FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1260143
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCAGCAGTATGATACCTCACCGACCACTTTTGGCCAGGGGA70640.5605713002413218No Hit
GTATGATACCTCACCGACCACTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCAT61800.49042053163807603No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52230.41447676969994673No Hit
GTATCATACTGCTGACAATAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC43580.34583376648523223No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39320.31202807935289884No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36780.2918716367904277No Hit
CAATAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA34490.2736990960549715No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34330.272429398885682No Hit
GTAATATACAGCCGTGTCGTCGCCTCTGAGACTATTCATGTGCAAGTACA33050.26227182153136586No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31930.2533839413463393No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCA31450.2495748498384707No Hit
GTCTTTGTCTGCGGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA30350.24084568179960525No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29560.23457655202623828No Hit
CTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCA29350.2329100744915458No Hit
CTCCTGCAGGGCCAGTCAGAATATTGACAGCAAATACTTGAGCTGGTACC28180.22362541394111618No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27770.22037181494481184No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27620.2191814738486029No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGATT27450.21783242060623279No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25850.20513544891333763No Hit
GTATATTACTGTGCAAGAGGGCCGTATAGTACCTCGTCCGAAATAGGCTT24760.1964856369475528No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24110.19132749219731413No Hit
CTCCAGGCACTCTGTCTTTGTCTGCGGGGGAAAGAGCCACCCTCTCCTGC23230.18434415776622176No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22980.1823602559392069No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22910.18180476342764273No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22250.17656726260432348No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22060.17505949721579217No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21110.16752067027313566No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20920.16601290488460438No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20390.16180703301133284No Hit
GTCTCCAGGCACTCTGTCTTTGTCTGCGGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAATATTGACAG20240.1606166919151239No Hit
CATCTATGGTGCATCCACCAGGGCCTCCGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA20140.15982313118431796No Hit
GAATATTGACAGCAAATACTTGAGCTGGTACCAGCAGAAATCTGGCCGGG19520.1549030546533211No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19500.1547443425071599No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19410.15403013784943453No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCGGAGGCCCTGGT19320.1533159331917092No Hit
GGCCAAAAGTGGTCGGTGAGGTATCATACTGCTGACAATAATACACTGCA18670.14815778844147054No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18650.14799907629530934No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGATTTTGCAGTGTATTATTGTCAGCAGTATGATACCTCA17720.14061896149881403No Hit
GTGTATTATTGTCAGCAGTATGATACCTCACCGACCACTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAA16980.13474661209085No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCCCCTGGCCAAAAGTG16400.1301439598521755No Hit
GTACTATACGGCCCTCTTGCACAGTAATATACAGCCGTGTCGTCGCCTCT15820.12554130761350102No Hit
GTATAGTACCTCGTCCGAAATAGGCTTGACCAACTACCAGTACTACTACA15610.12387483007880852No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15390.12212899647103544No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15290.12133543574022948No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15150.12022445071710114No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15120.11998638249785937No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT14970.11879604140165045No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAAC14710.11673278350155498No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14670.1164153592092326No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14610.11593922277074904No Hit
CTGCTACTCTGGCTCCCAGAAACCACCGGAGAAATTGTGTTGACGCAGTC14600.11585986669766844No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCG14300.11347918450525059No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14210.11276497984752523No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13820.10967009299738205No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13730.10895588833965669No Hit
ATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCAC13430.10657520614723885No Hit
CCATAGATGAGGAACCTGGGAGCCCGGCCAGATTTCTGCTGGTACCAGCTCAAGTATTTGCTGTCAATATTCTGA13400.10633713792799705No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13210.10482937253946575No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13210.10482937253946575No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13200.10475001646638515No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12990.10308353893169267No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12960.1028454707124509No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCC12940.1026867585662897No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12860.10205190998164496No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT400.00905680449.09980870
GTGGTAT4700.039.7652051
TCTGGAG11500.038.14130470
TGGTATC5000.036.6880152
GTACGTA601.4073108E-436.1801569
GTATCAA42500.034.8606871
AACTGTG33600.031.95384470
TATCAAC47000.031.3683952
TCAACGC48950.030.3125424
ATCAACG48650.030.2924863
ACACCGT8450.030.19596348
CAACGCA49700.029.9917435
AACGCAG49950.029.842826
CGTGTTC8500.029.37712552
CGCAGAG51700.028.700178
ACGCAGA52250.028.594157
TACGGGC5400.028.30303615
ACCGTGT8850.028.12975750
CACCGTG9100.028.08729649
AGAGTAC52350.028.02062811
Produced by FastQC (version 0.11.5)