FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1182264
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC50950.4309528159531204No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGTATGAGAACTAACATCCCACAC37960.3210788791674279No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG30990.26212419561113254No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30190.25535751744111296No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30100.2545962661469858No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26420.22346954656489582No Hit
GGATTACCTGTGGGGGGAATAACATTGGAAGTAAAAGTGTTCACTGGTACCAGCAGAAGCCAGGCCAGGCCC26230.2218624604995162No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25560.21619536753212482No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24870.21035910761048293No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22560.19082032439455146No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC22230.1880290696494184No Hit
CCCCTGTGCTGGTCGTATATGATGATAGCGACCGGCCCTCAGGGATCCCT22110.18701406792391548No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22060.18659115053828926No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG22000.18608364967553778No Hit
GTCTCTGGTGGCTCCATCAGTACTTCCTACTGGAGCTGGATCCGGCAGCC20790.1758490489433832No Hit
GTAATATACGCCCATGTCCGCAGCGGTCACAGAGCTCAACCTCAGGGAGA20540.17373446201525208No Hit
CCCCAGGACAGACGGCCAGGATTACCTGTGGGGGGAATAACATTGGAAGT19080.16138527435496638No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG18960.16037027262946346No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG18360.1552952640019488No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18120.15326526055094292No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17870.15115067362281184No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17580.14869775278617975No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17410.14725983367505058No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16740.1415927407076592No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16700.1412544067991582No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT16530.13981648768802907No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16460.13922440334815234No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGAACACTTTTACTT16420.13888606943965137No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16370.13846315205402515No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC15990.13524897992326587No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15950.1349106460147649No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15730.13304980951800952No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15670.13254230865525804No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT15660.13245772517813278No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15420.13042772172712694No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15370.13000480434150072No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15280.12924355304737353No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15120.12789021741336962No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14980.12670604873361618No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGGTCCAACTCTGGGAACACGG14910.12611396439373945No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGC14530.12289979226298019No Hit
GGCCAGGCCCCTGTGCTGGTCGTATATGATGATAGCGACCGGCCCTCAGG14510.12273062530872969No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14460.12230770792310347No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTAC14250.12053145490347333No Hit
GTATGAGAACTAACATCCCACACCTGACAGTAATAGTCGGCCTCATCCCC14130.11951645317797041No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14010.11850145145246747No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13870.11731728277271404No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13760.11638686452433634No Hit
CTATTACTGTCAGGTGTGGGATGTTAGTTCTCATACTGTGGTATTCGGCG13750.11630228104721112No Hit
GTATATTACTGTGCGAGACTCCGGGCCGGGTATAGCAACAGCTGGTACCC13750.11630228104721112No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13700.1158793636615849No Hit
GTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG13590.1149489454132072No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13570.11477977845895672No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA13490.11410311064195477No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13300.11249602457657512No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGTACTTCCTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGA13130.11105810546544596No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12840.10860518462881387No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG12690.1073364324719352No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12590.10649059770068275No Hit
GGGTATATCTATTATAATGGGAACACCAACTACAACTCCTCCCTCAAGAG12550.10615226379218179No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12380.10471434468105262No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12380.10471434468105262No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC12220.10336100904704872No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC12160.10285350818429724No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC12130.1025997577529215No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA12070.10209225689017006No Hit
GTCCAACTCTGGGAACACGGCCACGCTGACCATCAGCAGGGTCGAAGCCG12060.1020076734130448No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11890.10056975430191564No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11850.10023142039341468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC3750.043.945972
GGTATCA11350.041.1527751
GTATCAA30400.039.880191
ATCAACG35200.034.1319433
TATCAAC35400.034.0418362
TCAACGC35600.033.650594
CAACGCA36500.033.0075385
AACGCAG37000.032.561496
ACGCAGA39050.030.6763217
CGCAGAG39250.030.4325588
TACTAGT604.466432E-429.71827366
AGAGTAC40650.029.4688911
CAGAGTA41150.029.19423510
GCAGAGT41200.028.992189
TGGATTG14000.028.71123970
GAGTTTA4800.027.9048371
ATACGTA1005.247184E-727.4592611
CCTGCGA500.00793037827.459268
AGTTTAT5050.025.8345742
GTGGTGA3950.025.02328370
Produced by FastQC (version 0.11.5)