FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134633
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42400.3736891135724062No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35450.3124358272674953No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34970.308205384472336No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32190.2837040699503716No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30010.2644908089223564No Hit
GAGTAGTAGTTCAGGGTGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT29610.2609654399263903No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26760.23584718583013184No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24730.21795593817560394No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23540.2074679654126048No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22480.19812573757329463No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20830.1835835904649345No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20440.18014635569386755No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19380.17080412785455737No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19340.17045159095496076No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19220.16939398025617095No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18680.16463473211161672No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17590.1550281015976091No Hit
CTCCTGTGCTGCCTCCGGATTCAGTTTCAGAAATTATGAAATGAGGTGGC17250.1520315379510379No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17020.15000445077835742No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16920.1491231085293659No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16640.14665535023218962No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16180.1426011758868286No Hit
GATTTCACCCTCACCATCAATAGCCTGGAAGTTGAAGATGCTGGAACATA15910.14022155181455148No Hit
ATATTACTGTCATCAGAGTAGTAGTTCAGGGTGGACGTTCGGCCAAGGGA15720.1385470015414676No Hit
TCCCAGTCCCTCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGG15450.13616737746919047No Hit
CTACTACTCTGATGACAGTAATATGTTCCAGCATCTTCAACTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTC15340.1351979009952998No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15030.1324657400234261No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14910.13140812932463625No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14450.12735395497927524No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14270.1257675389310905No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14230.1254150020314939No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14150.12470992823230065No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14010.12347604908371254No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13920.12268284105962017No Hit
CAGTAATATGTTCCAGCATCTTCAACTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTCCCAGATCCACTGCCA13890.12241843838492271No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13680.12056761966204049No Hit
AGTTTACACTGGTACCAGCAGAAACCGAATCAGTCTCCAAAGCTCCTCAT13590.11977441163794814No Hit
CCATTACCTGCCGGGCCAGTGAGAGCATTGGTGATAGTTTACACTGGTAC13200.11633717686688118No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13170.11607277419218372No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTACTTTAGAAGGTGTCCAGTGTGAGGCGCAACTGGTGGAGT12900.1136931501199066No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12510.11025591534883966No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12340.10875763352555408No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12240.10787629127656255No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTC12190.10743562015206679No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12060.10628987522837781No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC11990.10567293565408374No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11870.10461532495529391No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11770.1037339827063024No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11750.10355771425650409No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11600.1022357008830168No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11590.10214756665811765No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11480.10117809018422698No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG209.378126E-567.666142
CTAACGC252.831322E-454.1162073
GGTATCA15300.037.1647831
GTGGTAT5700.035.0340961
GTATCAA35150.034.761271
ATCGGCG552.4560184E-432.90920361
TGGTATC6200.031.6502912
TATCAAC40650.029.9628682
GTGATCG604.3307492E-429.87502558
AATGGTA2851.8189894E-1229.85940770
ATCAACG42150.028.96793
TCAACGC42100.028.9194134
AACGCAG42350.028.6675766
CAACGCA42850.028.4909115
CGAGCTT1155.748606E-428.4613170
GTATAAC606.063984E-428.2054161
TCGGCGA656.4281706E-427.92529362
GACAGGT2200.027.692591
CGCAGAG44650.027.1908598
GCAGAGT45750.026.3892529
Produced by FastQC (version 0.11.5)