FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501117
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTCCCTCACCAAAGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA66370.4421374216666656No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46370.30890330334011273No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41670.2775932855333728No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCACTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACGCACTGCCA39840.2654023637064932No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGCCTCAGGGAGA38610.2572084654294102No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG34940.23276000471648778No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA34610.23056164176409968No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31230.20804507576691225No Hit
GTATGGTCCCTCACCAAAGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT29730.1980525168924208No Hit
GTGTATTACTGTCAGCAGTATGGTCCCTCACCAAAGTACACTTTTGGCCA28140.18746040448545984No Hit
CATCTATGCTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGCGTCTGGGACAGACTTCA27980.1863945315388474No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27930.18606144624303103No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26070.17367067323866162No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24990.16647603084902776No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG24900.16587647731655827No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCAGCAGCAGCTACTTAGCCTGGTACC24780.16507707260659893No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA24080.1604138784651696No Hit
GTGTATTACTGTGCGAGAGCTGGGAGCTATTTCCTTGACTACTGGGGCCA23830.1587484519860877No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23560.15694979138867923No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23460.15628362079704647No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22880.15241983136557644No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22410.14928882958490244No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22220.1480231054608002No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22190.14782325428331036No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT21980.14642429604088156No Hit
GTCTCCAGGCACCCTGTCCTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGCAG21720.14469225250263637No Hit
GGACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCACTCTGCTGATGGTGAGAGTGAAGTCTGTC20900.1392296536512477No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20610.1372977589355127No Hit
GTAATACACTGCAAAATCTTCAGGCTCCACTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACGCACTGCCA20240.13483292774647146No Hit
CTCCAGGCACCCTGTCCTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC19760.1316353089066342No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTAACTGGTGGAGTTGGGTCCGCCA19390.12917047771759296No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19310.12863754124428675No Hit
GTCCTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA18760.12497360299030655No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18140.12084334532218341No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC18030.12011055767138737No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC17550.11691293883155011No Hit
GCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTGCTGCTGACACTCTGA17480.11644661941740717No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC17000.1132490005775699No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT16960.1129825323409168No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16760.11165019115765126No Hit
CAGTAATACACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGCCTCAGGGAGAGCTGGTTCCTGGAGTTGTCTA16710.1113171058618349No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACTTTGGTGAGGGACCATACTG16540.11018461585605918No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16260.10831933819948744No Hit
GACTTCACTCTCACCATCAGCAGAGTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTCCCTCA16140.10751993348952814No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC16080.10712023113454848No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15990.10652067760207899No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT15240.10152439816483326No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15210.10132454698734343No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT15180.1011246958098536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGC554.3655746E-1156.1015535
CTAACGC252.7704582E-454.3560033
ATCGCGT453.128298E-853.44789537
TACGCGA200.007170317752.0918644
TACGTAT652.0292597E-550.23715670
CGCGTAT352.2855076E-549.24073839
TATCGCG652.2555469E-1047.51366836
TCGTATG2050.045.48210540
CCTAACG306.8099255E-445.2966732
CGTATGC2250.044.57221241
GGTATCA16550.042.904691
AGTATCG803.6379788E-1142.81936634
GACCGAT3500.040.8268426
ACCGATC3550.040.27627
GTATGCC2550.039.39120542
ACGAGAC3650.039.10727722
TATACAC4450.038.934783
TCGCGTA451.0165615E-438.23761438
CTTATAC4200.037.2104571
GAGACCG3850.037.09303724
Produced by FastQC (version 0.11.5)