FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1140510
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAGGTTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT48150.42217955125338663No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43710.3832495988636663No Hit
ACCTAGGAGACTGTCCTGGTTTTTGCTGGTACCAGGCTAAGTAGTTCCTA36280.31810330466194947No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34720.30442521328177746No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32840.2879413595672111No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30820.27022998483134736No Hit
GTTTTATACAATAGGAACTACTTAGCCTGGTACCAGCAAAAACCAGGACA30150.26435541994370937No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26560.23287827375472378No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25470.22332114580319332No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25310.22191826463599618No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25300.22183058456304636No Hit
ATATTATGGCACTCCTCTTGCGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT22600.19815696486659476No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22350.19596496304284924No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22330.19578960289694963No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21420.18781071625851592No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20800.18237455173562703No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20460.17939342925533314No Hit
GTAATACATCGCCGTGTCCTCAGTTGTCAGACTGTCAATTTGTAGAAAGA20270.17772750786928654No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19990.17527246582669156No Hit
CCTCTTGCGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG19850.17404494480539406No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC19550.17141454261689948No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19320.16939790093905358No Hit
GTCTCCTAGGTTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGT19110.16755661940710737No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC19000.16659213860465932No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18370.1610682940088206No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC18150.15913933240392455No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18070.158437891820326No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGCA17580.1541415682457848No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17410.15265100700563783No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17340.15203724649498906No Hit
GATTTCACTCTCACCATCAGGAACCTGCAGGCTGAAGATGTGGGCGTTTATTACTGTCAGCAATATTATGGCACT17290.15159884613023997No Hit
ATGTATTACTGTGCGAAAGAGGCCCCTGGGACCTACTACGTGGGGTATTATTTTGACTCCTGGGGCCAGGGAACC16910.1482670033581468No Hit
CTCCAGGCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCAGAACCAT16890.14809164321224716No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16530.1449351605860536No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG15750.1380961148959676No Hit
AACCAGGACAGTCTCCTAGGTTGCTCATTTACTGGGCATCTACCCGGGAA15470.13564107285337262No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT15370.1347642721238744No Hit
GTATTACTGTGCGAAAGAGGCCCCTGGGACCTACTACGTGGGGTATTATTTTGACTCCTGGGGCCAGGGAACC14320.12555786446414322No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14160.1241549832969461No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14150.12406730322399627No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14070.12336586264039771No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14000.12275210212974896No Hit
CAGTAATACATCGCCGTGTCCTCAGTTGTCAGACTGTCAATTTGTAGAAA13960.12240138183794969No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13840.12134922096255184No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13620.11942025935765578No Hit
CTGTGGCTCTGGGCGAGACGGCCATCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAATAGGAACTACTTAG13390.1174036176798099No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13370.11722825753391027No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13160.11538697600196404No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGGAACC13110.11494857563721494No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGATGGCCGTCTCGCCCAGAGCCACAGTCAGGGAATCTGGAGA13050.11442249519951601No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT12980.11380873468886726No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12750.11179209301102137No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12630.11073993213562355No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12620.11065225206267372No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12440.10907401074957694No Hit
CGCAAGAGGAGTGCCATAATATTGCTGACAGTAATAAACGCCCACATCTT12350.10828489009302857No Hit
CAAGAGGAGTGCCATAATATTGCTGACAGTAATAAACGCCCACATCTTCA12200.10696968899878123No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12050.10565448790453393No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11990.10512840746683501No Hit
CTCCTCTTGCGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC11970.10495304732093538No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11940.1046900071020859No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGGTGACCCAGTCTCCAGATTCCCTGACTGTGGCTCTGGGCGAGACGG11930.10460232702913608No Hit
ACTTAGCCTGGTACCAGCAAAAACCAGGACAGTCTCCTAGGTTGCTCATT11870.10407624659143717No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAACCTAGGAGACTGTCCTGGTTTTTGCTGGTA11850.10390088644553752No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11640.1020596049135913No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11590.10162120454884219No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11510.10091976396524362No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)