FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1399110
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC61220.43756387989507617No Hit
CTTTAGAGGTGTTCGGCGGAGGGACCACGGTGACCGTCCTAGGTCAGCCC52120.3725225321811723No Hit
CCTCTAAAGTGTAGCTGCCTCCATATGAGGAGCAGTAATAATCAGCCTCA50450.3605863727655438No Hit
GATTATTACTGCTCCTCATATGGAGGCAGCTACACTTTAGAGGTGTTCGG50000.35737004238408704No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT46600.3330688795019691No Hit
GCCTTGGGCTGACCTAGGACGGTCACCGTGGTCCCTCCGCCGAACACCTC43650.311984047001308No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG40820.29175690260236864No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG36530.26109455296581396No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT35800.2558769503470063No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCGTGGTCCCTC33520.23958087641429193No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC33070.23636454603283516No Hit
GACCTAGGACGGTCACCGTGGTCCCTCCGCCGAACACCTCTAAAGTGTAG33060.23629307202435837No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG32840.23472064383786836No Hit
GTGTCCGGGTCTCCTGGACAGTCGGTCACCGTCTCCTGCACTGGGACCAG32150.22978893725296795No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG31800.22728734695627936No Hit
CTCCTGCACTGGGACCAGCAGTGATATTGGTGCTTCTAACTATGTCTCCTGGTACCAACAACACCCAGGCACAGC29590.2114915910829027No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28970.20706020255734No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT28860.20627398846409503No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26660.1905497065991952No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTCCTCATAT26590.19004938853985748No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC26320.1881195903109834No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT26260.1876907462601225No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG25980.1856894740227716No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25300.18082924144634804No Hit
CTCCTGGACAGTCGGTCACCGTCTCCTGCACTGGGACCAGCAGTGATATT25080.17925681325985807No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCGGTCAC24850.17761291106489124No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG24470.1748968987427722No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT24220.17311004853085174No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23910.1708943542680704No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCGGTCACCGTCTCCTG23170.16560527764078592No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22920.1638184274288655No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22910.16374695342038867No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG22600.16153125915760733No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG22010.1573142926574751No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20950.14973804775893246No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC20900.14938067771654837No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20700.14795119754701203No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT20600.14723645746224384No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC20230.14459191914860162No Hit
GTAATACATGGCTGTGTCCGCGACAGTCACAGAGGTCAGCTTCAGGGAGA19520.13951726454674757No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG19480.13923136851284032No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGG19050.13615798614833716No Hit
CCCCTGAGGGCCGCTTAATAACATCATAAATTATGAGCTTGGGGGCTGTGCCTGGGTGTTGTTGGTACCAGGAGA18760.13408523990250945No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18710.13372786986012536No Hit
GGATGAGGCTGATTATTACTGCTCCTCATATGGAGGCAGCTACACTTTAG18560.13265575973297308No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18390.13144070158886723No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18340.13108333154648313No Hit
GTCGGTCACCGTCTCCTGCACTGGGACCAGCAGTGATATTGGTGCTTCTAACTATGTCTCCTGGTACCAACAACA18330.13101185753800632No Hit
CCCAGGCACAGCCCCCAAGCTCATAATTTATGATGTTATTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGG17810.12729520909721181No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17550.12543688487681454No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC17480.12493656681747682No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG17160.12264939854621866No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACC16990.12143434040211276No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16800.12007633424105323No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC16600.1186468540715169No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC16510.11800358799522555No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16350.11686000385959647No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT16170.11557347170701375No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCGGTCACCGTCTCCTGCACTGGGACCAGCAGTGATATTGGTG15820.11307188141032513No Hit
ACCTAGGACGGTCACCGTGGTCCCTCCGCCGAACACCTCTAAAGTGTAGC15510.11085618714754379No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCGGTCAC14960.10692511668131885No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCGGTCACCGTCT14950.10685364267284203No Hit
GACCATCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTCCT14760.1054956365117825No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14690.10499531845244478No Hit
GTGTAGCTGCCTCCATATGAGGAGCAGTAATAATCAGCCTCATCCTCAGC14550.10399468233376934No Hit
CCTCATATGGAGGCAGCTACACTTTAGAGGTGTTCGGCGGAGGGACCACGGTGACCGTCCTAGGTCAGCCCAAGG14510.10370878629986206No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14290.10213635811337207No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCGTGGTC14260.10192193608794162No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT14230.10170751406251118No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC14130.10099277397774299No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)