FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1601427
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT80810.5046124487722512No Hit
GCTTAATAGTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT80300.5014277890906048No Hit
CTATTAAGCTGTTGACAGTAATAAGTCGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC74890.4676454187421593No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC67890.42393440350387496No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC61950.38684248485881656No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC54750.3418825834708669No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC50440.3149690869455804No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT48190.30091911776184616No Hit
GTCTCCATCCTTCCTGTCTGCTTCTGTGGGAGACAGAGTCACCATCACTT46460.2901162525672416No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA43850.27381828831410987No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG43380.2708834058623965No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT42900.26788607910319984No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT41000.2560216606813798No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG39780.24840345516842166No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT38500.24041058381056396No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG37500.2341661530622376No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG37060.23141860353297403No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG36320.22679772477921253No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT36260.22642305893431297No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC35820.22367550940504935No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG34410.21487086204990924No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA34120.2130599771328946No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC34050.21262286698051175No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG33960.21206086821316236No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG33060.20644088053966866No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT32790.2047548842376206No Hit
GGGCATTAACAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAACCTCCTGATCTTTGCTGCATC30450.19014291628653696No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT29830.18627136922257462No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCGACTTATTACTGTCAACAGCTTAATAGTTAC29600.18483515015045957No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29370.1833989310783445No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT29310.18302426523344492No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG29160.18208760062119597No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG28990.18102604739398048No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC27770.17340784188102237No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAACAGTTATTTAGCCTGGTATCAGCAAAAA27660.17272095449870647No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA26710.16678874528779647No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26270.16404119575853285No Hit
ACTTATTACTGTCAACAGCTTAATAGTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA26240.16385386283608308No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG25980.16223031084151823No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA24960.15586099147822535No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT24810.1549243268659764No Hit
GATCAGGAGGTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAAT24110.15055322534214796No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT23900.14924189488499945No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA23840.14886722904009986No Hit
GCCCCTAACCTCCTGATCTTTGCTGCATCCACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGAT23380.14599479089586975No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC23360.1458699022809032No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23130.14443368320878816No Hit
GTGTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTA22660.1414988007570748No Hit
GTAATAAGTCGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG22300.1392508056876773No Hit
GGTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAATAACTGTTA22170.13843902969039487No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC22150.13831414107542836No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT22090.13793947523052877No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC22060.13775214230807897No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT22030.1375648093856292No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA21820.13625347892848066No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT21750.13581636877609782No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC21540.1345050383189493No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTG21520.13438014970398277No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC21390.13356837370670033No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA20950.13082082417743673No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT20890.13044615833253717No Hit
CAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAACCTCC20810.12994660387267107No Hit
ACCTTGATGGGACCCCACTTTGTAAAGTGGATGCAGCAAAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTTTG20780.12975927095022127No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC20110.12557550234884263No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC19330.12070484636514808No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC19000.11864418421820039No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18970.1184568512957506No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT18970.1184568512957506No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC18940.11826951837330081No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC18870.11783240822091796No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT18760.11714552083860208No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC18760.11714552083860208No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA18720.11689574360866901No Hit
CCCCTAACCTCCTGATCTTTGCTGCATCCACTTTACAAAGTGGGGTCCCA18640.1163961891488029No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT18620.11627130053383637No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC18400.11489752576920459No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA18130.11321152946715649No Hit
CCATCCTTCCTGTCTGCTTCTGTGGGAGACAGAGTCACCATCACTTGCCG18110.11308664085218996No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG17740.11077620147530921No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT17720.11065131286034269No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17620.11002686978551005No Hit
CTCCTGATCTTTGCTGCATCCACTTTACAAAGTGGGGTCCCATCAAGGTT17180.10727932025624647No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTC17090.10671732148889708No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC16990.10609287841406446No Hit
GTCTGCTTCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGG16930.10571821256916487No Hit
GTTAATGCCCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAG16860.10528110241678204No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC16830.10509376949433223No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT16660.10403221626711677No Hit
GGGTAACTATTAAGCTGTTGACAGTAATAAGTCGCAAAATCTTCAGGCTG16660.10403221626711677No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGAGGGTAACTATTAAGCTGTTGACAGTAATAAGTCGCAAAATCTTCAGG16640.10390732765215024No Hit
ATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATT16510.10309555165486782No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGAGGGTAACTATTAAGCTGTTGACAGTAATAAGTCGCAAAATCTTCAGG16380.1022837756575854No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTGTAAAGT16300.1017842211977193No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC16090.10047289074057075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3750.048.074051
GTATCAA31150.034.3968431
TCAATAC450.00491117730.29015727
ATCAACG35550.029.6380083
TCAACGC35850.029.4820374
TGTTAGA24500.028.98512370
TATCAAC36650.028.7583432
CAACGCA36900.028.6422215
AATGGTA4500.028.55571670
AACGCAG37650.028.071666
ATACGCT500.00832741927.1862358
CGAAACC1157.1524464E-626.71283369
CGCAGAG40000.026.4216238
ACGCAGA40050.026.3894637
TTTAGAC659.448172E-426.1446933
AGAGTAC40850.025.95665711
CAGAGTA41150.025.76661910
GCAGAGT41200.025.6528639
GTAGTGG13350.025.33031870
GTATAAC700.001448854324.2961851
Produced by FastQC (version 0.11.5)