FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1235972
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC41770.33795263970381206No Hit
GTCCTGGGCCCAGTCTGTCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG33970.27484441395112513No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT31290.25316107484635575No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATAACACCCCGCTGCTGCCTGCATATGAGCTG30990.2507338353943293No Hit
GTCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC30620.24774024007016338No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG30540.24709297621628967No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29720.24045852171408413No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28970.23439042308401806No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATAACACCCCGCTGCTG27470.22225422582388596No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC26240.21230254407057764No Hit
CTCCTGTACAGCCTCTGGATTCACTTTCAGTGACTGCTACATGACCTGGATTCGCCAGGTTCCAGGGAAGGGG26110.21125074030803287No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24480.19806273928535595No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG23500.190133757075403No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23190.18762560964164238No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA22860.18495564624441332No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT22380.18107206312117102No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21970.17775483587006827No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGTCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC20350.16464774282912556No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20280.1640813869569861No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC20160.16311049117617551No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT20150.1630295831944413No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19330.15639512869223574No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19280.15599058878356467No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG19190.15526241694795676No Hit
GTGTTATTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG19030.15396788924020932No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18640.15081247795257496No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17930.14506801124944577No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCA17900.14482528730424316No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17770.14377348354169836No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT17540.14191259996181144No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG17520.14175078399834298No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT17480.14142715207140613No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17130.13859537271070865No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16960.137219937021227No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC16650.13471178958746638No Hit
TCTCTGGGCTCCAGGCTGACGATGAGGCTTATTATTACTGCAGCTCATAT16640.13463088160573217No Hit
AGCCTGGAGGGTCCCTGAGACTCTCCTGTACAGCCTCTGGATTCACTTTC16560.13398361775185846No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16530.13374089380665583No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16380.1325272740806426No Hit
TAATAAGCCTCATCGTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGACAGACTTGGAGCCAGAGAAG16180.13090911444595832No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGTCCTGACTCAGC15860.12832005903046348No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAA15620.12637826746884234No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGTCCTGACTCAGCCTCCCTCCGCGTCCGGGT15580.1260546355419055No Hit
AGTCTGTCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA15490.12532646370629755No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC15410.12467919985242384No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15150.12257559232733428No Hit
CTTAATGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGTT14780.11958199700316835No Hit
GTCATTAAGAGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGTCAACACGGCCTCCCTGACCGT14690.11885382516756042No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14490.11723566553287616No Hit
GGCTTATTATTACTGCAGCTCATATGCAGGCAGCAGCGGGGTGTTATTCG14130.11432297819044443No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGTCCTGACT14100.1140802542452418No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT14060.11375662231830495No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC13980.11310935846443122No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13910.11254300259229175No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC13810.11173392277494959No Hit
CCCCTGAGGGCCTCTTAATGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA13560.10971122323159424No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13480.10906395937772054No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC13110.1060703640535546No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATAACACCCCGCTGCTGC13000.10518037625447825No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT12770.10331949267459134No Hit
CTCCAGGGACAACGCCAAGAACTCACTGTATCTGCATATGAACACCGTGAGAGCCGAGGACACGGCTTTTTATTA12710.10283404478418606No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12550.10153951707643863No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGACAGACTTGGAGCCAGAGAAG12540.10145860909470442No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT12360.10000226542348856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1650.052.19854740
TCTCGTA1650.047.8398538
CGTATGC1850.046.64362741
CGTAGAG2000.045.8736828
GACGTAG2000.045.82724826
AGACGTA2050.044.70046225
ATCTCGT1800.043.78508837
GGTATCA9050.043.502231
TATACAC2350.043.3109133
AATCTCG1850.042.5564936
CGAGACG2200.041.63753523
GTATGCC2100.041.18236542
CTTATAC2250.040.727091
ATGCCGT2150.040.35283344
ACGTAGA2300.039.87238327
GCCGTCT2150.038.89972746
ACGAGAC2450.038.7704422
GTATCAA23350.037.2096181
AGGAATC2400.036.95750433
TGCCGTC2150.035.5797145
Produced by FastQC (version 0.11.5)