FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1552496
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTATTGGACGTTTGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT95970.618165843905556No Hit
CTATTATACTGTTGGCAGGAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTTATGGTGAGAGTGAATTCTGTC81590.5255408065463615No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54970.3540749863445703No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC53750.3462166730220239No Hit
GTATTAGTAGTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCGTCTA42050.2708541600107182No Hit
GAGTATTAGTAGTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCGT41240.26563675526378167No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39500.25442899691851056No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35320.2275046119281467No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33700.21706980243427357No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTGCAGGGAGAACTGGTTCTTGGACATGTCTACGGA33670.2168765652214241No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31810.20489585802475496No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31440.2025125990662778No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31120.20045140212921644No Hit
GTCTGCATCTGTGGGAGACACAGTCACCATCACTTGCCGGGCCAGTCAGA30940.1992919788521194No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG30300.19516958497799672No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC29990.19317280044521853No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29730.1914980779338562No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29150.18776215848543248No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29010.18686038482546816No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28870.18595861116550383No Hit
GAATTCACTCTCACCATAAGCAGCCTGCAGCCTGATGATTTTGCAACTTA28670.18467036307984047No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27700.17842235986437324No Hit
CACCATAAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTCCTGCCAACAGTATAATAGTTATTGGACGTTTGG26760.1723675938617555No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26680.1718522946274902No Hit
CCTTCCACCCTGTCTGCATCTGTGGGAGACACAGTCACCATCACTTGCCG26420.17017757211612783No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG25270.16277014562356362No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA24470.15761715328091022No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG24010.15465418268388453No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23700.15265739815110635No Hit
GCTTATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTAAACCTTGATGGGACCCCACTTTCTAAAGTAGA22490.1448634972328431No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22480.14479908482855994No Hit
GTCTCTGGTGACTCCATCAATAATTACCACTGGACCTGGATCCGGCAGCC21760.1401613917201719No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21280.13706959631457988No Hit
CAGTATAATAGTTATTGGACGTTTGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT21200.13655429708031452No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20880.13449310014325316No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20520.13217425358905918No Hit
CTACTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTGTGTCTCCCACAGATGCAGACAGGGTGGAAGGAGA20290.13069276829054632No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC20270.13056394348198No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT20210.13017746905628097No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC19890.1281162721192196No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19750.12721449845925528No Hit
ACTTTAGAAAGTGGGGTCCCATCAAGGTTTAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATAAGCAGC19590.1261838999907246No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19590.1261838999907246No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC19290.12425152786222961No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19010.12244798054230092No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT18990.12231915573373457No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCAAACGTC18580.11967824715812474No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18560.11954942234955838No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18000.11594232770970102No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT17920.11542702847543568No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17370.11188434623986149No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTAAACCTTGATGGGACCCCA17340.11169110902701199No Hit
GTGTATTACTGTGCGAGATGGCGTGGATATGGTTATGGTCTTGACTATTGGGGCCCGGGAACCCTGGTTACCGT17180.1106605105584813No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17100.11014521132421598No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC17090.1100807989199328No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC16780.10808401438715462No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16660.10731106553575662No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC16650.10724665313147344No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTGGGAGACACAGTCACCATCACTT16420.1057651678329606No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16250.10467015696014675No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16130.10389720810874874No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16080.10357514608733293No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16060.10344632127876657No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC15920.10254454761880225No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15860.10215807319310323No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15820.10190042357597057No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG15640.10074100029887356No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15570.1002901134688914No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT15550.10016128866032505No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)