FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1235972
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC39190.3170783804163848No Hit
GTCCTGGGCCCAGTCTGTCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG33150.26820995944891957No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32250.2609282410928403No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG31970.2586628176042823No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATAACACCCCGCTGCTGCCTGCATATGAGCTG31920.2582582776956112No Hit
GTCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC29750.24070124565928677No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28480.2304259319790416No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATAACACCCCGCTGCTG28060.2270277967462046No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25400.20550627360490367No Hit
CTCCTGTACAGCCTCTGGATTCACTTTCAGTGACTGCTACATGACCTGGATTCGCCAGGTTCCAGGGAAGGGG25370.205263549659701No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC25100.20307903415287726No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA24780.20048997873738242No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24610.19911454304790077No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23660.1914282847831504No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23120.18705925376950286No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGTCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC23000.1860883579886923No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22660.18333748660972904No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT22170.17937299550475255No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG20700.16747952218982307No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC20420.16521409870126508No Hit
GTGTTATTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG20210.16351503108484658No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20150.1630295831944413No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20120.16278685924923866No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC19800.1601978038337438No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG19200.15534332492969097No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC19070.15429152116714617No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17370.1405371642723298No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17240.139485360509785No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCA16920.13689630509429015No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16890.13665358114908752No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16730.13535905344134008No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT16610.13438815766052953No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16600.13430724967879532No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16520.1336599858249216No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15640.12654008343231077No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGTCCTGACTCAGC15640.12654008343231077No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15520.1255691876515002No Hit
GAATAACACCCCGCTGCTGCCTGCATATGAGCTGCAGTAATAATAAGCCT15480.12524555572456333No Hit
TCTCTGGGCTCCAGGCTGACGATGAGGCTTATTATTACTGCAGCTCATAT15090.122090144436929No Hit
AGCCTGGAGGGTCCCTGAGACTCTCCTGTACAGCCTCTGGATTCACTTTC15040.12168560452825793No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT14860.12022926085704207No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAA14560.11780202140501564No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT14240.1152129659895208No Hit
GGCTTATTATTACTGCAGCTCATATGCAGGCAGCAGCGGGGTGTTATTCG14150.11448479415391286No Hit
GTCATTAAGAGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGTCAACACGGCCTCCCTGACCGT13900.11246209461055752No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC13830.11189573873841802No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGTCCTGACTCAGCCTCCCTCCGCGTCCGGGT13770.11141029084801274No Hit
CCCCTGAGGGCCTCTTAATGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA13670.1106012110306706No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT13570.10979213121332847No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13490.10914486735945474No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13440.10874032745078369No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13390.10833578754211261No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC13360.10809306359690996No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC13350.10801215561517576No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC13330.10785033965170733No Hit
CTTAATGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGTT13320.1077694316699731No Hit
AGTCTGTCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA13160.10647490396222568No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13020.10534219221794668No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC12910.10445220441887032No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGTCCTGACT12890.1042903884554019No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATAACACCCCGCTGCTGC12760.10323858469285711No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12600.10194405698510971No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGACG252.5358074E-455.33783742
TAGTACC306.882407E-445.19889
GGTATCA10900.044.507341
GGATGGG12150.040.38749770
AGTAAGA951.4796417E-435.87047270
AATGGTA3300.035.10958570
GTGGTAT3150.034.4636731
GTATCAA25850.034.2532731
TGGTATC3350.032.3930242
TATCAAC28000.031.2469772
ACGGGTA1209.458745E-1131.07920316
CGGACGG450.004529046430.7953643
ATCAACG28600.030.4704043
TCAACGC28900.030.2616394
GTGAGCG6100.030.1664870
AACGCAG29550.029.3677486
CAACGCA29650.029.2686985
TCCGAGA7350.028.74518270
AGGATGG11350.028.59559269
TCAGGTC9150.028.52595712
Produced by FastQC (version 0.11.5)