FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501117
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTCCCTCACCAAAGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA62620.41715602448043687No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47950.3194287986879104No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42070.28025796789990387No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCACTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACGCACTGCCA37250.24814854538320463No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGCCTCAGGGAGA36210.24122037123022388No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG35040.23342617530812057No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34570.23029517352744655No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33100.22050246583044494No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA31590.21044328989679018No Hit
GTGTATTACTGTCAGCAGTATGGTCCCTCACCAAAGTACACTTTTGGCCA29670.19765281453744113No Hit
GTATGGTCCCTCACCAAAGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT28360.18892597978705192No Hit
CATCTATGCTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGCGTCTGGGACAGACTTCA26420.1760022703093763No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26100.17387052441615145No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25910.1726048002920492No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA25110.16727543555898708No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCAGCAGCAGCTACTTAGCCTGGTACC24450.16287870965421083No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24220.16134651729345548No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23780.15841536669027131No Hit
GTGTATTACTGTGCGAGAGCTGGGAGCTATTTCCTTGACTACTGGGGCCA23520.15668332315202613No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG22180.14775663722414709No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21760.14495872073928948No Hit
GGACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCACTCTGCTGATGGTGAGAGTGAAGTCTGTC21060.14029552659786013No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20910.13929627071041098No Hit
GTAATACACTGCAAAATCTTCAGGCTCCACTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACGCACTGCCA20810.13863010011877822No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC20690.1378306954088189No Hit
GTCTCCAGGCACCCTGTCCTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGCAG20570.13703129069885958No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19760.1316353089066342No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19700.13123560655165453No Hit
GTCCTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA19150.12757166829767433No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19060.12697211476520484No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18380.12244215474210204No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18150.1209099623813467No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18130.12077672826302013No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT17860.11897806766561167No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17830.11877821648812184No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTAACTGGTGGAGTTGGGTCCGCCA17750.11824528001481563No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17550.11691293883155011No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17350.11558059764828457No Hit
CTCCAGGCACCCTGTCCTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC17130.11411502234669249No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACTTTGGTGAGGGACCATACTG16990.11318238351840663No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC16850.11224974469012075No Hit
GCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTGCTGCTGACACTCTGA15810.10532157053714002No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT15750.10492186818216034No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT15410.10265688817060895No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC15390.1025236540522824No Hit
GACTTCACTCTCACCATCAGCAGAGTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTCCCTCA15260.1016576322831598No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC15150.10092484463236377No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15090.10052514227738411No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17200.042.013371
TACTGAT15800.034.27236670
AATGGTA5850.031.24057670
TATCAAC44250.030.5104262
GTATAGA450.0050182830.15671
ATCAACG45350.029.8411923
TCAACGC46250.029.3309044
GTATCAA46950.029.193231
CAACGCA46600.029.1843575
AACGCAG47500.028.702796
CCGTCAT500.007163431528.03797749
TCCGAGA8750.027.84874370
ACGCAGA49400.027.3928747
CGTAGTA500.00840058627.1374132
CGTGAAA1000.00849255927.07516970
CGCAGAG50050.027.0380238
AGAGTAC49700.027.02190611
TAACGGC809.976462E-525.7146436
TCTACTG12850.025.7050868
GTGAGCG6700.025.25668770
Produced by FastQC (version 0.11.5)