FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1251929
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCGTGGGATGACAGCCTGAGTGGTCCGGTGTTCGG85710.6846234890317262No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC61640.4923601897551698No Hit
GTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCTGCGACCC51930.41479988082391256No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG49300.3937922997230674No Hit
AGTCTGTACTGACTCAGCCACCCTCAGCGTCTGCGACCCCCGGGCAGAGG46560.37190607454576097No Hit
GTACTGACTCAGCCACCCTCAGCGTCTGCGACCCCCGGGCAGAGGGTCAC46470.3711871839377473No Hit
GTAATAATCAGCCTCATCTTCGGACCGGAGCCCACTGATGGCCAGGGAGG45820.3659951962132038No Hit
GCCTTGGGCTGACCTAGGACGGTCAGGTTGGTCCCTCCGCCGAACACCGGACCACTCAGGCTGTCATCCCACG44940.35896604360151413No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCT39080.312158277346399No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT38970.3112796332699378No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG37310.298020095388796No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35260.2816453648729281No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA35210.2812459812018094No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGGTTGGTCCCTC34130.2726192939056448No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32480.2594396327587267No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT32470.259359756024503No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA32420.25896037235338426No Hit
CCCTCAGCGTCTGCGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG31200.24921541077808726No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG31170.248975780575416No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30890.24673923201715114No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCAC30720.24538132753534744No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT30260.24170699776105514No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG29800.2380326679867628No Hit
GACCTAGGACGGTCAGGTTGGTCCCTCCGCCGAACACCGGACCACTCAGG29170.23300043373066684No Hit
GTATCAACGCAGAGTACGGGAGCTTCACCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG28490.22756881580345215No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27430.21910188197573505No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC27150.2168653334174702No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26520.21183309916137416No Hit
CCCCTGAGGGCCGCTGATTATCCCTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGATACCAGTATA26270.2098361808057805No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25890.20680086490527816No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG25690.20520333022080325No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG25680.2051234534865795No Hit
GATTATCCCTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGA25630.2047240698154608No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24870.19865343801445612No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC23690.1892279833760541No Hit
GCTCTAACATCGCAAATAATTTTGTATACTGGTATCAGCAGCTCCCAGGA23650.1889084764391591No Hit
GGGATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG23600.18850909276804037No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT23290.18603291400710426No Hit
GCTGATACCAGTATACAAAATTATTTGCGATGTTAGAGCTGCTTCCAGAA23240.1856335303359855No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGGTTGGTCCCTCCGCCGAACAC23070.18427562585418183No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22820.18227870749858818No Hit
GAGTACGGGAGCTTCACCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT22180.17716659650826844No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC22160.17700684303982095No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22090.17644770590025471No Hit
CTCTTGTTCTGGAAGCAGCTCTAACATCGCAAATAATTTTGTATACTGGTATCAGCAGCTCCCAGGAACGGCCCC22020.1758885687606885No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC21740.17365202020242362No Hit
GTACGGGAGCTTCACCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC21330.17037707409925001No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT20990.16766126513564267No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20420.16310829128488916No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20300.1621497704742042No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA19680.15719741295233197No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19430.1552004945967383No Hit
TTACTGTGCAGCGTGGGATGACAGCCTGAGTGGTCCGGTGTTCGGCGGAG18790.15008838360641857No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC18680.14920973952995736No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18640.1488902325930624No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGGCTCCTCAGCTCCATGTAGGCTGTGGTCGTGGATGTGTCTGTGGT18200.14537565628721755No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18200.14537565628721755No Hit
CATCTATAGGGATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC17940.14329886119740018No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAAGATG17630.14082268243646404No Hit
GGCCATCAGTGGGCTCCGGTCCGAAGATGAGGCTGATTATTACTGTGCAG17430.13922514775198913No Hit
ATGTTAGAGCTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGT16940.1353111877750256No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG16900.1349916808381306No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC16750.1337935298247744No Hit
GGACCAACCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG16460.13147710453228578No Hit
GGTTGGTCCCTCCGCCGAACACCGGACCACTCAGGCTGTCATCCCACGCTGCACAGTAATAATCAGCCTCATCTT16320.13035883025315334No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTA16020.12796252822644094No Hit
GTACAGACTGGGCCCAGGACCCTGCACAGTGAGTGAGGAGGGTGAGGAGG15900.12700400741575602No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC15850.12660462374463727No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15700.12540647273128108No Hit
GTTTGGGGGCCGTTCCTGGGAGCTGCTGATACCAGTATACAAAATTATTT15470.12356930784413493No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15380.12285041723612122No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG15300.12221140336233126No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC15220.1215723894885413No Hit
GGTATCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTATAGGGATAATCAGCGGCCCTCAGGGGTCC15180.12125288255164629No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGGTTGGTC15170.12117300581742255No Hit
GGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT15140.12093337561475133No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTATAGGGATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG15070.12037423847518508No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC15020.11997485480406636No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT14990.11973522460139513No Hit
GCGTGGGATGACAGCCTGAGTGGTCCGGTGTTCGGCGGAGGGACCAACCTGACCGTCCTAGGTCAGCCCAAGG14820.11837732011959144No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGCTCTAACATCGCAAATAATTTT14780.11805781318269645No Hit
ACCTAGGACGGTCAGGTTGGTCCCTCCGCCGAACACCGGACCACTCAGGC14660.11709929237201153No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA14210.11350483933194294No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14190.11334508586349547No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC14180.11326520912927171No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCTGCGACCCCCGGGCAGAGGGTCAC14040.11214693485013927No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13790.11015001649454562No Hit
GTCCGGTGTTCGGCGGAGGGACCAACCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC13740.10975063282342688No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAG13640.10895186548118942No Hit
GGTATCAACGCAGAGTACGGGAGCTTCACCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG13600.10863235854429446No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13490.10775371446783324No Hit
GATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGATACCAGTATACAA13480.10767383773360949No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGT13420.10719457732826702No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC13340.10655556345447706No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC13320.10639580998602957No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC13300.10623605651758207No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTA13270.10599642631491082No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG13230.10567691937801585No Hit
GATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12980.1036800010224222No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG12810.10232209654061851No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG12790.10216234307217102No Hit
GTGTATTACTGTGCGAGACTTTCAAGCAGTGGCTGGCCCCTTGATGCTTT12710.10152332919838106No Hit
CTCCTCATCTATAGGGATAATCAGCGGCCCTCAGGGGTCCCTGACCGATT12670.10120382226148608No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC12650.1010440687930386No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG12620.10080443859036735No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)