FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1182264
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC56380.4768816440321282No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGTATGAGAACTAACATCCCACAC41660.35237476570376836No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33940.28707632136307965No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG33720.28521548486632425No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29290.247745004499841No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT27080.229052056055162No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26770.22642996826427939No Hit
GGATTACCTGTGGGGGGAATAACATTGGAAGTAAAAGTGTTCACTGGTACCAGCAGAAGCCAGGCCAGGCCC25720.2175487031661287No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC25060.21196619367586256No Hit
GTAATATACGCCCATGTCCGCAGCGGTCACAGAGCTCAACCTCAGGGAGA23700.20046284078682935No Hit
CCCCTGTGCTGGTCGTATATGATGATAGCGACCGGCCCTCAGGGATCCCT23390.19784075299594675No Hit
GTCTCTGGTGGCTCCATCAGTACTTCCTACTGGAGCTGGATCCGGCAGCC21760.1840536462245319No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21740.18388447927028143No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG21500.18185447581927555No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21460.18151614191077456No Hit
CCCCAGGACAGACGGCCAGGATTACCTGTGGGGGGAATAACATTGGAAGT21320.18033197323102115No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC19630.16603736559685486No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19560.16544528125697816No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19480.16476861343997617No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG19390.164007362145849No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19300.16324611085172178No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT19210.1624848595575946No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19060.1612161074007159No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18990.1606240230608392No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG18930.16011652219808772No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18780.1588477700412091No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17980.1520810918711895No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGAACACTTTTACTT17240.1458219145639214No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17100.144637745884168No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17090.14455316240704277No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17070.1443839954527923No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16400.1387169024854009No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16240.137363566851397No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT16230.13727898337427175No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGGTCCAACTCTGGGAACACGG15950.1349106460147649No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15900.13448772862913866No Hit
GTATGAGAACTAACATCCCACACCTGACAGTAATAGTCGGCCTCATCCCC15570.1316964738840056No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15390.1301739712957512No Hit
GTATATTACTGTGCGAGACTCCGGGCCGGGTATAGCAACAGCTGGTACCC15320.1295818869558745No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15140.1280593843676201No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14950.12645229830224045No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14940.1263677148251152No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14470.12239229140022871No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14300.12095437228909955No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGTACTTCCTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGA13820.11689436538708782No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTAC13720.11604853061583538No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC13710.11596394713871012No Hit
CTATTACTGTCAGGTGTGGGATGTTAGTTCTCATACTGTGGTATTCGGCG13690.11579478018445964No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC13520.11435686107333048No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC13460.11384936021057904No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG13410.11342644282495282No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGC13350.11291894196220134No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13310.11258060805370035No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13300.11249602457657512No Hit
GGCCAGGCCCCTGTGCTGGTCGTATATGATGATAGCGACCGGCCCTCAGG13130.11105810546544596No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA13130.11105810546544596No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT13110.11088893851119548No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA13090.11071977155694497No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13020.11012768721706827No Hit
CAGTAATATACGCCCATGTCCGCAGCGGTCACAGAGCTCAACCTCAGGGAGAACTGGTTCTTGGACGTGTCTA12990.10987393678569253No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGTATGAGAA12880.10894351853731483No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12840.10860518462881387No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12780.10809768376606241No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12770.10801310028893715No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12480.10556017945230506No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGTATGAGAAC12370.10462976120392738No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12270.10378392643267494No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT12200.10319184209279823No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12190.10310725861567298No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG12120.10251517427579628No Hit
GAATAACATTGGAAGTAAAAGTGTTCACTGGTACCAGCAGAAGCCAGGCC11990.1014155890731681No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11960.10116183864179236No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)