FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences626132
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCAACAATAAGAGTCTTTTAAGTTGGTACCAGCAGAA58340.9317524100349447No Hit
CTCCTAAGGTGATCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT48240.770444570793379No Hit
CCTTAGGAGGCTGCCCTGGTTTCTGCTGGTACCAACTTAAAAGACTCTTATTGTTGGAGCTGTATAAAACACT48030.7670906454230099No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41840.66822970236308No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31440.502130541163844No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31240.49893632652539716No Hit
GTTTATTACTGTCACCAATGTCACGCTATTCCGTTCACCTTCGGCCAAGGGACACGACTGGAGATGAAACGAA29020.4634805440386372No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28690.45821008988519996No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAATCTGGAGA27650.4416001737652764No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25960.41460906007040044No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24480.3909718717458938No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24060.3842640210051555No Hit
GTAATAAACTGCCACATCCTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA22300.35615493218682326No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21410.3419406770457348No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20810.33235803313039425No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20270.32373365360658773No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC20110.3211782818958303No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19840.316866092133927No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19410.3099985306612663No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19200.3066446052908971No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19020.30376981211629495No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAGGATGTGGCAGTTTATTACTGTCACCAATGTCACGCTATT18360.2932289038094204No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18080.2887570033155948No Hit
GAATAGCGTGACATTGGTGACAGTAATAAACTGCCACATCCTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAAT17760.2836462598940799No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17150.27390390524681696No Hit
GGACACGACTGGAGATGAAACGAACTGTGGCTGCACCATCTGTCTTCATC16890.26975142621683607No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATCTCCAGTCGTGTCC16830.268793161825302No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC16140.2577731213226604No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16140.2577731213226604No Hit
GCCTGAGGATGTGGCAGTTTATTACTGTCACCAATGTCACGCTATTCCGTTCACCTTCGGCCAAGGGACACGA16060.25649543546728165No Hit
ACGCTATTCCGTTCACCTTCGGCCAAGGGACACGACTGGAGATGAAACGA15850.25314151009691244No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15840.2529817993649901No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15600.24914874179885393No Hit
GTGCAGCCACAGTTCGTTTCATCTCCAGTCGTGTCCCTTGGCCGAAGGTG15450.24675308082001876No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG14950.23876754422390167No Hit
TCCTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC14760.23573304031737716No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14460.23094171835970687No Hit
ATTCCGTTCACCTTCGGCCAAGGGACACGACTGGAGATGAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC14330.22886547884471645No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT13750.2196022563932206No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12700.20283262954137465No Hit
GTCGTGTCCCTTGGCCGAAGGTGAACGGAATAGCGTGACATTGGTGACAGTAATAAACTGCCACATCCTCAGG12610.20139523295407358No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12610.20139523295407358No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12300.19644420026448095No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT12090.1930902748941118No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12060.19261114269834476No Hit
CAATAAGAGTCTTTTAAGTTGGTACCAGCAGAAACCAGGGCAGCCTCCTAAGGTGATCATTTACTGGGCATCTAC12050.19245143196642242No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11970.19117374611104368No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11840.18909750659605323No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11800.18845866366836386No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC11440.18270907731915953No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11340.18111196999993612No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11330.18095225926801375No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11300.18047312707224675No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG11190.178716309021101No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11040.17632064804226585No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGATCACCTTAGGAGGCTGCCCTGGTTTCTGCTGGTA10960.1750429621868871No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10950.17488325145496478No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10930.17456382999112008No Hit
GCAGAAACCAGGGCAGCCTCCTAAGGTGATCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATT10880.17376527633150837No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGATTCCCTGGCTGTGTCTCTGGGCGAGAGGG10790.1723278797442073No Hit
CAGTAATAAACTGCCACATCCTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA10770.1720084582803626No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10550.16849482217807107No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10460.16705742559077No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTT10450.16689771485884763No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10230.16338407875655614No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAGGAT10200.1629049465607891No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10020.16003015338618692No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9920.1584330460669635No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAGGATG9910.15827333533504118No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9810.15667622801581774No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9480.15140577386238047No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9470.1512460631304581No Hit
CACCATCAGCAGCCTGCAGCCTGAGGATGTGGCAGTTTATTACTGTCACCAATGTCACGCTATTCCGTTCACCTT9460.15108635239853577No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9440.1507669309346911No Hit
CGACTGGAGATGAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT9230.14741300556432188No Hit
GAACGGAATAGCGTGACATTGGTGACAGTAATAAACTGCCACATCCTCAG9200.14693387336855487No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9090.14517705531740913No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT9090.14517705531740913No Hit
CCTTCGGCCAAGGGACACGACTGGAGATGAAACGAACTGTGGCTGCACCA9080.14501734458548676No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8950.14294110507049632No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8820.14086486555550587No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8790.14038573335973883No Hit
TTATTACTGTCACCAATGTCACGCTATTCCGTTCACCTTCGGCCAAGGGA8720.13926775823628243No Hit
GGTTTCTGCTGGTACCAACTTAAAAGACTCTTATTGTTGGAGCTGTATAA8620.13767065091705902No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8520.13607354359783558No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8480.13543470067014624No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8470.1352749899382239No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA8440.13479585774245686No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8390.13399730408284516No Hit
ATGTCACGCTATTCCGTTCACCTTCGGCCAAGGGACACGACTGGAGATGAAACGAACTGTGGCTGCACCAT8350.13335846115515576No Hit
CACCAATGTCACGCTATTCCGTTCACCTTCGGCCAAGGGACACGACTGGA8170.13048366798055364No Hit
GTAAATGATCACCTTAGGAGGCTGCCCTGGTTTCTGCTGGTACCAACTTA8150.13016424651670894No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC8110.12952540358901957No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC7920.12649089968249505No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGATTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG7910.12633118895057271No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG7870.12569234602288337No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG7720.12329668504404823No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT7630.12185928845674715No Hit
CTCTTATTGTTGGAGCTGTATAAAACACTCTGGCTGGACTTGCAGTTGAT7600.12138015626098012No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC7590.12122044552905778No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT7560.12074131333329075No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7520.12010247040560137No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7380.11786652015868858No Hit
CCAGATTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAG7260.11594999137562048No Hit
AACCAGGGCAGCCTCCTAAGGTGATCATTTACTGGGCATCTACCCGGGAA7240.11563056991177578No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG7140.11403346259255237No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG7100.113394619664863No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG7070.11291548746909597No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT7010.11195722307756192No Hit
GTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGG6970.11131838014987255No Hit
GGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGATTCC6960.1111586694179502No Hit
CTTTTAAGTTGGTACCAGCAGAAACCAGGGCAGCCTCCTAAGGTGATCAT6940.11083924795410552No Hit
GTCTCCAGATTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA6940.11083924795410552No Hit
GGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCC6920.11051982649026085No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA6890.11004069429449383No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT6840.1092421406348821No Hit
GGATGTGGCAGTTTATTACTGTCACCAATGTCACGCTATTCCGTTCACCT6830.10908242990295976No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA6790.10844358697527037No Hit
GCCTGCAGCCTGAGGATGTGGCAGTTTATTACTGTCACCAATGTCACGCTATTCCGTTCACCTTCGGCCAAGGGA6770.1081241655114257No Hit
GTAATAGACGGCAGTGTCCTCAGCCTTCAGGCTGCTGATCTGTAGATACG6750.10780474404758103No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC6720.10732561185181401No Hit
ACATTGGTGACAGTAATAAACTGCCACATCCTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGA6720.10732561185181401No Hit
GGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACA6690.10684647965604696No Hit
AAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTC6690.10684647965604696No Hit
GGCCGAAGGTGAACGGAATAGCGTGACATTGGTGACAGTAATAAACTGCC6680.10668676892412463No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC6680.10668676892412463No Hit
GATCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT6670.10652705819220229No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT6530.1042911079452895No Hit
GCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAG6420.10253428989414372No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG6400.10221486843029905No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATCTCCAGTCGTGTCCCTTGGC6330.10109689330684264No Hit
GAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGG6290.10045805037915327No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGG150.001958904372.27914462
CATTGGG206.8515605E-572.0881461
GTTCGCC150.002550516567.62623623
ACCTAAC500.067.5992051
TTTCGAA150.002556182267.588416
CTAATGG300.00265187866.95692470
CCTAACG600.061.936232
CTAACGC600.061.9114953
ATTCGCA350.004883734557.39165570
CAGTATC252.8398732E-454.079361
CATTCGC250.00673942452.9092469
TACTCGC200.00787101850.87426827
ATACTCG200.00790601950.817226
CTATACC200.00800666150.6548584
ATAAGAT200.00800666150.6548583
ACAGGTT651.362605E-841.5995061
GCCGTTC350.001490248138.6281131
CCTATAC350.001496699338.5941773
ATGCATG901.1934624E-437.19829670
GACAGGT1100.036.872291
Produced by FastQC (version 0.11.5)