FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences942363
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43160.45799760814038754No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40630.43115020432678275No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA33210.3524119686362898No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29690.3150590589825789No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26750.2838608901240817No Hit
GTCTCCAGACACCCTGTCTTTGTCTGCAGGGGAGAGAGCCACCCTCTCCT25720.27293091940154696No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25310.2685801543566545No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAACCTAGAGCCTGAAGATT24870.26391104064994064No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24500.2599847404874767No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24330.2581807647371554No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24230.2571196025310841No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24140.25616455654561987No Hit
CTCCTGCAGGGCCAGTCAGAACGGTCAGAATGGTAAAATCTACGTAGCCTGGTACCAACAGAAACCTGGCCAGG23420.2485241886619063No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22570.23950430991029997No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21660.22984773383505083No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21060.22348076059862282No Hit
GTTTATTACTGTCAGCAGCGTAGCACCTGGACTCCAGGGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAA20940.2222073659513372No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20740.22008504153919456No Hit
CTCCCAGACTCCTCATTTATGATGCATCCAACAGGGCCACTGGCATCCCA20710.21976669287737316No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT19960.21180797633183818No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19630.20830614105180278No Hit
GCGTAGCACCTGGACTCCAGGGATCACCTTCGGCCAAGGGACACGACTGG18200.1931315215049827No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGATCCCTGGAGT18070.19175201063708996No Hit
GTCTTTGTCTGCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA17810.18899298890130448No Hit
GACTTCACTCTCACCATCAGCAACCTAGAGCCTGAAGATTTTGCAGTTTA17400.18464222385641202No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17330.1838994103121621No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGTTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17280.18336882920912642No Hit
GTCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGCTACGTAGATTTTACCATTCTGACCGTTCTGACTGGCC16760.17785078573755547No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA16640.17657739109026988No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16180.17169604494234175No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15810.16776974477987783No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15650.16607188525016367No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT15640.16596576902955654No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC15390.1633128635143782No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT15310.16246393374952114No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15150.16076607421980701No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGATCCCTGGAGTCCAGGTGCTACG14460.1534440549979148No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14330.1520645441300221No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14120.14983610349727228No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13710.14548533845237982No Hit
ACAGAAACCTGGCCAGGCTCCCAGACTCCTCATTTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTT13070.13869390033352327No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA13000.13795108678927334No Hit
GCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGACTCCTCATTTATGATGCATCCAACAGGGCCACTGGCATC12940.13731438946563054No Hit
GTGCTACGCTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGTTGCTGATGGTGAGAGTGAAGTCTGTC12510.13275139197952382No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC12470.1323269270970953No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12410.1316902297734525No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12310.13062906756738116No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12210.12956790536130983No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAACGGTCAGAATGGTAAAATCTACGTAGCCTGGTACCAACAGAAAC11970.1270211160667386No Hit
CAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAA11950.12680888362552437No Hit
GTGTTGACACAGTCTCCAGACACCCTGTCTTTGTCTGCAGGGGAGAGAGC11930.12659665118431007No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11800.12521714031641734No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11580.12288258346306043No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11430.12129084015395342No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11370.1206541428303106No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11350.12044191038909635No Hit
CTCCAGACACCCTGTCTTTGTCTGCAGGGGAGAGAGCCACCCTCTCCTGC11350.12044191038909635No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11240.11927463196241789No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11200.11885016707998936No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11170.11853181841816796No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11090.11768288865331089No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCCCTGGAGTCCAGGTGCTACGCTGCTGACAGTAATAAACTGCAAAATCTT11070.11747065621209661No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11060.11736453999148949No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11040.11715230755027521No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11000.11672784266784668No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10990.11662172644723953No Hit
CAGTAATAAACTGCAAAATCTTCAGGCTCTAGGTTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA10940.11609114534420388No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10930.11598502912359675No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10900.11566668046177535No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT10890.11556056424116821No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10810.11471163447631114No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10800.11460551825570402No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10770.1142871695938826No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10740.1139688209320612No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC10740.1139688209320612No Hit
GGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAACGGTCAGAATGGTA10730.11386270471145407No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10670.11322600738781127No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10570.11216484518173996No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAACC10420.11057310187263295No Hit
CCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTCTC10170.1079201963574546No Hit
CCCAGATACCACCGGAGAAATTGTGTTGACACAGTCTCCAGACACCCTGTCTTTGTCTGCAGGGGAGAGAGCCAC10090.10707126659259755No Hit
ACCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCACC10070.10685903415138327No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGAGA9990.10601010438652621No Hit
CCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCACCT9980.10590398816591909No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9660.1025082691064908No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9630.10218992044466942No Hit
CCCTGTCTTTGTCTGCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGT9530.10112875823859808No Hit
ACCCTCTCCTGCAGGGCCAGTCAGAACGGTCAGAATGGTAAAATCTACGTAGCCTGGTACCAACAGAAACCTGGC9440.10017371225313387No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9440.10017371225313387No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAATCG200.0071929952.04874844
GGTATCA10100.047.160871
TTAACGA306.178625E-446.19802543
TCGCACA306.76587E-445.354632
TCGTATG2600.043.80175440
CGTATGC2750.041.48851441
TATACAC2800.041.3051153
ATGCCGT2900.040.68177844
TTCTAGT650.001332663239.51500770
ACGAGAC2850.039.40783322
CGTCTGA350.001430349538.95395728
CGAGACA3000.037.44340523
ATCTCGT2600.037.01168437
CTTATAC3250.036.6403661
CACGAGA3100.036.22593321
GGCAATC2650.036.13702433
GCAATCT2950.034.8235334
GTATGCC3400.034.6167142
TGTACGA400.00255343534.6167142
GTATCAA25500.034.5568921
Produced by FastQC (version 0.11.5)