTGTTGGTACCAGGAGACAAAGTTAGAAGCACCAATGTCATCGCTGCTTCCAGAGCAGGAGATGGTGA5810.1023920257583795No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG5780.1018633233878543No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC5770.1016870892643459No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGG5760.10151085514083749No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC5680.1001009821527703No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)