FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences850252
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25980.3055564703170354No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25920.3048507971754256No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24200.28462150044927853No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20350.23934080719598427No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19080.22440405903191052No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17740.20864402553595873No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG17600.20699745487220259No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16770.19723564307993396No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15400.18112277301317725No Hit
GTAGGAGACAGCGTCACCATCAGTTGCCAGGCGAGTCAGGACATTAGGAACTATTTAAATTGGTATCAGCAGAAA14970.1760654488316405No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14870.17488932692895753No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14050.16524512732695718No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA13830.16265765914105465No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13700.1611287006675668No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13460.1583060081011277No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13250.15583615210549343No Hit
GTATTACTGTGCCTACGAGACCACGATTCGGCGGTGCTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTC13180.15501286677361534No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12890.15160211325583473No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12720.1496027060212737No Hit
CAGTAATACATGGCCGTGTCCTCAGATCTTAGGCTGCTCAGTTCCATGTA12650.14877942068939562No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12590.14807374754778582No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12580.14795613535751753No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12500.14701523783537115No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGCGTCACCATCAGTTGCCAGGCGAGTCAGGACATTAGGAA12090.14219313803437097No Hit
GTATGATAATATGATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT11970.14078179175115144No Hit
GATTTTACCTTAACCATCAGCAGCCTGCAACCTGAAGATATTGCAACATA11740.1380767113749806No Hit
ATATTACTGTCAACAGTATGATAATATGATGTACACTTTTGGCCAGGGGA11640.13690058947229763No Hit
TCCCTATCCTTGGTACACCAGTCTACGCACAGAAGTTCCGGGGCAGAGTCTCCATTACCGCGGACAGATCGACGA11520.13548924318907807No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11470.1349011822377366No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11370.1337250603350536No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11240.13219610186156575No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11220.13196087748102917No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11170.13137281652968769No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10900.12819728739244365No Hit
CAGTAATATGTTGCAATATCTTCAGGTTGCAGGCTGCTGATGGTTAAGGTAAAATCTGTCCCAGATCCACTTCCA10330.12149339254715072No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTACTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10140.11925876093205308No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9890.11631845617534566No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9850.11584800741427247No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9840.11573039522400418No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9770.11490710989212609No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT9710.11420143675051632No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9680.11384860017971143No Hit
ACATGGAACTGAGCAGCCTAAGATCTGAGGACACGGCCATGTATTACTGT9660.11361337579917483No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9560.11243725389649187No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9560.11243725389649187No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9370.11020262228139423No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA9320.10961456133005273No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC9060.106556644383077No Hit
TTATCATACTGTTGACAGTAATATGTTGCAATATCTTCAGGTTGCAGGCTGCTGATGGTTAAGGTAAAATCTGTC9040.10632142000254043No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT9000.10585097124146724No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG8990.10573335905119895No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8860.10420440057771108No Hit
GTAATATGTTGCAATATCTTCAGGTTGCAGGCTGCTGATGGTTAAGGTAAAATCTGTCCCAGATCCACTTCCA8820.10373395181663789No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG8810.1036163396263696No Hit
GGCCAAAAGTGTACATCATATTATCATACTGTTGACAGTAATATGTTGCA8760.1030282786750281No Hit
ACATATTACTGTCAACAGTATGATAATATGATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA8690.10220499334315004No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8640.10161693239180854No Hit
GTTCCATGTAGGCTGTGGTCGTCGATCTGTCCGCGGTAATGGAGACTCTG8540.10044081048912559No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)