FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657572
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60980.36788748844695734No Hit
GTAGTAGACAGCCGTGTCTTCAGATCTCAGACTCCTCAACTCCATGTAGACTGTGCTCGCGAATCTGTCCCTGGT56540.3411013216922101No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC56270.3394724331733403No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA45670.27552347650660125No Hit
GTATGGTAGCTCACCTAGGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAACGAACTGTGGCTGCACCAT45490.2744375508273547No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43290.2611651258587862No Hit
GTGTATTTCTGTCATCAGTATGGTAGCTCACCTAGGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAACGAA40120.2420407680631671No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38580.23275007058516914No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA38120.2299749271826503No Hit
GTCTACTACTGTGCGGGGGAGGCGGGGCGGGGAAACTGGGTCGCTGACTC37220.22454529878641774No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC36210.21845204914175673No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCCGAAGATT35390.21350505438074485No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35090.21169517824866732No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTATCAGCGACCAGTTGGCCTGGTACC34170.20614489144362957No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32320.19498398862915156No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG32260.19462201340273605No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG31220.1883477761448673No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30800.18581394955995878No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30470.1838230858146735No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTATCAG30020.18110827161655724No Hit
CACCTAGGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC29680.17905707866686937No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29210.17622160605994794No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28780.1736274502706368No Hit
GTATTTCTGTCATCAGTATGGTAGCTCACCTAGGACGTTCGGCCAAGGGA28380.17121428209453346No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27590.1664482749467293No Hit
GTCTGGGGCTGAGGTGAAGAAGACTGGGGCCTCAGTGAGGGTTTCCTGCG27580.16638794574232674No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGGT27310.16475905722345696No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27200.16409543597502854No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA27020.16300951029578203No Hit
CCATAGATGAGGAGCCTGGGAGGCTGGCCATGTTTCTGCTGGTACCAGGCCAACTGGTCGCTGATAACACTCTGA26730.16125996336810708No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG26710.16113930495930193No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCCGAAGATTTCGCAGTGTATTTCTGTCATCAGTATGGTAGCTCA26700.16107897575489935No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATTTCCACCTTGGTCC26650.16077732973288641No Hit
GAAATACACTGCGAAATCTTCGGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA25490.15377914202218665No Hit
TCCTAGGTGAGCTACCATACTGATGACAGAAATACACTGCGAAATCTTCG25370.15305519156935565No Hit
CTACCATACTGATGACAGAAATACACTGCGAAATCTTCGGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC25030.15100399861966782No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24620.14853050123916187No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23610.14243725159450085No Hit
GTCTACATGGAGTTGAGGAGTCTGAGATCTGAAGACACGGCTGTCTACTA23240.14020507103160526No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23050.13905881614795615No Hit
GTCAGAGTGTTATCAGCGACCAGTTGGCCTGGTACCAGCAGAAACATGGC22340.13477544263537272No Hit
GCATTGGGTGCGCCAGGCCCCCGGACAAGGACTTGAGTGGATGGGGTGGA22290.1344737966133598No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC22040.1329655665032952No Hit
GTGCAGCCACAGTTCGTTTCATTTCCACCTTGGTCCCTTGGCCGAACGTC21730.13109536116681508No Hit
GTGTTATCAGCGACCAGTTGGCCTGGTACCAGCAGAAACATGGCCAGCCTCCCAGGCTCCTCATCTATGGTGCAT21410.1291648266259324No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21180.12777725492467296No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20590.1242178318649205No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20460.1234335522076869No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT20280.12234762652844039No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG20270.12228729732403781No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20260.12222696811963521No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT20150.12156334687120682No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC20120.12138235925799905No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19880.11993445835233703No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT19840.11969314153472671No Hit
CAGAAATACACTGCGAAATCTTCGGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA19840.11969314153472671No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCA19830.1196328123303241No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19770.11927083710390862No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC19500.11764194858503883No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19350.11673701051900008No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC19320.11655602290579231No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC19300.11643536449698717No Hit
GAGTGTTATCAGCGACCAGTTGGCCTGGTACCAGCAGAAACATGGCCAGC18780.11329824586805279No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18650.11251396621081919No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18140.10943717678628742No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18130.10937684758188483No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17890.1079289466762228No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT17770.1072049962233918No Hit
ACCATACTGATGACAGAAATACACTGCGAAATCTTCGGGCTCCAGTCTGC17700.10678269179257371No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17640.1064207165661582No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC17600.10617939974854787No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17430.10515380327370395No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17400.10497281566049621No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17310.10442985282087293No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17180.10364557316363936No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17120.10328359793722384No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17100.10316293952841868No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17040.10280096430200317No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16920.10207701384917216No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAT250.001398344878.6907270
CGACGCA150.002475355368.143395
CGTATCG150.002475651468.1413313
ACCGTAT200.007733125751.10599511
GGTATCA14150.047.68781
CGCTAAT3400.044.3012732
TCGCTAA3550.042.39083531
TCGTATG3500.042.36433440
GTCACCG13100.041.0473270
ATCTCGT3200.040.8159937
ACGAGAC3750.039.98951722
CGTATGC3800.039.9833741
ATGCCGT3750.039.77564244
CTTATAC3950.039.6880071
ACTCGCT3900.039.41694629
TATACAC4150.039.4082223
GCCGTCT3800.039.37199446
AATCTCG3400.038.37767436
CGAGACA4000.038.3491723
CACTCGC3950.038.038228
Produced by FastQC (version 0.11.5)