FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633159
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35420.5594171448246017No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22670.3580459252731147No Hit
AATCTATAGTGCCTCCAGTTTGGAAACTGGGGTCCCATCAAGATTCAGCGGCAGTGAGTCTGGGACAGATTTCA20460.3231415805508569No Hit
GTTTCATACTTTCCCCCTTTTCGGCGGAGGGACCAGGGTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTT18760.29629208461065865No Hit
GTATGAAACTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTC18450.29139600005685773No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17050.2692846504590474No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16400.25901866671720686No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG15640.24701536264982416No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTT15010.23706525533080947No Hit
CTATAGATTAGGACCTTAGGAGCTTTCCCTGGTTTCTGCTGATACCAGGC13380.2113213268705017No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13060.2062673041052879No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12910.20389823093409395No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12650.19979183743735776No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCT12130.19157905044388535No Hit
GTCTGGGACAGATTTCACTCTCACCATCACCAGCCTGCAGCCTGAAGATT12130.19157905044388535No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTT12070.19063142117540774No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11970.18905203906127843No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11760.1857353366216069No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA11730.18526152198736812No Hit
GTCCTAATCTATAGTGCCTCCAGTTTGGAAACTGGGGTCCCATCAAGATT11600.183208325239No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11190.17673285857106982No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGCCATTGGTAGTGCTTTAGCCTGGTATCAGCAGAAA10680.16867800978901035No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10420.16457161629227415No Hit
GCTTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCTCCTAAGGTCCTAATCTATAGTGCCTCCAGTTTGGAAA10400.16425573986944828No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10030.15841202604716983No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC9940.15699058214445344No Hit
CACCATCACCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGTTTCATACTTTCCCCCTTTTCGG9880.15604295287597586No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9680.15288418864771725No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9620.15193655937923964No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACCCTGGTCC9620.15193655937923964No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGG9600.1516206829564138No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG9490.14988336263087157No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9420.14877779515098105No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9260.14625078376837414No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8990.141986452060225No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8820.13930150246620518No Hit
GTCCCAGACTCACTGCCGCTGAATCTTGATGGGACCCCAGTTTCCAAACT8720.13772212035207587No Hit
ACTTATTACTGTCAACAGTTTCATACTTTCCCCCTTTTCGGCGGAGGGAC8620.13614273823794654No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8500.13424747970099138No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8330.13156253010697155No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT8320.13140459189555861No Hit
GATTTCACTCTCACCATCACCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGTTTCATACTTTC8010.12650850734175775No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT7760.12256005205643447No Hit
GATTAGGACCTTAGGAGCTTTCCCTGGTTTCTGCTGATACCAGGCTAAAG7590.11987510246241466No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGG7550.11924334961676292No Hit
GAGTGAAATCTGTCCCAGACTCACTGCCGCTGAATCTTGATGGGACCCCA7450.11766396750263362No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT7440.1175060292912207No Hit
GACCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG7210.11387345042872328No Hit
CTCCTGTGCAGCCTCTGGGTTCAGCGTCAGTAACAGCTACATGATTTGGGTCCGCCAGGCTCCAGGGAAGGGG7160.11308375937165863No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACTCACTGCCG7000.11055674798905173No Hit
GAAATACACGGCCGTGTCCTCGGCTCTGAGACTGTCCATTTGAAGATACA6900.1089773658749224No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA6830.10787179839503189No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6720.10613447806948967No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC6700.10581860164666379No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT6630.10471303416677329No Hit
GCTCCTGGGGCTTCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTCC6620.10455509595536033No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6540.1032915902640569No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC6510.10281777562981811No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6490.10250189920699225No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6430.10155426993851466No Hit
GGCCATTGGTAGTGCTTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCTCCTAAGGTCCTAATCTATAGTGCCTC6390.10092251709286292No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6390.10092251709286292No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC6350.10029076424721121No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)