FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1260143
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCAGCAGTATGATACCTCACCGACCACTTTTGGCCAGGGGA71180.564856528187674No Hit
GTATGATACCTCACCGACCACTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCAT63010.500022616480828No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52220.4143974136268661No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48380.38392468156391774No Hit
GTATCATACTGCTGACAATAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC40230.31924948200323294No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36720.29139550035194417No Hit
CAATAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA36060.2861579995286249No Hit
GTAATATACAGCCGTGTCGTCGCCTCTGAGACTATTCATGTGCAAGTACA35790.28401538555544886No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCA33060.2623511776044465No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31420.24933678161922893No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31030.24624189476908573No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30900.24521026581903801No Hit
GTCTTTGTCTGCGGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA30850.24481348545363504No Hit
CTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCA29680.23552882490320542No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29240.23203715768765923No Hit
GTATATTACTGTGCAAGAGGGCCGTATAGTACCTCGTCCGAAATAGGCTT29010.23021196800680557No Hit
CTCCTGCAGGGCCAGTCAGAATATTGACAGCAAATACTTGAGCTGGTACC29000.230132611933725No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28270.22433961859884155No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28110.22306992142955206No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26230.2081509796904002No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26110.20719870681343305No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGATT25910.20561158535182117No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24190.19196234078195887No Hit
CTCCAGGCACTCTGTCTTTGTCTGCGGGGGAAAGAGCCACCCTCTCCTGC22380.1775988915543712No Hit
CATCTATGGTGCATCCACCAGGGCCTCCGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA22370.1775195354812906No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21920.17394851219266386No Hit
GTCTCCAGGCACTCTGTCTTTGTCTGCGGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAATATTGACAG21760.17267881502337434No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21420.1699807085386341No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20730.16450513949607307No Hit
GGCCAAAAGTGGTCGGTGAGGTATCATACTGCTGACAATAATACACTGCA20550.16307673018062235No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCGGAGGCCCTGGT19820.15728373684573893No Hit
GTATAGTACCTCGTCCGAAATAGGCTTGACCAACTACCAGTACTACTACA19050.15117331921853314No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19010.15085589492621077No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18830.14942748561076005No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18490.14672937912601983No Hit
GAATATTGACAGCAAATACTTGAGCTGGTACCAGCAGAAATCTGGCCGGG18110.14371384834895723No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17970.1426028633258289No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGATTTTGCAGTGTATTATTGTCAGCAGTATGATACCTCA17920.14220608296042592No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17580.1395079764756857No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17250.13688922606402606No Hit
GTGTATTATTGTCAGCAGTATGATACCTCACCGACCACTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAA17070.13546081674857535No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17020.13506403638317238No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16660.13220721775227098No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCCCCTGGCCAAAAGTG16620.1318897934599486No Hit
CCATAGATGAGGAACCTGGGAGCCCGGCCAGATTTCTGCTGGTACCAGCTCAAGTATTTGCTGTCAATATTCTGA16390.1300646037790949No Hit
GTACTATACGGCCCTCTTGCACAGTAATATACAGCCGTGTCGTCGCCTCT16200.12855683839056362No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16030.1272077851481935No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCC15970.12673164870970993No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAAC15700.12458903473653386No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15630.12403354222496969No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15420.12236706469027721No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15130.12006573857093997No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT14990.11895475354781163No Hit
ATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCAC14800.11744698815928034No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14640.11617729098999081No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14570.11562179847842664No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14530.11530437418610427No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14070.11165399482439692No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14040.11141592660515512No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCG14010.11117785838591335No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14000.11109850231283275No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13690.10863846404733431No Hit
TCTATGGTGCATCCACCAGGGCCTCCGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT13680.10855910797425371No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCACCGGAGAAATTGTGTTGACGCAGTCTC13500.10713069865880302No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13410.10641649400107765No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG13370.10609906970875528No Hit
CTGCTACTCTGGCTCCCAGAAACCACCGGAGAAATTGTGTTGACGCAGTC13260.10522615290486872No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13170.10451194824714338No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13060.10363903144325684No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13020.10332160715093447No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12790.10149641747008079No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT350.006191957754.05726270
CGTATGC2100.051.03349741
ATCTCGT2150.047.98342537
TCGTATG2400.047.45108440
GTACGTA551.6435642E-646.83796369
ATGCCGT2300.046.79763444
ACGAGAC2900.042.2617122
GTATGCC2650.041.8018142
GCCGTCT2500.041.79696346
CCCACGA2950.041.53552219
CTTATAC3150.038.9045221
ATCTCCG3150.038.90297710
TAAGACA3200.038.47683331
TATATCG451.08422515E-437.820845
TATACAC3450.037.491963
GTATTAC558.105855E-637.136131
TCTGGAG11550.037.13023870
CGCTCGG851.2140274E-437.09811870
GTATCAA38500.036.6940351
TCTCCGA3350.036.58186311
Produced by FastQC (version 0.11.5)