FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633159
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23070.36436345372963186No Hit
AATCTATAGTGCCTCCAGTTTGGAAACTGGGGTCCCATCAAGATTCAGCGGCAGTGAGTCTGGGACAGATTTCA21970.3469902504742095No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21520.33988303096062755No Hit
GTTTCATACTTTCCCCCTTTTCGGCGGAGGGACCAGGGTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTT19630.31003270900358365No Hit
GTATGAAACTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTC18700.29534445534218107No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT17640.27860300493241036No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTT16730.2642306276938336No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15980.2523852618378638No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14690.23201123256559567No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG14570.2301159740286405No Hit
CTATAGATTAGGACCTTAGGAGCTTTCCCTGGTTTCTGCTGATACCAGGC13750.21716504069278017No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13540.21384833825310862No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTT13370.2111633886590888No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13160.20784668621941726No Hit
GTCTGGGACAGATTTCACTCTCACCATCACCAGCCTGCAGCCTGAAGATT12280.1939481236150793No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12160.19205286507812414No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12080.19078935938682068No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11980.1892099772726914No Hit
GCTTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCTCCTAAGGTCCTAATCTATAGTGCCTCCAGTTTGGAAA11630.1836821398732388No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11360.17941780816508965No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGCCATTGGTAGTGCTTTAGCCTGGTATCAGCAGAAA11240.1775225496281345No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG11170.17641698214824397No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGG11100.17531141466835345No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11090.17515347645694052No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11060.17467966182270173No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCT11020.17404790897705No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11020.17404790897705No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10980.17341615613139827No Hit
GTCCTAATCTATAGTGCCTCCAGTTTGGAAACTGGGGTCCCATCAAGATT10800.17057326832596553No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA10700.16899388621183623No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACCCTGGTCC10490.16567718377216467No Hit
CACCATCACCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGTTTCATACTTTCCCCCTTTTCGG10460.16520336913792585No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT10000.15793821141293105No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9830.1552532618189112No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT9120.1440396488085931No Hit
GATTTCACTCTCACCATCACCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGTTTCATACTTTC8820.13930150246620518No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC8320.13140459189555861No Hit
ACTTATTACTGTCAACAGTTTCATACTTTCCCCCTTTTCGGCGGAGGGAC8130.12840376587871294No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8120.1282458276673No Hit
GATTAGGACCTTAGGAGCTTTCCCTGGTTTCTGCTGATACCAGGCTAAAG8090.12777201303306118No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGG8060.12729819839882242No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8040.12698232197599654No Hit
GCTCCTGGGGCTTCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTCC7880.12445531059338964No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7750.12240211384502156No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7730.12208623742219568No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7640.12066479351947931No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC7560.11940128782817587No Hit
CTCCTGTGCAGCCTCTGGGTTCAGCGTCAGTAACAGCTACATGATTTGGGTCCGCCAGGCTCCAGGGAAGGGG7520.11876953498252414No Hit
GACCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG7480.11813778213687241No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT7460.11782190571404655No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7410.1170322146569819No Hit
GGCCATTGGTAGTGCTTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCTCCTAAGGTCCTAATCTATAGTGCCTC7120.1124520065260069No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7070.11166231546894224No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACTCACTGCCG7020.11087262441187759No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTTGGCTGAGCTGGGTTTTC6880.10866148945209655No Hit
GAAATACACGGCCGTGTCCTCGGCTCTGAGACTGTCCATTTGAAGATACA6670.10534478701242499No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT6590.10408128132112154No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT6570.10376540489829568No Hit
GACCAGGGTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT6570.10376540489829568No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6560.10360746668688277No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6550.10344952847546982No Hit
GAGTGAAATCTGTCCCAGACTCACTGCCGCTGAATCTTGATGGGACCCCA6510.10281777562981811No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC6500.10265983741840516No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC6500.10265983741840516No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6480.1023439609955793No Hit
GTCCCAGACTCACTGCCGCTGAATCTTGATGGGACCCCAGTTTCCAAACT6380.10076457888145No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)