FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences626132
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCAACAATAAGAGTCTTTTAAGTTGGTACCAGCAGAA65641.0483412443382545No Hit
CTCCTAAGGTGATCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT53040.8471057221161032No Hit
CCTTAGGAGGCTGCCCTGGTTTCTGCTGGTACCAACTTAAAAGACTCTTATTGTTGGAGCTGTATAAAACACT52940.8455086147968799No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40070.6399609028128254No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37070.5920476832361228No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAATCTGGAGA32840.524490043632972No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30050.4799307494266385No Hit
GTTTATTACTGTCACCAATGTCACGCTATTCCGTTCACCTTCGGCCAAGGGACACGACTGGAGATGAAACGAA29200.46635533721323935No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27810.44415554547603375No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24680.3941660863843407No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23430.3742022448940479No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22510.3595088575571924No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22240.3551966677952892No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21710.346731999003405No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21150.3377881980157539No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20910.33395514044961766No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20790.3320386116665496No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20720.33092063654309317No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20220.322935099946976No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC19490.31127621651664505No Hit
GTAATAAACTGCCACATCCTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA18970.3029712584566832No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAGGATGTGGCAGTTTATTACTGTCACCAATGTCACGCTATT18740.2992979116224694No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC17610.2812505989152447No Hit
GGACACGACTGGAGATGAAACGAACTGTGGCTGCACCATCTGTCTTCATC17530.279972913059866No Hit
GAATAGCGTGACATTGGTGACAGTAATAAACTGCCACATCCTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAAT17440.27853551647256486No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATCTCCAGTCGTGTCC17340.27693840915334145No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16750.26751547596992326No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16640.2657586579187775No Hit
ACGCTATTCCGTTCACCTTCGGCCAAGGGACACGACTGGAGATGAAACGA16260.25968965010572853No Hit
GCCTGAGGATGTGGCAGTTTATTACTGTCACCAATGTCACGCTATTCCGTTCACCTTCGGCCAAGGGACACGA16180.25841196425034973No Hit
CAATAAGAGTCTTTTAAGTTGGTACCAGCAGAAACCAGGGCAGCCTCCTAAGGTGATCATTTACTGGGCATCTAC16120.2574536998588157No Hit
GTGCAGCCACAGTTCGTTTCATCTCCAGTCGTGTCCCTTGGCCGAAGGTG15580.24882932033500924No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15310.24451713057310598No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14780.23605246178122186No Hit
ATTCCGTTCACCTTCGGCCAAGGGACACGACTGGAGATGAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC14590.23301795787469734No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14080.2248727105466579No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13580.21688717395054075No Hit
GCAGAAACCAGGGCAGCCTCCTAAGGTGATCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATT13260.2117764305290258No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13030.20810308369481195No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12950.2068253978394332No Hit
GTCGTGTCCCTTGGCCGAAGGTGAACGGAATAGCGTGACATTGGTGACAGTAATAAACTGCCACATCCTCAGG12790.20427002612867576No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12720.20315205100521933No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12550.2004369685625395No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC12350.19724275392409268No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12210.19500680367717987No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT12010.19181258903873305No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGATTCCCTGGCTGTGTCTCTGGGCGAGAGGG11950.19085432464719898No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGATCACCTTAGGAGGCTGCCCTGGTTTCTGCTGGTA11730.18734068854490749No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTT11680.18654213488529575No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11620.1855838704937617No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11540.18430618463838297No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11490.18350763097877126No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT11370.18159110219570312No Hit
TCCTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC11220.179195441216868No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11110.17743862316572226No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11090.17711920170187756No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10990.17552209438265412No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10990.17552209438265412No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10930.17456382999112008No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10860.17344585486766367No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10680.17057106169306155No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG10620.16961279730152745No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10450.16689771485884763No Hit
CACCATCAGCAGCCTGCAGCCTGAGGATGTGGCAGTTTATTACTGTCACCAATGTCACGCTATTCCGTTCACCTT10420.16641858266308063No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAGGAT10290.16434234314809018No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGATTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG10220.16322436802463378No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10120.16162726070541036No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10010.1598704426542646No Hit
CGACTGGAGATGAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT9990.15955102119041992No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC9910.15827333533504118No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9750.1557179636242837No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG9750.1557179636242837No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9740.15555825289236136No Hit
CCTTCGGCCAAGGGACACGACTGGAGATGAAACGAACTGTGGCTGCACCA9710.15507912069659432No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAGGATG9680.1545999885008273No Hit
AACCAGGGCAGCCTCCTAAGGTGATCATTTACTGGGCATCTACCCGGGAA9630.1538014348412156No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9540.1523640382539145No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9410.15028779873892406No Hit
CAGTAATAAACTGCCACATCCTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA9390.1499683772750794No Hit
TTATTACTGTCACCAATGTCACGCTATTCCGTTCACCTTCGGCCAAGGGA9310.14869069141970065No Hit
GGTTTCTGCTGGTACCAACTTAAAAGACTCTTATTGTTGGAGCTGTATAA9310.14869069141970065No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT9280.14821155922393361No Hit
CCAGATTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAG9280.14821155922393361No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9280.14821155922393361No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9240.14757271629624424No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA9160.1462950304408655No Hit
CACCAATGTCACGCTATTCCGTTCACCTTCGGCCAAGGGACACGACTGGA9000.14373965873010802No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8950.14294110507049632No Hit
GAACGGAATAGCGTGACATTGGTGACAGTAATAAACTGCCACATCCTCAG8910.14230226214280695No Hit
ATGTCACGCTATTCCGTTCACCTTCGGCCAAGGGACACGACTGGAGATGAAACGAACTGTGGCTGCACCAT8870.14166341921511758No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT8720.13926775823628243No Hit
CTCTTATTGTTGGAGCTGTATAAAACACTCTGGCTGGACTTGCAGTTGAT8630.13783036164898135No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8610.13751094018513668No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG8590.13719151872129198No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8580.13703180798936967No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG8430.13463614701053453No Hit
GTAAATGATCACCTTAGGAGGCTGCCCTGGTTTCTGCTGGTACCAACTTA8380.13383759335092282No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8300.13255990749554408No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC8140.1300045357847866No Hit
CTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAACAATAAGAGTCTTT8100.12936569285709723No Hit
CTTTTAAGTTGGTACCAGCAGAAACCAGGGCAGCCTCCTAAGGTGATCAT7880.12585205675480568No Hit
GGATGTGGCAGTTTATTACTGTCACCAATGTCACGCTATTCCGTTCACCT7790.12441466016750462No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7670.12249813138443649No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC7550.1205816026013684No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA7550.1205816026013684No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7510.11994275967367903No Hit
GTCTCCAGATTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA7430.1186650738183003No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT7420.11850536308637795No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA7360.11754709869484391No Hit
GTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGG7190.11483201625216409No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA7180.11467230552024174No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC7160.11435288405639707No Hit
GATCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT7100.113394619664863No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT7050.1125960660052513No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7000.11179751234563957No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC6960.1111586694179502No Hit
ACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC6890.11004069429449383No Hit
ACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAACAATAA6830.10908242990295976No Hit
GCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAG6800.10860329770719272No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATCTCCAGTCGTGTCCCTTGGC6790.10844358697527037No Hit
ACATTGGTGACAGTAATAAACTGCCACATCCTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGA6740.10764503331565868No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA6700.10700619038796931No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG6700.10700619038796931No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG6600.10540908306874588No Hit
GTGATGACCCAGTCTCCAGATTCCCTGGCTGTGTCTCTGGGCGAGAGGGC6570.10492995087297886No Hit
CATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATG6530.1042911079452895No Hit
GTAATAGACGGCAGTGTCCTCAGCCTTCAGGCTGCTGATCTGTAGATACG6460.10317313282183309No Hit
AAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTC6460.10317313282183309No Hit
GATTCACTTTATTTATTCATTCTCCTCCAACATTAGCATAATTAAAGCCA6430.10269400062606607No Hit
GCCTGCAGCCTGAGGATGTGGCAGTTTATTACTGTCACCAATGTCACGCTATTCCGTTCACCTTCGGCCAAGGGA6420.10253428989414372No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT6340.10125660403876499No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT6320.10093718257492029No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)