FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences942363
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44020.46712360311260104No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35300.3745902587431807No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31930.3388290923985768No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31070.32970309742636333No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA30720.3259890297051136No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAACCTAGAGCCTGAAGATT25520.2708085949894043No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25250.2679434570330117No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24740.26253152978204786No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24560.2606214378111195No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24030.2549972781189414No Hit
GTCTCCAGACACCCTGTCTTTGTCTGCAGGGGAGAGAGCCACCCTCTCCT23620.25064651307404895No Hit
CTCCTGCAGGGCCAGTCAGAACGGTCAGAATGGTAAAATCTACGTAGCCTGGTACCAACAGAAACCTGGCCAGG22690.24077770455758554No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21580.22899880407019377No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21570.2288926878495866No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT20550.21806883334765903No Hit
GTTTATTACTGTCAGCAGCGTAGCACCTGGACTCCAGGGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAA20480.2173260198034091No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20150.2138241845233737No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19590.20788167616937422No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19500.20692663018391003No Hit
GTCTTTGTCTGCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA19060.20225751647719617No Hit
CTCCCAGACTCCTCATTTATGATGCATCCAACAGGGCCACTGGCATCCCA18980.2014085867123391No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18790.19939237852080355No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18740.19886179741776788No Hit
GCGTAGCACCTGGACTCCAGGGATCACCTTCGGCCAAGGGACACGACTGG17680.18761347803341177No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17550.18623396716551902No Hit
GTGCTACGCTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGTTGCTGATGGTGAGAGTGAAGTCTGTC17290.18347494542973355No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGTTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17270.1832627129885193No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17110.18156485345880516No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17020.18060980747334093No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGATCCCTGGAGT16520.17530399644298428No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA16500.17509176400177No Hit
GTCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGCTACGTAGATTTTACCATTCTGACCGTTCTGACTGGCC16270.17265109092780595No Hit
GACTTCACTCTCACCATCAGCAACCTAGAGCCTGAAGATTTTGCAGTTTA15900.168724790765342No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15560.1651168392646995No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGATCCCTGGAGTCCAGGTGCTACG15070.15991714445494995No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14780.15683977405734306No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT14570.15461133342459327No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC14170.15036668460030794No Hit
GCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGACTCCTCATTTATGATGCATCCAACAGGGCCACTGGCATC13350.14166515451052303No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13090.13890613277473754No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13050.138481667892309No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12910.13699604080380914No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC12720.13497983261227361No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12520.13285750820013095No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12490.13253915953830955No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12410.1316902297734525No Hit
ACAGAAACCTGGCCAGGCTCCCAGACTCCTCATTTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTT12220.12967402158191693No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCCCTGGAGTCCAGGTGCTACGCTGCTGACAGTAATAAACTGCAAAATCTT12090.12829451071402423No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA12080.12818839449341707No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11770.12489879165459594No Hit
GTGTTGACACAGTCTCCAGACACCCTGTCTTTGTCTGCAGGGGAGAGAGC11560.12267035102184615No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11490.12192753747759622No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11410.12107860771273915No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11410.12107860771273915No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11390.12086637527152488No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11120.11800123731513228No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAACGGTCAGAATGGTAAAATCTACGTAGCCTGGTACCAACAGAAAC11100.117789004873918No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11050.11725842377088234No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11040.11715230755027521No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10690.11343823982902554No Hit
CAGTAATAAACTGCAAAATCTTCAGGCTCTAGGTTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA10650.11301377494659702No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10640.11290765872598989No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10520.11163426407870428No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10260.10887524234291882No Hit
GGCCGAAGGTGATCCCTGGAGTCCAGGTGCTACGCTGCTGACAGTAATAA10090.10707126659259755No Hit
CCCTGTCTTTGTCTGCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGT10060.10675291793077614No Hit
CTCCAGACACCCTGTCTTTGTCTGCAGGGGAGAGAGCCACCCTCTCCTGC10030.10643456926895474No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9980.10590398816591909No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9980.10590398816591909No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT9770.10367554753316927No Hit
CAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAA9770.10367554753316927No Hit
GGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAACGGTCAGAATGGTA9760.10356943131256216No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9760.10356943131256216No Hit
ACCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCACC9710.10303885020952648No Hit
CCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTCTC9710.10303885020952648No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9700.10293273398891935No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGAGA9550.10134099067981234No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9510.1009165257973838No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAACC9490.10070429335616955No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9450.10027982847374102No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACCG200.00666020453.07149554
GACGTTA250.007035271352.3403269
CTAACGC200.00785799850.897613
GGTATCA10300.039.561511
GTATCAA26450.036.845551
ACGGGCT906.0827006E-933.94254716
CCTATAT400.002812681533.9407422
TATCAAC30350.031.98372
ATCAACG30900.031.2962673
CTAATGG850.004270658431.1635770
TCAACGC30950.031.1377244
CAACGCA31250.030.83885
AACGCAG31800.030.6255556
ACGCAGA33750.028.9566237
CGCAGAG34050.028.8011558
GAGTTTA2950.028.7770881
ACGGGAT1203.1941454E-928.28545816
CTATATA605.9746735E-428.2764513
GCAGAGT34650.028.2045029
AGAGTAC34350.028.15446711
Produced by FastQC (version 0.11.5)