FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134633
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44230.3898176767289511No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43000.37897716706635537No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32110.2829989961511784No Hit
GAGTAGTAGTTCAGGGTGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT31560.27815161378172504No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28260.24906731956500472No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27310.24069456819958523No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26770.235935320055031No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25880.22809137403900648No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24540.21628138790252002No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23710.20896624723589036No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20630.1818209059669514No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20610.18164463751715312No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20160.17767859739669126No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20080.17697352359749804No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19070.16807196688268367No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18440.16251951071403703No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17550.15467556469801247No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17280.15229594062573537No Hit
TCCCAGTCCCTCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGG17000.1498281823285591No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17000.1498281823285591No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16980.14965191387876078No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16630.14656721600729047No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16290.14357065236071928No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16270.14339438391092096No Hit
ATATTACTGTCATCAGAGTAGTAGTTCAGGGTGGACGTTCGGCCAAGGGA16070.14163169941293793No Hit
GATTTCACCCTCACCATCAATAGCCTGGAAGTTGAAGATGCTGGAACATA15790.13916394111576166No Hit
CTACTACTCTGATGACAGTAATATGTTCCAGCATCTTCAACTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTC15660.13801819619207267No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15240.1343165587463083No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15090.132994545372821No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15000.1322013373487286No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14900.1313199950997371No Hit
CCATTACCTGCCGGGCCAGTGAGAGCATTGGTGATAGTTTACACTGGTAC14290.12594380738088878No Hit
AGTTTACACTGGTACCAGCAGAAACCGAATCAGTCTCCAAAGCTCCTCAT14250.12559127048129218No Hit
CAGTAATATGTTCCAGCATCTTCAACTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTCCCAGATCCACTGCCA14120.12444552555760321No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13970.12312351218411591No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT13940.12285910950941847No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT13730.12100829078653627No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13570.11959814318814983No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13390.11801172713996509No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13380.11792359291506592No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13280.11704225066607442No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13040.11492702926849474No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTACTTTAGAAGGTGTCCAGTGTGAGGCGCAACTGGTGGAGT12870.11342874744520917No Hit
CTCCTGTGCTGCCTCCGGATTCAGTTTCAGAAATTATGAAATGAGGTGGC12690.1118423313970244No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12540.11052031802353712No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12500.11016778112394052No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12300.10840509662595747No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC12270.10814069395126001No Hit
TAGTTTACACTGGTACCAGCAGAAACCGAATCAGTCTCCAAAGCTCCTCA12210.1076118886018651No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12100.10664241212797441No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTC12090.10655427790307527No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12080.10646614367817611No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT12010.10584920410388204No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11990.10567293565408374No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11940.10523226452958798No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11860.10452719073039476No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11790.1039102511561007No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11740.10346958003160493No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11700.10311704313200833No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC11680.10294077468221002No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11550.10179502975852103No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11440.10082555328463036No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11420.10064928483483206No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11350.10003234526053799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCT2700.049.08434329
CGTATGC2700.047.3695741
ACTAGCG2800.047.2602727
TCGTATG2600.046.4468140
ATGCCGT2750.045.4866644
CTAGCGC2950.044.88693628
GGTATCA13350.044.1440281
GTCGTAT405.0496135E-543.05571439
GCCGTCT2850.042.83416746
ATCTCGT2250.042.6959237
GCGCTCA3250.041.9197531
CGCTCAT3050.041.3569432
ACGAGAC3200.041.2925622
GACTAGC3250.040.69497326
CGAGACT3250.040.6590823
CCGTCTT2950.040.26744547
CACGAGA3300.040.039521
TGTCGTA350.001373833239.2754338
CATCTCG2500.038.35952836
GTATCAA33950.038.2087631
Produced by FastQC (version 0.11.5)