FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1399110
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC56310.4024701417329588No Hit
CCTCTAAAGTGTAGCTGCCTCCATATGAGGAGCAGTAATAATCAGCCTCA51180.36580397538435144No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47730.34114544245984946No Hit
CTTTAGAGGTGTTCGGCGGAGGGACCACGGTGACCGTCCTAGGTCAGCCC47010.33599931384951864No Hit
GATTATTACTGCTCCTCATATGGAGGCAGCTACACTTTAGAGGTGTTCGG44770.31998913595071155No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG43910.3138423712217052No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT42250.30197768581455353No Hit
GCCTTGGGCTGACCTAGGACGGTCACCGTGGTCCCTCCGCCGAACACCTC41490.29654566117031544No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG38700.27660441280528336No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG32990.2357927539650206No Hit
GTGTCCGGGTCTCCTGGACAGTCGGTCACCGTCTCCTGCACTGGGACCAG32840.23472064383786836No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32730.23393442974462336No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT31960.22843093109190843No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCGTGGTCCCTC31950.22835945708343158No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT30830.22035436813402806No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30070.21492234348978995No Hit
GACCTAGGACGGTCACCGTGGTCCCTCCGCCGAACACCTCTAAAGTGTAG29680.21213485715919408No Hit
CTCCTGCACTGGGACCAGCAGTGATATTGGTGCTTCTAACTATGTCTCCTGGTACCAACAACACCCAGGCACAGC29440.21041948095575047No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG29270.20920442281164453No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28180.20141375588767144No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCGGTCAC27290.1950525691332347No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26160.18697600617535434No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTCCTCATAT25850.18476031191257297No Hit
CTCCTGGACAGTCGGTCACCGTCTCCTGCACTGGGACCAGCAGTGATATT25320.18097218946330168No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24140.17253825646303722No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG24000.17153762034436176No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23820.17025108819177906No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA23650.16903603004767317No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC23350.16689180979336865No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22650.16188862919999142No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCGGTCACCGTCTCCTG22230.15888672084396507No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG21900.15652807856423012No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21390.15288290413191244No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC21260.1519537420217138No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC20910.14945215172502518No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20650.14759382750462793No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG20620.14737940547919748No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT20620.14737940547919748No Hit
GTCGGTCACCGTCTCCTGCACTGGGACCAGCAGTGATATTGGTGCTTCTAACTATGTCTCCTGGTACCAACAACA19350.13830220640264168No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18940.13537177205509215No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18380.1313692275803904No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18230.13029711745323813No Hit
GGATGAGGCTGATTATTACTGCTCCTCATATGGAGGCAGCTACACTTTAG17940.12822437120741043No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG17800.127223735088735No Hit
GTAATACATGGCTGTGTCCGCGACAGTCACAGAGGTCAGCTTCAGGGAGA17760.12693783905482772No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17720.12665194302092045No Hit
CCCCTGAGGGCCGCTTAATAACATCATAAATTATGAGCTTGGGGGCTGTGCCTGGGTGTTGTTGGTACCAGGAGA17550.12543688487681454No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17200.12293529458012593No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC17000.12150581441058958No Hit
GTGTAGCTGCCTCCATATGAGGAGCAGTAATAATCAGCCTCATCCTCAGC16870.12057665230039095No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16790.12000486023257641No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGG16780.1199333862240996No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16650.11900422411390099No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG16570.11843243204608644No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC16130.11528757567310648No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15990.11428693955443103No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT15760.11264303735946422No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCGGTCAC15530.11099913516449743No Hit
CCCAGGCACAGCCCCCAAGCTCATAATTTATGATGTTATTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGG15460.1104988171051597No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACC15170.108426070859332No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15120.1080687008169479No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCGGTCACCGTCTCCTGCACTGGGACCAGCAGTGATATTGGTG14960.10692511668131885No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14280.10206488410489525No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCGTGGTC14260.10192193608794162No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14130.10099277397774299No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCGGTCACCGTCT14130.10099277397774299No Hit
ACCTAGGACGGTCACCGTGGTCCCTCCGCCGAACACCTCTAAAGTGTAGC14010.10013508587602118No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGACG200.007601103351.32866330
GTGGTAT3550.046.1026271
GGTATCA13100.043.727111
TGGTATC3950.041.4147572
GTATCAA32000.037.29331
GTACGAC400.00275882134.076023
ATCAACG37250.031.5603393
TATCAAC37600.031.3616732
TCAACGC38200.030.5970544
CAACGCA38850.030.0851335
AACGCAG39350.029.8760536
TGTTAGA6000.028.87012570
ACGCAGA41000.028.6737237
GAGTTTA4050.028.624421
CGCAGAG41450.028.198018
AGAGTAC42200.027.77760711
GCAGAGT42750.027.3405259
CAGAGTA43050.027.22915310
GACAGGT3350.026.4631371
GGTAGAA30450.025.38033170
Produced by FastQC (version 0.11.5)