FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657572
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT61380.3703006566230607No Hit
GTAGTAGACAGCCGTGTCTTCAGATCTCAGACTCCTCAACTCCATGTAGACTGTGCTCGCGAATCTGTCCCTGGT56160.33880881192491186No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46850.2826423226261061No Hit
GTATGGTAGCTCACCTAGGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAACGAACTGTGGCTGCACCAT45460.27425656321414693No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44070.2658708038021878No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43520.2625526975600457No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA42500.2563991187109821No Hit
GTGTATTTCTGTCATCAGTATGGTAGCTCACCTAGGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAACGAA41080.24783237168581515No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35390.21350505438074485No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35260.21272077472351125No Hit
GTCTACTACTGTGCGGGGGAGGCGGGGCGGGGAAACTGGGTCGCTGACTC35110.21181583665747247No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCCGAAGATT34920.2106695817738234No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34700.20934233927696655No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA33530.2022838223618642No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32800.1978797904404756No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31830.19202785761342495No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTATCAGCGACCAGTTGGCCTGGTACC31720.1913642363649965No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTATCAG29520.17809181139642805No Hit
GTATTTCTGTCATCAGTATGGTAGCTCACCTAGGACGTTCGGCCAAGGGA28640.17278284140900063No Hit
GTCTGGGGCTGAGGTGAAGAAGACTGGGGCCTCAGTGAGGGTTTCCTGCG28350.17103329448132568No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28290.1706713192549102No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27940.16855979710081975No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27870.16813749267000166No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG27720.1672325546039629No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA27110.16355247313540527No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26750.16138062177691226No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGGT26000.15685593144671844No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25910.1563129686070952No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25700.15504605531464094No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25300.15263288713853757No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCCGAAGATTTCGCAGTGTATTTCTGTCATCAGTATGGTAGCTCA25050.15112465702847297No Hit
TCCTAGGTGAGCTACCATACTGATGACAGAAATACACTGCGAAATCTTCG24850.14991807294042128No Hit
GAAATACACTGCGAAATCTTCGGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA24770.14943543930520062No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATTTCCACCTTGGTCC24560.14816852601274635No Hit
CACCTAGGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC24450.14750490476431793No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24440.14744457555991536No Hit
CCATAGATGAGGAGCCTGGGAGGCTGGCCATGTTTCTGCTGGTACCAGGCCAACTGGTCGCTGATAACACTCTGA23930.14436778613538356No Hit
CTACCATACTGATGACAGAAATACACTGCGAAATCTTCGGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC23630.14255791000330603No Hit
GTGTTATCAGCGACCAGTTGGCCTGGTACCAGCAGAAACATGGCCAGCCTCCCAGGCTCCTCATCTATGGTGCAT23460.14153231352846213No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21980.13260359127687968No Hit
GTGCAGCCACAGTTCGTTTCATTTCCACCTTGGTCCCTTGGCCGAACGTC21950.13242260366367192No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21510.12976811866995822No Hit
GTCAGAGTGTTATCAGCGACCAGTTGGCCTGGTACCAGCAGAAACATGGC21460.1294664726479453No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC21390.12904416821712722No Hit
GTCTACATGGAGTTGAGGAGTCTGAGATCTGAAGACACGGCTGTCTACTA21200.12789791333347814No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20880.12596737879259542No Hit
GAGTGTTATCAGCGACCAGTTGGCCTGGTACCAGCAGAAACATGGCCAGC20120.12138235925799905No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20010.12071873800957061No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19960.1204170919875577No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC19480.11752129017623368No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCA19450.11734030256302591No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19450.11734030256302591No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19330.11661635211019492No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC19180.11571141404415615No Hit
GCATTGGGTGCGCCAGGCCCCCGGACAAGGACTTGAGTGGATGGGGTGGA19160.11559075563535098No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT19160.11559075563535098No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18710.1128759414372347No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC18700.11281561223283212No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18430.11118672371396236No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18420.11112639450955976No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17670.10660170417936597No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC17630.10636038736175563No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17560.10593808293093755No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC17500.10557610770452204No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17380.10485215725169103No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17340.1046108404340807No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17220.10388688998124967No Hit
CAGAAATACACTGCGAAATCTTCGGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17070.10298195191521092No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17060.10292162271080833No Hit
ACCATACTGATGACAGAAATACACTGCGAAATCTTCGGGCTCCAGTCTGC16850.10165470941835408No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16810.10141339260074374No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16720.10087042976112048No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)